Production and Characterization of Lipase Enzyme Expressed by Crude Oil Contaminated Soil Isolates

Abstract

Lipases are hydrolytic enzymes responsible for the hydrolysis of triacylglycerol into glycerol and free fatty acids. The biodiversity of crude oil contaminated soil was explored for the isolation of novel potent lipase producing microorganisms. In this current study, microorganisms isolated from crude oil contaminated soils were screened for lipase activity and expression. Six fungal strains namely: Yeast, Aspergillus flavus, Aspergillus niger, Verticillus sp., Penicillum sp., and Microsporum audouini demonstrated lipase producing potentials and the best two: Verticillus sp. and Penicillum sp. were selected for enzyme production and characterization. The lipase enzyme was produced in broth medium. Optimization of selected biochemical parameters reveal that optimum temperature for lipase activity was 40 ºC, pH 7 with an incubation time of 168 h for lipase expressed by Verticillus sp. and 40 ºC, pH 8 with an incubation time of 144 h was established for lipase expressed by Penicillium sp. The kinetic parameters of the lipase enzyme revealed that the enzymes had low Km (0.19 and 0.30 respectively) for the selected substrate.

Microbial Population of Soil and Water around Petroleum Depot Suleja, Nigeria, and their Hydrocarbon Utilisation

“…Among the fungi isolated in this study, Penicillium notatum was prominent across all plots sampled. Elemuo et al [25] have reported the versatility of P. notatum in the environment with ability to produce various enzymes, which helped the microorganisms degrade crude oil in contaminated environment.…”

The use of biological catalyst (enzyme) for enhanced oil recovery in Niger Delta

“…The samples collected were centrifuged at 3000 rpm for 10min before using it for enzyme assay analysis [2,15]. Three enzyme solutions belonging to the classes of lipases and amylase investigated in this study are both isolates of fungi [ 15 ], and it is been presented in Table 3 .…”

Links among Microbial Communities, Soil Properties and Functions: Are Fungi the Sole Players in Decomposition of Bio-Based and Biodegradable Plastic?

“…Such hydrolytic enzymes are important for C, N, and P acquisitions and are therefore meaningful indicators for soil functions [ 25 , 26 ]. Lipases are hydrolytic enzymes produced by animals, plants, and microorganisms, which are responsible for the hydrolysis of triacylglycerol into glycerol and free fatty acids [ 27 , 28 ]. Lipases therefore mediate degradation processes of many BBPs [ 10 , 29 ].…”