Production and Optimization of Lipase Enzyme from Mesophiles and Thermophiles

Jaiswal, Arvind Kumar; Preet, M; Tripti, B

doi:10.4172/1948-5948.1000355

Cited by 12 publications

(9 citation statements)

References 13 publications

(13 reference statements)

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“…The recorded decline in the activity of lipase after reaching the peak is likely due to attenuation of nutrients in the production medium which led to slowed cell metabolism and the inactivation of the enzyme (Flores et al, 1997). Our results are in consonance with those reported for lipase by Jaiswal et al (2017).…”

Section: Discussionsupporting

confidence: 94%

“…is 7 and 8 respectively. The optimum pH established in this study for both isolates is in harmony with those reported by Jaiswal et al (2017) for lipase. Enzymes that have vast activity at different pH ranges are treasured in the biotechnological industries (Singhal et al 2012).…”

Section: Discussionsupporting

confidence: 88%

“…Lipases, classified as EC 3.1.1.3 are enzymes that catalyze the complete breakdown of oils and fats by converting them into glycerol and fatty acids (Jaiswal et al, 2017). The principal substrate for lipase enzymes are triacylglycerols.…”

Section: Introductionmentioning

confidence: 99%

“…in the diary and food industries (Franken et al, 2009). Lipases are also utilized to augment the absorbency of fabrics in the textile industry, as additives for the production of washing powder in the detergent industries, for diverse transesterification reactions and production of polymers that are biodegradable (Hasan et al, 2006;Jaiswal et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, lipases have also been employed as catalyst for enhanced production of diverse products utilized in paper, pulp and cosmetic industry (Jaiswal et al, 2017). Many thermostable bacterial lipases have been isolated and characterized (Kumar et al, 2005) from different ecological zones.…”

Section: Introductionmentioning

confidence: 99%

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Production and Characterization of Lipase Enzyme Expressed by Crude Oil Contaminated Soil Isolates

Elemuo¹,

Ikiensikimama²,

Ubani³

et al. 2019

ujmr

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Lipases are hydrolytic enzymes responsible for the hydrolysis of triacylglycerol into glycerol and free fatty acids. The biodiversity of crude oil contaminated soil was explored for the isolation of novel potent lipase producing microorganisms. In this current study, microorganisms isolated from crude oil contaminated soils were screened for lipase activity and expression. Six fungal strains namely: Yeast, Aspergillus flavus, Aspergillus niger, Verticillus sp., Penicillum sp., and Microsporum audouini demonstrated lipase producing potentials and the best two: Verticillus sp. and Penicillum sp. were selected for enzyme production and characterization. The lipase enzyme was produced in broth medium. Optimization of selected biochemical parameters reveal that optimum temperature for lipase activity was 40 ºC, pH 7 with an incubation time of 168 h for lipase expressed by Verticillus sp. and 40 ºC, pH 8 with an incubation time of 144 h was established for lipase expressed by Penicillium sp. The kinetic parameters of the lipase enzyme revealed that the enzymes had low Km (0.19 and 0.30 respectively) for the selected substrate.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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