Production, Characterization, and Antimicrobial Activity of Mycocin Produced by<i>Debaryomyces hansenii</i>DSMZ70238

Al-Qaysi, Safaa A. S.; Al-Haideri, Halah H.; Thabit, Zaid A.; Al-Kubaisy, Wijdan Hameed Abd Al-Razzaq; Ibrahim, Jamal Abd Al-Rahman

doi:10.1155/2017/2605382

“…More than 11 different killer toxins have been studied and identified, many types of yeasts defined as killer toxins such genera as Saccharomyces, Williopsis, Debryomyces, Pichia, Torulaspora, Ustilago, Hansenispora, etc. (4,(7)(8)(9)(10). H. uvarum, the sporogenous form of Kloeckera apiculata so-called "apiculatus Yeasts" was reported to produce killer toxins (11).…”

mentioning

confidence: 99%

Addition of Some Primary and Secondary Amines to Graphene Oxide, and Studying Their Effect on Increasing its Electrical Properties

Abd¹,

Al-Agha²,

Alheety³

2016

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This study was conducted to isolate and identify killer yeast Hanseniaspora uvarum from dates vinegar and measurement the ability of this yeast to produce killer toxin. The antimicrobial activity of the concentrated supernatant containing partially purified concentrated killer toxin was also detected against several pathogenic bacteria and yeast species, which includes two types of yeast Rhodotorula mucilaginosa and Candida tropicalis and four human pathogenic bacteria Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeurginosa. In addition, the antagonistic activity of examined yeast have been studied toward four types of fungi, where two are pathogenic for human Trichophyton mentagrophytes and Trichophyton rubrum and two are plant pathogens Fusarium solani and Sclerotinia sclerotiorum. The results of killer toxin production experiments revealed the ability of yeast to produce killer toxin with molecular weight at 18 kDa by 12 % SDS electrophoresis. The optimal conditions for killer toxin production were studied, and their antimicrobial activity was determined. The results revealed that killer toxin production was increased at 4 % NaCl, the highest inhibtion zone was 20 mm for S. aureus, while the lowest inhibition zone was 7 mm for E. coli. Killer activity was increased at pH 4 and the best inhibtion zone obtained was about 16 mm for K. pneumoniae, while 8 mm for E. coli and C. tropicalis. The temperature was also affect the production of killer toxin, where 25 °C is the best temperature for toxin production of examined yeast, The best killer activity was 21 mm for C. tropicalis. The antagonistic activity of killer yeast H. uvarum toward pathogenic fungal growth was determined and showed killer activity about 61.11, 44.44, 33.33 and 24.44 % against T. mentagrophytes, T. rubrum, F. solani and S. sclerotiorum in comparison to the control.

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“…However, the ability of killer toxin from D. hansenii DSMZ70238 to kill Staphylococcus aureus, E. coli, Klebsiella pneumoniae, and Streptococcus pyogenes was recently demonstrated by Al-Qaysi et al (Al-Qaysi et al, 2017). Ascomycetous yeast species are especially attractive targets for finding a killer toxin like protein.…”

Section: Gene Expression Of Killer Toxinsmentioning

confidence: 99%

A new report on gene expression of three killer toxin genes with antimicrobial activity of two killer toxins in Iraq

