Probiotic effect of Bacillus subtilis B-2998D, B-3057D, and Bacillus licheniformis B-2999D complex on sheep and lambs

Abstract

Objectives: Probiotics are well documented for their health benefits by developing a balanced intestinal microbiota and boosting immunity. The present study was conducted to determine the effects of a probiotic preparation Enzimsporin TM (consisting of spore-forming bacteria Bacillus subtilis B-2998D, B-3057D, and Bacillus licheniformis B-2999D) on the biochemical, hematological, immunological parameters, intestinal microbiota, and growth dynamics of sheep and lambs. Materials and Methods: Enzimsporin was fed to lambs and sheep at different doses to determine the bacteria’s probiotic effects. Sheep were divided into three groups (six each), which received 0, 1, and 3 gm of Enzimsporin/per head/day, respectively, and two groups of lambs (10 each), who received 0 gm and 1 gm of Enzimsporin/per head/day for 30 days in addition to their regular ration. On day 30, blood samples were collected, followed by the determination of biochemical, hematological, and natural resistance indicators. Fecal samples were examined to determine the intestinal microflora, and animals were weighed daily to determine their growth dynamics. Results: Supplementation of probiotics (Enzimsporin TM ) improved the lambs’ body weight gain by 18.8%. Analysis of the clinical parameters showed improvements in the levels of total protein, globulins, and urea by 5.3%, 10.8%, and 6.2%, respectively, in the blood of probiotic-supplemented lambs. Similarly, an increment in the total protein, albumins, and globulins was observed in the sheep with Enzimsporin TM supplementation. The decrease in bilirubin and cholesterol levels in the blood and increased bactericidal and phagocytic index in the sheep and lambs with probiotic supplementation indicated a positive influence of Enzimsporin TM on the liver function and natural resistance. Furthermore, an increase in Lactobacillus and Bifidobacterium and a decrease in the Escherichia coli , Enterococcus , and Yeast in the fecal contents of experimental sheep and lambs indicated the potentiality of Enzimsporin TM on maintaining good gut health. Conclusion: Spore-forming bacteria B. subtilis B-2998D, B-3057D, and B. licheniformis B-2999D can be used in feeding sheep and lambs of 2 months of age to increase body weight gain, improve intestinal microbiota, strengthen the immune system, and maintain normal metabolic processes.

Impact of Probiotics on Dairy Production Efficiency

“…Additionally, probiotic supplement improved the growth hormone/insulin-like grows factor-I ratio that enhanced body weights of growth delayed calves. Comparable observations are also recorded in sheep and lambs by Devyatkin et al (2021) . They found that supplementation of a commercial probiotics preparation Enzimsporin (consisting of Bacillus subtilis B-2998D, B-3057D, and Bacillus licheniformis B-2999D) increased body weight gain of sheep and lambs.…”

Effects of inoculation with active microorganisms derived from adult goats on growth performance, gut microbiota and serum metabolome in newborn lambs

“…It is noteworthy that an increase of ALP, CHOL, and HDL was observed in the RF group. A greater concentration of ALP was observed in newborn infants experiencing active bone growth ( Devyatkin et al, 2021 ). Thus, it is plausible to speculate that fresh ruminal fluid inoculation with active microorganisms was beneficial to the development of newborn lambs.…”

Pharmacological effect of T-NilPlus® and Synertox®, commercial anti-mycotoxins, on broiler chickens in Egypt

“…The current study showed that chickens given Synertox ® exerted non-marked alterations in serum levels of cholesterol, triglycerides. These findings were noted by Devyatkin et al (67) ; Mohebbifar et al, (68) and Santoso et al, (69) who observed that no significant impact of a probiotic supplement on the level of cholesterol, triglycerides in chicken blood also chickens given Bacillus subtilis extract exerted non-marked alterations in serum levels of HDL and LDL. This result is reported by Al-Baadani et al, (70) who observed that no significant impact of Bacillus subtilis supplement on the level of HDL, LDL and triglycerides of treated broilers.…”