Probiotic Characteristics and Antifungal Activity of Lactobacillus plantarum and Its Impact on Fermentation of Italian Ryegrass at Low Moisture

Abstract

The study aimed to investigate probiotic characteristics, and low moisture silage fermentation capability of selected lactic acid bacteria (LAB) isolated from Alfalfa (Medicago sativa L). Morphological and physiological properties, carbohydrates fermentation, enzymes, and organic acids production, anti-fungal activity, antibiotic sensitivity patterns, and probiotic characteristics (acidic and bile salt tolerances, hydrophobicity and aggregations natures) of LAB were examined. 16SrRNA sequencing was carried out to identify isolated strains. The identified strains Lactobacillus plantarum (KCC-37) and Lactobacillus plantarum (KCC-38) showed intense antifungal activity, survival tolerant in acidic and bile salt environments, cell surface and auto aggregations ability, enzymes and organic acids productions. At ensiled condition, KCC-37 and KCC-38 enhanced acidification of Italian ryegrass silages by producing a higher amount of lactic acid, a key acid for indicating silage quality with less extent to acetic acid and succinic acid at low moisture level than non-inoculated silages. Notably, the addition of mixed strains of KCC-37 and KCC-38 more potentially enhanced acidification of silage and organic acid productions than the single-culture inoculation. The overall data suggested that these strains could be used as an additive for improving the quality of the fermentation process in low moisture silage with significant probiotic characteristics.

Positive metabolic effects of selected probiotic bacteria on diet‐induced obesity in mice are associated with improvement of dysbiotic gut microbiota

“…Some characteristics have been considered for probiotic selection that includes surviving in the foodstuff at a high cell density and viable in stable throughout the shelf life of a product, acid, bile salt, simulated gastric or intestinal transit, adhere and colonize epithelial cells, inhibition of pathogens and prebiotic-utilizing capability. 28,33,42,60 In this aspect, we analyzed the above criteria for A29 in an in vitro model. The results indicated that A29 survived in significant numbers in simulated gastric, duodenal, and intestinal juices in a time-dependent manner.…”

“…8 Recent reports claimed that the use of the probiotics and or prebiotics could efficiently reduce serum/ plasma cholesterol level and improved overall lipid profiles. 27 Especially, lactobacillus could be considered as potential probiotic strains [28][29][30] and exhibits strong hypocholesterolemic effects in in vivo. 20,26 Lactobacillus not only influences its effect on cholesterol and triglyceride metabolism, but also sustains the gut-associated microbiota.…”

Criteria for lactic acid bacteria screening to enhance silage quality

“…These authors suggested that inoculation may influence the digestibility of DM because of a probiotic effect on the rumen microbial population. This possible probiotic effect has become the focus of studies and the screening of strains as potential inoculants for silages (Ellis et al 2016;Rabelo et al 2017;Srigopalram et al 2017;Soundharrajan et al 2019;Muthusamy et al 2020). Strains that improve quality and reduce losses in silage as well as have a possible probiotic effect on animals have been reported by Ellis et al (2016), Soundharrajan et al (2019) and Muthusamy et al (2020).…”

“…This possible probiotic effect has become the focus of studies and the screening of strains as potential inoculants for silages (Ellis et al 2016;Rabelo et al 2017;Srigopalram et al 2017;Soundharrajan et al 2019;Muthusamy et al 2020). Strains that improve quality and reduce losses in silage as well as have a possible probiotic effect on animals have been reported by Ellis et al (2016), Soundharrajan et al (2019) and Muthusamy et al (2020). Strains are tested for properties such as autoaggregation, coaggregation, hydrophobicity and bile resistance (Srigopalram et al 2017;Soundharrajan et al 2019;Muthusamy et al 2020).…”

“…Strains that improve quality and reduce losses in silage as well as have a possible probiotic effect on animals have been reported by Ellis et al (2016), Soundharrajan et al (2019) and Muthusamy et al (2020). Strains are tested for properties such as autoaggregation, coaggregation, hydrophobicity and bile resistance (Srigopalram et al 2017;Soundharrajan et al 2019;Muthusamy et al 2020). In vitro evaluations using rumen fluid as described by Contreras-Govea et al (2011) are also an excellent option to assess the possible probiotic capacity of strains during selection for silage.…”