Abu-Mejdad

¹

,

Al-Badran

²

,

Al-Saadoon³

et al. 2020

Bull Natl Res Cent

4

0

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Background The K1, K2, and K28 toxins are usually encoded by several cytoplasmically genetic satellite dsRNAs (M1, M2, and M28), which are encapsulated with virus-like particles (VLPs) and reliant on an additional assembly of assistant yeast viruses (L-A) for their reproduction and encapsidation. Ascomycetous yeast species that have these VLPs are especially attractive targets for finding killer toxins like proteins. This is because the organisms are known in producing a large variety of secondary metabolites and extracellular enzymes, which have medical importance as alternative drugs for resistance bacterial strains, particularly multi-resistance drugs (MRD). Results For the first time, 31 type strains of yeasts were tested for killer toxin production in Iraq via the measurement of gene expression of three killer toxin genes (M1, M2, and M28) within the mycovirus in yeasts. All the type strains gave an expression for the three killer toxins with variable levels. The highest expression was recorded for the killer toxin genes in Torulaspora delbrueckii followed by Wickerhamomyces anomalus. Determined antibacterial activity of two killer toxins appeared with high inhibition zone against pathogenic strains of bacteria. Cytotoxicity against human blood cells was not found. These results considered the first record of killer toxins isolated from type strains in Iraq. Conclusion The two typical strains Torulaspora delbrueckii and Wickerhamomyces anomalus showed the highest level of gene expression for the three killer toxins.

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“…Moreover, in some cases, a killer toxin can interfere with cell wall synthesis of sensitive cells by inhibiting ß-1, 3glucan synthase or by hydrolysis of the major cell wall component ß-1, 3-glucans, mannans, chitin, etc. by interacting with receptors in the cell wall and cytoplasmic membrane.Finally, ion leakage occurs due to the formation of channels in the cytoplasmic membrane and blocking of calcium uptake (4,5,6). The capability of killer toxins production can confer an advantage over more sensitive competitive strains growing in a fermentative process.…”

mentioning

confidence: 99%

“…More than 11 different killer toxins have been studied and identified, many types of yeasts defined as killer toxins such genera as Saccharomyces, Williopsis, Debryomyces, Pichia, Torulaspora, Ustilago, Hansenispora, etc. (4,(7)(8)(9)(10). H. uvarum, the sporogenous form of Kloeckera apiculata so-called "apiculatus Yeasts" was reported to produce killer toxins (11).…”

mentioning

confidence: 99%

Untitled

2019

Baghdad Sci.J

0

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This study was conducted to isolate and identify killer yeast Hanseniaspora uvarum from dates vinegar and measurement the ability of this yeast to produce killer toxin. The antimicrobial activity of the concentrated supernatant containing partially purified concentrated killer toxin was also detected against several pathogenic bacteria and yeast species, which includes two types of yeast Rhodotorula mucilaginosa and Candida tropicalis and four human pathogenic bacteria Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeurginosa. In addition, the antagonistic activity of examined yeast have been studied toward four types of fungi, where two are pathogenic for human Trichophyton mentagrophytes and Trichophyton rubrum and two are plant pathogens Fusarium solani and Sclerotinia sclerotiorum. The results of killer toxin production experiments revealed the ability of yeast to produce killer toxin with molecular weight at 18 kDa by 12 % SDS electrophoresis. The optimal conditions for killer toxin production were studied, and their antimicrobial activity was determined. The results revealed that killer toxin production was increased at 4 % NaCl, the highest inhibtion zone was 20 mm for S. aureus, while the lowest inhibition zone was 7 mm for E. coli. Killer activity was increased at pH 4 and the best inhibtion zone obtained was about 16 mm for K. pneumoniae, while 8 mm for E. coli and C. tropicalis. The temperature was also affect the production of killer toxin, where 25 °C is the best temperature for toxin production of examined yeast, The best killer activity was 21 mm for C. tropicalis. The antagonistic activity of killer yeast H. uvarum toward pathogenic fungal growth was determined and showed killer activity about 61.11, 44.44, 33.33 and 24.44 % against T. mentagrophytes, T. rubrum, F. solani and S. sclerotiorum in comparison to the control.

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Production, Characterization, and Antimicrobial Activity of Mycocin Produced byDebaryomyces hanseniiDSMZ70238

Cited by 37 publications

References 42 publications

Addition of Some Primary and Secondary Amines to Graphene Oxide, and Studying Their Effect on Increasing its Electrical Properties

Addition of Some Primary and Secondary Amines to Graphene Oxide, and Studying Their Effect on Increasing its Electrical Properties

A new report on gene expression of three killer toxin genes with antimicrobial activity of two killer toxins in Iraq

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