Purpose: White light optical profiling (WLOP), no usually applied to contact lenses (CL), is a well-established technique for non-contact measurement of surface topography over large areas at moderate lateral and high vertical resolution (sub-nanometer). The aim of this study is to analyze surface properties of five hydrogel CL evaluating representative roughness parameters as Roughness Average (R a ) and Root-Mean-Square Roughness (R q ), that depend on the sampling length, obtained by WLOP. Methods: Lenses used in this study were two highly biocompatible CL and three silicone-hydrogel CL. Unworn CL surface roughness and topography were studied with WLOP (Wyco NT1100) in VSI mode (Vertical Scanning Interferometry). 50X and 20X objectives were used. Height roughness parameters Ra and Rq were calculated for 625, 2500, 10829 and 67646 µm 2 areas using the WycoVision®32 analytical software package. Results: High quality 3D topographic images were recorded at randomly different surface locations. Surface topography and Ra and Rq show different values depending on the contact lens and the measurement areas, with the highest roughness scores in biocompatible CL (R a /R q for 625, 2500, 10829 and 67646 µm 2 areas were: Hioxifilcon