Pro-inflammatory cytokine expression studies of subclinical and clinical endometritis in endometrial tissues of buffaloes

Bhadaniya, A.R.; Prasad, Mahendra; Savsani, H. H.; Kalaria, V. A.; Fefar, D. T.; Mathpati, B. S.; Javia, B. B.

doi:10.1007/s11250-019-01802-8

Cited by 7 publications

(9 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, serum IL-6 and TNF-α concentrations were higher in CE camels compared to those without CE. Moreover, there was a positive correlation between TNF-α and IL-6 since TNF-α enhances the production of other pro-in ammatory cytokines, such as IL-6, and additional TNF-α (Bhadaniya et al 2019;Lu et al 2013). In addition to, TNF-α is involved in the normal physiology of endometrial proliferation and shedding (Lu et al 2013).…”

Section: Discussionmentioning

confidence: 98%

Research on acute phase proteins and inflammatory cytokines biomarkers in dromedary camels (Camelus dromedarius) with clinical endometritis

El-Deeb

Abdel-Ghani²,

Alhaider³

et al. 2022

Trop Anim Health Prod

View full text Add to dashboard Cite

Uterine diseases are prevalent in she-camel and lead to economic losses because of decreased fertility.The aim of this study is to look into the expression pattern of acute-phase proteins (APPs) and in ammatory cytokines in dromedary camels with clinical endometritis (CE) to highlight their role in immune-pathogenesis of the disease. Moreover, to identify the use of these parameters as a complementary tool for CE screening as well as investigate the e cacy of ceftiofur antibiotic. APPs and in ammatory cytokines were estimated in camels with CE. Values of APPs (Hp, SAA, and Fg), proin ammatory (IFN-γ, TNF-α, IL-1α, IL-1β, and IL-6) and ant-in ammatory cytokines (IL-10) were higher in camels with CE than that of healthy control (P<0.05). The highest correlation was observed between HP and IFN-γ (r = 0.73), and IL-1β and IL-6 (r = 0.73), while the lower correlation was observed between Fg and IFN-γ (r = 0.25). Corynebacterium pyogenes and Arcanobacterium pyogenes were the most common pathogens involved in the etiology of CE. All investigated biomarkers demonstrated a high degree of recognition between CE camel and healthy controls (AUC was > 0.90). A higher proportion of camels with CE that were treated with ceftiofur (90%, P<0.0001) scored clinical cure after the rst dose, while 10% required a second dose. In conclusion, CE causes increased APPs and in ammatory cytokines biomarkers indicating a signi cant acute phase response in diseased camels with CE. These changes in biomarkers could be bene cial for understanding immune-pathogenesis of CE in dromedary camels, clinical practice, and basic clinical research.

show abstract

Section: Discussionmentioning

confidence: 98%

Research on acute phase proteins and inflammatory cytokines biomarkers in dromedary camels (Camelus dromedarius) with clinical endometritis

El-Deeb

Abdel-Ghani²,

Alhaider³

et al. 2022

Trop Anim Health Prod

View full text Add to dashboard Cite

show abstract

“…The elevated serum and endometrial TNF-α in SCE buffaloes at W5 and W7 postpartum was in accordance to Kasimanickam et al (2013) and Brodzki et al (2015) in cows, Fischer et al (2010) and Singh (2017) in buffaloes. Levels of TNF-α was considered a key cytokine which involved in adhesion molecules, like E-Selectin and IL-8 expression that play an important role in PMNs recruitment (Bhadaniya et al, 2019;Roach et al, 2002). On the parallel line, serum and endometrial IL-8 levels were promoted in SCE group.…”

Section: Discussionmentioning

confidence: 99%

“…The congruent positive correlation between PMNs% and serum as well as endometrial levels of TNF-α and IL-8 in SCE buffaloes invigorated the permeation of neutrophils into uterus for withdrawal of the pathogenic micro-organisms (Kasimanickam et al, 2004;Warren, 1990). This could be attributed to the observed promotion in TNF-α and subsequently IL-8 that chemoattracts PMNs into the uterus (Patra et al, 2014) and hasten the endometrial infiltration of PMNs (Bhadaniya et al, 2019;Fischer et al, 2010). C-reactive protein is an essential element of innate system defense that regulates the inflammatory response and acts as a safeguard against infection (Sarikaputi et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

