Alkaline lipase production by novel meso-tolerant psychrophilic Exiguobacterium sp. strain (AMBL-20) isolated from glacier of northeastern Pakistan

Yasin, Muhammad Talha; Ali, Yasir; Ahmad, Khurshid; Ghani, Aminuddin Ab; Amanat, Kinza; Basheir, Muhammad Mudassir; Faheem, Muhammad; Hussain, Saddam; Ahmad, Bashir; Hussain, Adil; Bokhari, Syed Ali Imran

doi:10.1007/s00203-020-02133-1

Cited by 14 publications

(3 citation statements)

References 71 publications

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“…They can be obtained principally from several yeasts, fungi and bacteria. Majority of enzymes obtained from bacteria and fungi are employed for industrial purposes (Reddy et al 2003;Plaza-Vinuesa et al 2019;Yasin et al 2021). The amylases are classified into three major groups i.e.…”

Section: Introductionmentioning

confidence: 99%

Enhanced starch hydrolysis by α-amylase using copper oxide nanowires

Ahmad

Khan²,

Yasin

et al. 2021

Appl Nanosci

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α-amylase is the key digestive enzyme that has been used widely in food, paper, detergent and textile industries for starch degradation. This study was conducted for the optimization and characterization of α-amylase production from Aspergillus niger SAIB-4. The study further assessed the effect of metal nanowires (NWs) on starch hydrolysis by α-amylase enzyme from A. niger. Copper oxide (CuO) and iron oxide (FeO) NWs were fabricated in anodized aluminum oxide (AAO) templates. Scanning Electron Microscopy (SEM) and Energy Dispersive spectroscopy (EDS) confirmed the diameter and composition of NWs. Different culture conditions were optimized for the production of α-amylase , where optimum production was obtained at incubation time of 60 h, 5% inoculum size, 1% of banana peel, 0.5% ammonium chloride, pH 8 and 30 °C temperature. It was observed that CuO NWs significantly enhanced α-amylase activity at 40 ppm whereas inhibitory affect was observed for FeO NWs at all concentrations. Maximum starch hydrolysis (0.54 µg/ml) was noticed for CuO NWs at 10 ppm concentration while Minimum activity (0.19 µg/ml) was observed at 70 ppm for FeO NWs. Further, molecular docking analysis was performed to endorse the interaction of NWs with α-amylase with improved enzymatic activity for starch hydrolysis. The combination of α-amylase and CuO NWs could be used as better and efficient source for starch hydrolysis with extraordinary industrial applications, particularly in the sugar and detergent industries.

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Section: Introductionmentioning

confidence: 99%

Enhanced starch hydrolysis by α-amylase using copper oxide nanowires

Ahmad

Khan²,

Yasin

et al. 2021

Appl Nanosci

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“…A number of E. oxidotolerans T-2-2 T strains, like STR36, AMBL-20 T, SGAA-2, A011, N4-1p, CNU020, Kopri 20,246, and isolate 2, have been isolated from variety of the habitats like rhizosphere, glaciers, corals, sea mud and sediments, dead brown algae, and shrimps. These strains played a major role in enzyme production, such as exopolysaccharide and alkaline lipase production, and showed antimicrobial activity and Bgl1C (β-glucosidase), catalase, and protease activity, and were useful for industrial applications (Lee et al 2005;Chen et al 2010;Bharti et al 2013;Cai et al 2014;Chellaram et al 2015;Seki et al 2017;Yasin et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of a pathogenic bacterium, Exiguobacterium oxidotolerans XP-2, from the abnormal diseased mature sporophytes of a commercially cultivated brown seaweed Saccharina japonica

Chen

Zhuang

Qiu³

et al. 2021

J Appl Phycol

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Diseases of the commercially cultivated brown seaweed Saccharina japonica have been noticed since the 1960s in China. However, the usually opportunistic nature of the pathogenic bacteria of seaweeds is still a main obstacle for isolating and identifying pathogenic bacteria responsible for causing diseases of the cultivated S. japonica. In this study, a Gram-positive bacterium XP-2 was isolated from the abnormal diseased mature sporophytes of S. japonica. Pathogenicity test indicated that XP-2 could cause green-rotten disease in healthy juvenile sporophytes and significant algal ultrastructural changes were observed after S. japonica was infected by XP-2. The cells shrank, and the thylakoids of the chloroplasts became blurred after 12 h of re-infection. At 24 h slight deformation was observed in the structure of the mitochondria, chloroplasts, and nucleus. There also were many vacuoles in the infected cells. After 31 h of re-infection, mitochondrial structure and thylakoids became more indistinct. SEM and TEM observations indicated that XP-2 was rod-shaped with no flagella. Analysis of the full length 16S rRNA gene sequence and physiological and chemical characteristics indicated that XP-2 was closely related to Exiguobacterium oxidotolerans 12280 T . Based on these results, XP-2 was designated Exiguobacterium oxidotolerans XP-2. The pathogenicity of E. oxidotolerans XP-2 was identified by Koch's Postulate under laboratory conditions. These results enrich the growing list of pathogenic bacteria in commercially cultivated seaweeds and also provide a foundation for investigating the virulence mechanisms of pathogenic bacteria of commercially cultivated seaweeds in the near future.

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“…Various bacterial strains from different environments, such as seawater, glaciers, hydrothermal vents, industrial effluents, sediments, permafrost, and soil, have exhibited the capability of producing lipase enzymes [ 51 ]. The most important bacterial genera that have been investigated for the production of lipases include Bacillus , Pseudomonas burkholderia , Streptomyces , Arthrobacter , and Archomobacter .…”

Section: Sources Of Microbial Lipasesmentioning

confidence: 99%

The Recent Advances in the Utility of Microbial Lipases: A Review

et al. 2023

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Lipases are versatile biocatalysts and are used in different bioconversion reactions. Microbial lipases are currently attracting a great amount of attention due to the rapid advancement of enzyme technology and its practical application in a variety of industrial processes. The current review provides updated information on the different sources of microbial lipases, such as fungi, bacteria, and yeast, their classical and modern purification techniques, including precipitation and chromatographic separation, the immunopurification technique, the reversed micellar system, aqueous two-phase system (ATPS), aqueous two-phase flotation (ATPF), and the use of microbial lipases in different industries, e.g., the food, textile, leather, cosmetics, paper, and detergent industries. Furthermore, the article provides a critical analysis of lipase-producing microbes, distinguished from the previously published reviews, and illustrates the use of lipases in biosensors, biodiesel production, and tea processing, and their role in bioremediation and racemization.

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Alkaline lipase production by novel meso-tolerant psychrophilic Exiguobacterium sp. strain (AMBL-20) isolated from glacier of northeastern Pakistan

Cited by 14 publications

References 71 publications

Enhanced starch hydrolysis by α-amylase using copper oxide nanowires

Enhanced starch hydrolysis by α-amylase using copper oxide nanowires

Isolation and identification of a pathogenic bacterium, Exiguobacterium oxidotolerans XP-2, from the abnormal diseased mature sporophytes of a commercially cultivated brown seaweed Saccharina japonica

The Recent Advances in the Utility of Microbial Lipases: A Review

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