Production and Optimization of Lipase by Aspergillus niger using Coconut Pulp Waste in Solid State Fermentation

Abstract

Lipase has high economical value and an attribute that treasures wide range applications. Among the microbes, fungi to be the best source for lipase production in agro-industrial residue using solid state fermentation (SSF). One of agro-industrial residue is coconut pulp waste. The aims of this study is to explore the possibility of coconut pulp waste for producing of lipase using fungi isolate, Aspergillus niger and find the partial optimum condition of lipase production based on 2 parameters only. The crude enzyme was extracted by mixing fermented substrate with 50 mL sterile aquades pH 7 containing 0.01% Tween-80. The protein concentrations are determined by Bradford method using spectrophotometric. Lipase activity was followed the titrimetric method. The SSF was carried out with variation of initial spore concentration of 1×105, 1×106, 1×107, and 1×108 (spores.mL−1) and crude enzyme was harvested by adding 25, 50, 75, 100, and 125 (mL) of extract solution. The result showed that initial spore concentration and extract solution affected of lipase production. The SSF in coconut pulp waste using A. niger has successfully produced lipase with optimum condition were achieved by adding spore concentration inoculum 1×106 spore.mL−1 in acidic condition at 30°C for 7 days and extracted by 75 mL of extract solution that was result 72.5 mL of crude enzyme with lipase activity 10.83 U.mL−1 and protein content 0.074 mg/mL−1.

Optimization of bioprocess steps through response surface methodology for the production of immobilized lipase using Chaetomium globosum via solid-state fermentation

“…Optimal inoculum size has a significant role in the fermentation process; meanwhile, the aggregation of spores can retard the progression and development of the microorganism [29]. Our findings show similarity with Suyanto et al [30], who reported that the optimum lipase activity for Aspergillus niger was obtained at a spore concentration of 1 × 10 6 spore mL −1 . Previous studies also demonstrated that 83.33% lipase activity was obtained by 1% (v/v) inoculum in addition to the production medium.…”

Characterization of Detergent-Compatible Lipases from Candida albicans and Acremonium sclerotigenum under Solid-State Fermentation

“…This approach provides a stable environment for the growth and production of lipases by fungi . The SSF utilizing Aspergillus niger yielded crude lipase with an activity of 10.83 U/mL . The production of fungal lipase is significantly influenced by the nitrogen source.…”

“… 34 The SSF utilizing Aspergillus niger yielded crude lipase with an activity of 10.83 U/mL. 35 The production of fungal lipase is significantly influenced by the nitrogen source. Wheat bran comprises 13.1 ± 1.3% protein on average, and several authors have reported it as a protein-rich solid substrate for the production of fungal lipase.…”

Evaluation and Characterization of Lipase Production in Aspergillus niger Isolate Qurna for Biotechnological Applications

“…This fungus is a perfect candidate for largescale enzyme production because it can flourish in a variety of environments [36]. The efficient and economical production of lipase for use in various biotechnological and industrial processes can be facilitated by utilizing the enzymatic potential of Aspergillus niger [37].…”