“…Impairment of peri-partum immune function is a causal factor for the progress of pp. uterine infections in bovines (Bhadaniya et al, 2019;Ghanem et al, 2002;Patra et al, 2013). Innate immune system is involved in pathogen recognition in bovine endometritis and reproductive failure.…”

Section: Introductionmentioning

confidence: 99%

“…Tumor necrosis factor alpha, multifunctional pro-inflammatory cytokine, secreted by many cells as macrophages, T lymphocytes and ovarian granulosa cells (Price and Sheldon, 2013). Interleukine-8, the primary neutrophil chemokine, is produced by activated leukocytes that enhance the targeted release of neutrophils and T lymphocytes in order to eliminate pathogens in bovine uterus (Bhadaniya et al, 2019;Mossallam et al, 2015). Both TNF-α and IL-8 are essential in uterine immune cascade of SCE buffaloes via their upregulation in the endometrium (Loyi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Subclinical endometritis and postpartum ovarian resumption in respect to TNF-±, IL-8 and CRP in Egyptian buffaloes

Elsayed¹,

El-Azzazi²,

Mahmoud³

et al. 2020

Anim Reprod

View full text Add to dashboard Cite

The study was carried out to find the relation between subclinical endometritis (SCE) and postpartum (pp.) ovarian resumption as well as to evaluate serum and endometrial TNF-α, IL-8 and serum CRP in buffaloes with and without SCE. Thirty-nine pluriparous buffaloes at the 3 rd (W3), 5 th (W5) and 7 th (W7) week pp. were involved in this experiment. The parity of the buffaloes ranged from 4 to 8 with an average 5.8±0.2. Subclinical endometritis was diagnosed by the percentage of polymorphonuclear cells (PMNs) in uterine cytology obtained from endometrial cytobrush at W5 and W7. The cutoff point of PMNs% in buffaloes for SCE was ≥ 6% at W5 or ≥ 4% at W7. According to PMNs%, buffaloes were divided into SCE group (n=27) and non-SCE group (n=12). Ovarian cyclicity was monitored by rectal palpation, ultrasonography and progesterone assay at W3, W5 and W7. Serum and endometrial TNF-α, IL-8 and serum CRP were estimated at W5 and W7. Buffaloes with SCE (55.6%) showed delayed ovarian activity as compared to non-SCE (16.7%) animals (P=0.036). Significant increase in serum cytokines and CRP levels were detected at W5 (P ˂0.05) and W7 (P <0.01) in SCE buffaloes as compared to non-SCE. Endometrial levels of cytokines were significantly (P ˂0.05) elevated in SCE buffaloes. Serum and endometrial cytokines showed significant positive correlation. Furthermore, levels of TNF-α, IL-8 and CRP exhibited significant positive correlation with PMNs%. In conclusion, SCE delayed postpartum ovarian cyclicity in buffaloes. Moreover, TNF-α, IL-8 and CRP assessments could be efficient tools in prediction of SCE in buffaloes.

show abstract

Characterization of immunological, biochemical and inflammatory response of clinical and subclinical endometritis in ewes in the subtropics

Nasreldin

Ali

Abd‐Elhafeez

et al. 2020

Animal Reproduction Science

View full text Add to dashboard Cite

Pro-inflammatory cytokine expression studies of subclinical and clinical endometritis in endometrial tissues of buffaloes

Cited by 7 publications

References 25 publications

Research on acute phase proteins and inflammatory cytokines biomarkers in dromedary camels (Camelus dromedarius) with clinical endometritis

Research on acute phase proteins and inflammatory cytokines biomarkers in dromedary camels (Camelus dromedarius) with clinical endometritis

Subclinical endometritis and postpartum ovarian resumption in respect to TNF-±, IL-8 and CRP in Egyptian buffaloes

Characterization of immunological, biochemical and inflammatory response of clinical and subclinical endometritis in ewes in the subtropics

Contact Info

Product

Resources

About