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Abstract

Article InfoExtracellular polymeric substances (EPSs) are produced by a wide array of bacteria for protection against aggregation, expression of virulence and adhesion. Growth associated EPS production was determined by Paenibacillus sp. in submerged fermentation. To improve the productivity and efficiency of EPS from Paenibacillus sp. the physical factors and nutrient components were optimized. The bacterial growth (2.093 OD at 600 nm) was maximum after 36 h of incubation and EPS production (1.9 mg/ml) was high after 3 days of incubation at 37 °C. EPS production was found to be maximum at pH 7.0 (2.31 mg/ml), and at 40 °C (2.49 mg/ml). Supplementation of 1% sucrose (4.30 mg/ml) supported more EPS production. Among the nitrogen sources, addition of yeast extract (0.1%) supported maximum EPS production (5.36 mg/ml). Among inorganic sources, NH 4 Cl significantly influenced on EPS production.