Production, characterization and antioxidant activities in vitro of exopolysaccharides from endophytic bacterium Paenibacillus polymyxa EJS-3

Liu, Jun; Luo, Jianguang; Ye, Hong; Sun, Yi; Lu, Zhaoxin; Zeng, Xiaoxiong

doi:10.1016/j.carbpol.2009.03.046

Cited by 183 publications

(137 citation statements)

References 25 publications

Supporting

Mentioning

123

Contrasting

Unclassified

Order By: Relevance

“…Shu and Lung (2004) reported the EPS production of Antodia spp., and it was found to be high in the culture medium containing pH value of 5.0. In P. polymyxa EJS-3, the maximum production of EPS was found at pH 8.0 (Liu, 2009). …”

Section: Resultsmentioning

confidence: 94%

Production of Antibacterial and Larvicidal Extracellular Polysaccharide from Pseudomonas aeruginosa Strain b01 Isolated from Wastewater

Benit¹,

Roslin²

2017

Int.J.Curr.Res.Aca.Rev.

View full text Add to dashboard Cite

Article InfoThe aim of this study is to optimize extracellular polysaccharide (EPS) production from Pseudomonas aeruginosa B01 with potent antibacterial and larvicidal activity. EPS producing P. aeruginosa B01 was isolated from wastewater. Process parameters were optimized to enhance the production of EPS. EPS was fractionated with two-, three-volume of various solvent and antibacterial and larvicidal activities were evaluated. The bacterial cell growth was maximum after 48 h fermentation period, however, EPS production was maximum after 72 h. EPS production was found to be high when this organism was grown in the medium containing 1% maltose, 1% ammonium sulphate and 0.1% Mn 2+ ions. EPS production was maximum at pH 7.5 and at 30 °C. Ethanol precipitated fractions showed high activity than other tested solvents. It was further subjected to Thin Layer Chromatography. EPS showed potent larvicidal activity against mosquito larva and showed potent activity against various pathogenic bacteria.

show abstract

Section: Resultsmentioning

confidence: 94%

Production of Antibacterial and Larvicidal Extracellular Polysaccharide from Pseudomonas aeruginosa Strain b01 Isolated from Wastewater

Benit¹,

Roslin²

2017

Int.J.Curr.Res.Aca.Rev.

View full text Add to dashboard Cite

show abstract

“…Many researchers have evaluated the effects of process parameters on the maximal production of EPSs to optimize the process conditions, such as temperature, pH and medium composition (Rafigh et al, 2014;Raza et al, 2011). Studies of EPS have demonstrated that the composition of the culture medium plays an important role in EPS production (Liu et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

“…In the present study EPS production was high when the organism was incubated at 40 °C (2.49 mg/ml). Fermentation temperature is one of the critical factors for the production of EPS (Liu et al, 2009). Rafigh et al, (2014) reported that EPS production increased rapidly when the incubation temperature varied from 30 to 40 °C and then slightly increased as the fermentation proceeded from 40 to 50 °C.…”

Section: Resultsmentioning

confidence: 99%

“…Reports suggest that nitrogen limitation and maximum amounts of carbon in the medium could yield a maximum amount of EPS in the culture medium (Degeest and de Vuyst, 1999). The carbon sources such as, glucose, sucrose were mainly used for the production of EPS (Liu et al, 2009). Many authors have reported that the production of EPS is the culture medium containing carbon and nitrogen sources.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Untitled

2017

Int.J.Curr.Res.Aca.Rev.

View full text Add to dashboard Cite

Article InfoExtracellular polymeric substances (EPSs) are produced by a wide array of bacteria for protection against aggregation, expression of virulence and adhesion. Growth associated EPS production was determined by Paenibacillus sp. in submerged fermentation. To improve the productivity and efficiency of EPS from Paenibacillus sp. the physical factors and nutrient components were optimized. The bacterial growth (2.093 OD at 600 nm) was maximum after 36 h of incubation and EPS production (1.9 mg/ml) was high after 3 days of incubation at 37 °C. EPS production was found to be maximum at pH 7.0 (2.31 mg/ml), and at 40 °C (2.49 mg/ml). Supplementation of 1% sucrose (4.30 mg/ml) supported more EPS production. Among the nitrogen sources, addition of yeast extract (0.1%) supported maximum EPS production (5.36 mg/ml). Among inorganic sources, NH 4 Cl significantly influenced on EPS production.

show abstract

“…The crude Beta-glucan was prepared according to the reported method with some modifications [10]. Briefly, the mycelia of lower fungi were harvested from the liquid medium by filtering through a filter paper, washed three times with distilled water, and dried at 60°C to a constant weight.…”

Section: 1preparation Of β-Glucanmentioning

confidence: 99%

Optimization of ?-Glucan Production from Lower Fungi using Central Composite Design and its Biological Application

Paulraj¹,

Saravanan²

2012

IJCA

View full text Add to dashboard Cite

Production of β-glucan is mainly used for cancer therapy. The most well-known are those occur in fungal grains such as cereal and other micro-organisms. The most important beta glucan from a nutritional and ingredient standpoint is that derived from fungal grain. Optimization of medium ingredients for the production of mycelial β-glucan from lower fungi in submerged fermentation and antioxidant activity of β-glucan was carried out.

show abstract

Production, characterization and antioxidant activities in vitro of exopolysaccharides from endophytic bacterium Paenibacillus polymyxa EJS-3

Cited by 183 publications

References 25 publications

Production of Antibacterial and Larvicidal Extracellular Polysaccharide from Pseudomonas aeruginosa Strain b01 Isolated from Wastewater

Production of Antibacterial and Larvicidal Extracellular Polysaccharide from Pseudomonas aeruginosa Strain b01 Isolated from Wastewater

Untitled

Optimization of ?-Glucan Production from Lower Fungi using Central Composite Design and its Biological Application

Contact Info

Product

Resources

About