Abstract:A recombinant Pichia strain GS115-Ch-Glu expressing both β-glucosidase and cyanide hydratase was constructed to remove cyanogenic glycosides in edible plants. The β-glucosidase could hydrolyze cyanogenic glycosides into cyanide and its gene (Glu) was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). A cyanide hydratase could catalyze cyanide to formamide and its gene (Ch) was obtained by C (PCR). Both Glu and Ch genes were integrated into the genome of Pichia strain GS115 by homologous rec… Show more
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