Probing small unilamellar EggPC vesicles on mica surface by atomic force microscopy

Force measurements with the atomic force microscope: Technique, interpretation and applications

“…Such experiments give information about the mechanical bending rigidity of the lipid bilayers. For example, Ling et al [792] observed a stiffening of eggPC vesicles when adding cholesterol to the lipid phase.…”

Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques

“…However, artifacts due to interaction with the AFM tip cannot be fully excluded. 42 The depressed center might as well be caused by the tip pushing on a soft lipid island. A detailed picture about the complete fusion process is still unclear.…”

Probing nanomechanical properties from biomolecules to living cells

“…And as a model for the cell membrane, they can be used to assess its global physical properties per se and under the influence of external agencies. In 2004, Liang et al [61] studied unilamellar phosphatidylcholine liposomes by AFM. By fitting the indentation curves to the Hertz model, Young's modulus and the bending modulus were calculated to be about 2 MPa and 0.21×10 −19 J, respectively (details about the significance of bending modulus of lipid bilayers can be found in [61]).…”

“…In 2004, Liang et al [61] studied unilamellar phosphatidylcholine liposomes by AFM. By fitting the indentation curves to the Hertz model, Young's modulus and the bending modulus were calculated to be about 2 MPa and 0.21×10 −19 J, respectively (details about the significance of bending modulus of lipid bilayers can be found in [61]). The same authors then studied the influence of cholesterol on the mechanical properties of the phosphatidylcholine liposomes [60].…”