Characterization and Antioxidant Activity of the Exopolysaccharide Produced by Bacillus amyloliquefaciens GSBa-1

Abstract

The exopolysaccharide (EPS) produced by Bacillus amyloliquefaciens GSBa-1 was isolated and purified by ethanol precipitation, DEAE-cellulose and Sepharose CL-6B chromatography. The molecular weight of the purified EPS was determined to be 54 kDa. Monosaccharide analysis showed that the EPS was composed of predominantly glucose, and it was further confirmed by NMR spectroscopy to be α-glucan consisted of a trisaccharide repeating unit with possible presence of two α-(1→3) and one α-(1→6) glucosidic linkages. Microstructural analysis showed that the EPS appeared as ellipsoid or globose with smooth surface. The EPS had a degration temperature at 240℃. Furthermore, the EPS had strong DPPH and hydroxyl radical scavenging activities, and moderate scavenging activities on superoxidant anion and metal ion chelating activity. This is the first characterization of a glucan produced by B. amyloliquefaciens with strong antioxidant activity. The results of this study suggested the potential of the EPS from B. amyloliquefaciens GSBa-1 to serve as a natural antioxidant for application in functional products.

Biomedical and therapeutic potential of exopolysaccharides by Lactobacillus paracasei isolated from sauerkraut: Screening and characterization

“…The hydroxyl free radical scavenging results of the exopolysaccharide in the potent isolate of B. amyloliquefaciens was 89.75 ± 2.68%. The bacterial exopolysaccharides have free radical scavenging abilities on both DPPH radicals and hydroxyl radicals with various bioactivities viz , prevention of chain initiation, decomposition of peroxides ( Zhao et al, 2018 , Wang et al, 2017 ). The previous findings are in accordance with our reports which reveal the H 2 O 2 free radical scavenging results of EPS from the candidate isolate was 68.65 ± 0.22%.…”

Structural Characterisation and Assessment of the Novel Bacillus amyloliquefaciens RK3 Exopolysaccharide on the Improvement of Cognitive Function in Alzheimer’s Disease Mice

“…The 1 H NMR spectrum ( Figure 5 ) displayed the characteristic anomeric (H1) signals in the anomeric region 4.0–5.4 ppm. In this anomeric region, a doublet was found at 4.55 ppm and another doublet was found at 4.58 ppm and these two peaks were identified β- d -Galactopyranose (β- d -Gal p ), which should contain a linkage at its 6th/2nd position, and a Terminal (0) β- d -Gal p [ 69 ]. Since these peaks are slightly overlapped with the water signal, the intensities are incorrect and not shown in the figure (Magnified portion).…”

Exopolysaccharide from Lactobacillus plantarum HY7714 Protects against Skin Aging through Skin–Gut Axis Communication

“…Most lactobacillus EPS molecules are heteropolymers consisting of repeated copies of oligosaccharide units [15]. EPS, which are produced either intracellularly or extracellularly, contribute to biological activity via specific composition, size, and branching structure, which can differ even among members of the same LAB strain [33].…”

“…Exopolysaccharide (EPS) consists of long-chain polysaccharides with various branches and repeating units of sugars or sugar derivatives [15]. Recent studies showed that EPS from marine microorganisms has potential applications as a therapeutic food [12,16].…”