This work was evaluated the antioxidant, antibacterial and cytotoxic activity of Costus speciosus rhizomes methanol extract. The FLC analysis showed the presence of five compounds in the methanol extract of C. speciosus rhizomes. These compounds were Quercetin (5.2mg/ml), Rutin (6.02mg/ml), Luteolin, (18.3mg/ml), Kaempherol, (11.34mg/ml) and Coumarin (1.41mg/ml). The maximum antioxidant activity of the extract was at concentration 1000 μg/ml with free radical scavenging activity approximately 67.5%. It was less than standard ascorbic acid 85.5% and Gallic acid 90% with significant difference (p≤0.05), with no significant difference in comparison with standard TBA 68.5%. The IC50 of extract was 3093μg/ml, while the IC50 of ascorbic acid, Gallic acid and TBA were (277.2, 364.5 and 601.3 μg/ml) respectively. The extract revealed influential growth inhibition for all bacteria used in this experiment. The extract was moderately effective at concentration 400 μg/ml of extract with inhibitory activity 50.7 % on MCF-7 cell line and IC50 139.1 μg/ml.
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ISSN: 0067-2904Thabit Iraqi Journal of Science, 2018, Vol. 59, No.1A, pp: 38-43
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IntroductionFrom early years, there is a virtual increasing interest in formation of drugs derived from herbes which can be used as alternative therapy. Also herbal products are safer, less expensive and infrequently have side effects in comparison with synthetic drugs [1]. Among various herbals valued, C. speciosus is an erect herbaceous pharmacologically important one [2]. Costus speciosus is pharmaceutical and decorative plant cultivated in India crepe ginger belongs to family Costaceae (Zingiberaceae) [3]. Costus speciosus have traditional uses such as food and medicine [4]. Recently the juice of rhizome therapeutically implemented as cooling and relief from head-ache, powder of leaves implemented as antipyretic, the extract acquired by boiling stem part of this plant is used against dysentery and fever, and many cures against diarrhea [5] cough, cuts, burning sensation, scabies, arthritis [6], wounds, constipation, leprosy, for abortion, asthma, inflammations, anemia [7] intestinal pains, nose pain , rash, worm infection, to stop vomiting [8] spermatorrhoea [9] used as antivermin and skin diseases [10]. Earlier studies paid attention on antioxidant, cytotoxicity and antimicrobial effects of plant extracts. On the other hand the diagnosis of compounds also had extensive studies to estimate the effect of these compounds [11]. Hence the aimes of the present study were to determine in vitro antioxidant, antibacterial and cytotoxic activity of phenolic compounds in methanol extract of C. speciosus rhizomes.
Materials and methods
Plant materialThe rhizome of C. speciosus was obtained from herbalists market in Baghdad region and was identified by the Faculty of Agriculture, University of Baghdad.
Plant extractionThe sundried rhizomes were powdered with grinder and then passed through sieve with 40 meshes. About 25g of dried rhizomes powder subjected to Soxhlet apparatus and...
