Optimization of ?-Glucan Production from Lower Fungi using Central Composite Design and its Biological Application

Paulraj, Balaji; Saravanan, T.

doi:10.5120/7647-0737

Cited by 3 publications

(3 citation statements)

References 12 publications

(4 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The commonly used yeast S. cerevisiae can produce a β-glucan yield of about 6–12% ( Zechner-Krpan et al, 2010 ). A. niger can produce a yield of 9–11% ( Paulraj et al, 2012 ). In a recent approach, a maximum fungal biomass of 0.76 mg/g with β-glucan at 9.34% (w/w) was obtained during solid-state fermentation of brown rice by A. oryzae KCCM 12698 ( Ji and Ra, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto

Utama

Dio

Sulistiyo

et al. 2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

β-glucan is a natural polysaccharide derivative composed of a group of glucose monomers with β-glycoside bonds that can be synthesized intra- or extra-cellular by various microorganisms such as yeasts, bacteria, and moulds. The study aimed to discover the potential of various microorganisms such as Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto in producing β-glucan. The experimental method used and the data were analyzed descriptively. The four microorganisms above were cultured under a submerged state in Yeast glucose (YG) broth for 120 h at 30 °C with 200 rpm agitation. During the growth, several parameters were examined including total population by optical density, the pH, and glucose contents of growth media. β-glucan was extracted using acid-alkaline methods from the growth media then the weight was measured. The results showed that S. cerevisiae, A. oryzae X. campestris, and B. natto were prospective for β-glucans production in submerged fermentation up to 120 h. The highest β-glucans yield was shown by B. natto (20.38%) with the β-glucans mass of 1.345 ± 0.08 mg and globular diameter of 600 μm. The highest β-glucan mass was achieved by A. oryzae of 82.5 ± 0.03 mg with the total population in optical density of 0.1246, a final glucose level of 769 ppm, the pH of 6.67, and yield of 13.97% with a globular diameter of 1400 μm.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Moulds belonging to the genus Aspergillus can be used for β-glucan production ( Odabasi et al 2006 ). Paulraj et al (2012) obtained a dry weight of 0.78 g of A. niger mycelia and a dry weight of 0.5 g of β-glucan. Another species of Aspergillus that can be used for β-glucan production is A. oryzae .…”

Section: Introductionmentioning

confidence: 99%

Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto

Utama

Dio

Sulistiyo

et al. 2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…Thus, it has demonstrated that an increase in β-glucan concentration increases the antioxidant activity at a maximum of 78.31% at 100 µg/ml of concentration. This value is higher than that of ascorbic acid which resulted in a maximum of 51.80% radical scavenging activity at the same concentration (Paulraj and Saravanan, 2012).…”

Section: Antioxidant Propertiesmentioning

confidence: 94%

Structural Characterization, Technological Functionality, and Physiological Aspects of Fungal β-D-glucans: A Review

Borchani

Fonteyn

Jamin

et al. 2015

Critical Reviews in Food Science and Nutrition

View full text Add to dashboard Cite

β-D-glucans are a (1→3)-linked glucose polymer with (1→6)-linked side chains and a major component of fungal cell walls. They exhibit structural integrity to the fungal cell wall. In addition, β-glucans are widely used as food adjuvant in food and pharmaceutical industries because of their physico-chemical properties. Several studies have focused on different isolation processes of (1→3) (1→6)-β-glucan that could affect the physico-chemical and functional properties of β-glucan such as chemical composition, solubility, viscosity, hydration properties, and oil binding capacity. Immunological activity is one of the most important properties of β-glucans. Thus, they are effective in inhibiting growth of cancer cells and metastasis and preventing bacterial infection. In humans, β-glucans reduce blood cholesterol, improve glucose absorption by body cells, and so help wound healing. This review described the prebiotic potentiality of fungal β-D-glucans with the objective to detail the methodologies applied for their extraction, their structure and techno-functional properties, and finally their biological effects.

show abstract

Nutraceutical Enrichment of Animal Feed by Filamentous Fungi Fermentation

et al. 2022

View full text Add to dashboard Cite

There is an urgent need for improvements in animal production, particularly for ruminants, such that more sustainable and efficient processes are developed for obtaining more nutritious and efficient feeds. Filamentous fungi can add value to residual plant biomass, and may also have the potential to produce metabolites and enrich plant biomasses used in animal nutrition, converting them into nutraceutical sources. Thus, in this work, filamentous fungal fermentation of ruminant feed biomasses commonly used in Brazil was performed, and the enrichment for bioactive metabolites was tested. For this, Fistulina hepatica, Ganoderma lucidum, Pleurotus pulmonarius, Panus lecomtei, and Aspergillus terreus were grown for 28 days on different substrates: starchy grains- (sorghum, oat, and corn), fibrous substrates (coast-cross, rice husk, and moringa plant) and protein-rich substrates (cottonseed cake and pigeon pea plant). Fermented substrates were evaluated for laccase activity, crude protein, β-glucan, and lovastatin content. The highest growth rate was observed for G. lucidum in oat substrate (OT-01) (0.708 ± 0.035 cm/day) and F. hepatica in oat + coast-cross + pigeon pea treatment (OT-10) (0.607 ± 0.012 cm/day). High laccase activity was observed for P. lecomtei grown in starchy grain + moringa + pigeon pea substrate, reaching an activity of 416.8 ± 20.28 U/g. A. terreus growth in ST-09 (sorghum + pigeon pea) showed higher protein (15.3 ± 0.46%), β-glucan (503.56 ± 8.6 mg/g) and lovastatin (1.10 ± 0.17 mg/g) content compared to untreated substrates. These results demonstrate that filamentous fungi are an alternative for nutraceutical enrichment of ruminant feed biomasses. To the best of our knowledge, this is the first report in which P. lecomtei and F. hepatica are evaluated for their ability to be cultivated in ruminant feed substrates from Brazil.

show abstract

Optimization of ?-Glucan Production from Lower Fungi using Central Composite Design and its Biological Application

Cited by 3 publications

References 12 publications

Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto

Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto

Structural Characterization, Technological Functionality, and Physiological Aspects of Fungal β-D-glucans: A Review

Nutraceutical Enrichment of Animal Feed by Filamentous Fungi Fermentation

Contact Info

Product

Resources

About