Production, characterization and biological activities of acidic exopolysaccharide from marine Bacillus amyloliquefaciens 3MS 2017

El-Newary, Samah A.; Ibrahim, Abeer Y.; Asker, Mohsen S.; Mahmoud, Manal G.; Awady, Mohamed E. El

doi:10.1016/j.apjtm.2017.07.005

Cited by 42 publications

(29 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The IC 50 value of Graesiella EPSs was estimated to be 0.22 g L −1 , a value lower than the IC 50 of L-ascorbic acid which was 0.53 g L −1 . Graesiella EPSs showed a significant (p < 0.05) superoxide radical scavenging ability which has already been reported for bacterial EPSs (El-Newary et al 2017). Previous reports on the scavenging capacity of chlorophyte EPSs have shown the possibility of sulfate content being correlated with the potent activity (Ghasemi et al 2007;Raposo et al 2014).…”

Section: Radical Scavenging Abilitysupporting

confidence: 75%

Production of exopolymer substances from the thermophilic chlorophyte Graesiella: industrial and ecological applications

et al. 2020

View full text Add to dashboard Cite

Microalgal extracellular polymeric substances (EPSs) are heteropolysaccharides that have characteristics suitable for industrial and biotechnological applications. Notably, they have strong anionic nature and high hydrophobicity. Nevertheless, systematic studies to demonstrate the viability of the production of EPSs on an industrial scale are still crucial. In this research, the chlorophyte Graesiella was grown on a raceway pond to view its EPS valorization. The biomass production achieved a maximum of 1.98 g L −1 and an EPS production of 1.6 g L −1 after six production days. The Graesiella EPSs with a molecular weight above 100 kDa are sulfated exopolymers containing mainly polysaccharide (70%) and protein (16%). The EPSs produced more stable emulsions with hydrocarbons and oils than Tween-20. The emulsification indices with n-hexane (88%) and maize oil (28%) indicate the EPSs' strong emulsion-stabilizing capacity. The EPSs showed a peak flocculating percentage of 95% to kaolin suspension, with better flocculation performance than Al 2 (SO 4 ) 3 and alginate. Moreover, Graesiella EPSs had a significant effect on antimicrobial activity, significantly inhibiting fungal growth (71% for Botytis cinerea and 87% for Fusarium oxysporum), spore germination (100% of inhibition at a concentration of 1.8 g L −1 ), and mycelium growth (68% of inhibition). Also, Graesiella EPSs acted as a bactericide against Vibrio anguilaruim and Listonella anguilaruim (100% inhibition). EPSs were also found to have potent antioxidant activity compared with L-ascorbic acid. The obtained results open new perspectives to the further exploration of Graesiella sp. as a potential EPS producer, making it a promising candidate for numerous industrial applications.

show abstract

Section: Radical Scavenging Abilitysupporting

confidence: 75%

Production of exopolymer substances from the thermophilic chlorophyte Graesiella: industrial and ecological applications

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Molecular weight of EPS. The molecular weight of the EPS from strain GSBa-1 was determined to be 54 kDa by GPC-RID-20 system ( Fig.2A), which was higher than that (37.6 kDa) of the EPS from B.Amyloliquefaciens 3MS [22]. The molecular weight of the EPS varied with the producing strain, and it might be affected by fermentation time and medium composition [23].…”

Section: Characterization Of Epsmentioning

confidence: 92%

Characterization and Antioxidant Activity of the Exopolysaccharide Produced by Bacillus amyloliquefaciens GSBa-1

Zhao¹,

Zhang²,

Jiang³

et al. 2018

Journal of Microbiology and Biotechnology

View full text Add to dashboard Cite

The exopolysaccharide (EPS) produced by Bacillus amyloliquefaciens GSBa-1 was isolated and purified by ethanol precipitation, DEAE-cellulose and Sepharose CL-6B chromatography. The molecular weight of the purified EPS was determined to be 54 kDa. Monosaccharide analysis showed that the EPS was composed of predominantly glucose, and it was further confirmed by NMR spectroscopy to be α-glucan consisted of a trisaccharide repeating unit with possible presence of two α-(1→3) and one α-(1→6) glucosidic linkages. Microstructural analysis showed that the EPS appeared as ellipsoid or globose with smooth surface. The EPS had a degration temperature at 240℃. Furthermore, the EPS had strong DPPH and hydroxyl radical scavenging activities, and moderate scavenging activities on superoxidant anion and metal ion chelating activity. This is the first characterization of a glucan produced by B. amyloliquefaciens with strong antioxidant activity. The results of this study suggested the potential of the EPS from B. amyloliquefaciens GSBa-1 to serve as a natural antioxidant for application in functional products.

show abstract

“…Its hydroxyl radical (45.65% at 0.1 mg/mL) and superoxide radical (67.31% at 0.25 mg/mL) scavenging activities were comparable to vitamin C (Table 1). It is noticeable that some EPS not only have antioxidant activity, but also inhibit the growth of cancer cells (Tables 1 and 2) [62,63,132]. For instance, El-Newary et al [62] found an exopolysaccharide (BAEPS) produced by the marine Bacillus amyloliquefaciens 3MS 2017, which contains 22.8% sulfate and has a molecular weight of 37.6 kDa.…”

Section: Bacterial Polysaccharidesmentioning

confidence: 99%

“…It is noticeable that some EPS not only have antioxidant activity, but also inhibit the growth of cancer cells (Tables 1 and 2) [62,63,132]. For instance, El-Newary et al [62] found an exopolysaccharide (BAEPS) produced by the marine Bacillus amyloliquefaciens 3MS 2017, which contains 22.8% sulfate and has a molecular weight of 37.6 kDa. The IC 50 values of BAEPS on radical scavenging of DPPH and hydrogen peroxide were 0.21 and 30.04 µg/mL, respectively.…”

Section: Bacterial Polysaccharidesmentioning

confidence: 99%

The Antioxidant Activity of Polysaccharides Derived from Marine Organisms: An Overview

et al. 2019

View full text Add to dashboard Cite

Marine-derived antioxidant polysaccharides have aroused extensive attention because of their potential nutritional and therapeutic benefits. However, the comprehensive comparison of identified marine-derived antioxidant polysaccharides is still inaccessible, which would facilitate the discovery of more efficient antioxidants from marine organisms. Thus, this review summarizes the sources, chemical composition, structural characteristics, and antioxidant capacity of marine antioxidant polysaccharides, as well as their protective in vivo effects mediated by antioxidative stress reported in the last few years (2013–2019), and especially highlights the dominant role of marine algae as antioxidant polysaccharide source. In addition, the relationships between the chemical composition and structural characteristics of marine antioxidant polysaccharides with their antioxidant capacity were also discussed. The antioxidant activity was found to be determined by multiple factors, including molecular weight, monosaccharide composition, sulfate position and its degree.

show abstract

Production, characterization and biological activities of acidic exopolysaccharide from marine Bacillus amyloliquefaciens 3MS 2017

Abstract: BAEPS has anti-tumor activity with a good margin of safety. The anti-tumor activity of BAEPS may be due to its content from sulfated groups and uronic acids and they have antioxidant and anti-inflammatory properties.

Cited by 42 publications

References 39 publications

Production of exopolymer substances from the thermophilic chlorophyte Graesiella: industrial and ecological applications

Production of exopolymer substances from the thermophilic chlorophyte Graesiella: industrial and ecological applications

Characterization and Antioxidant Activity of the Exopolysaccharide Produced by Bacillus amyloliquefaciens GSBa-1

The Antioxidant Activity of Polysaccharides Derived from Marine Organisms: An Overview

Contact Info

Product

Resources

About