Research on acute phase proteins and inflammatory cytokines biomarkers in dromedary camels (Camelus dromedarius) with clinical endometritis

Abstract

Uterine diseases are prevalent in she-camel and lead to economic losses because of decreased fertility.The aim of this study is to look into the expression pattern of acute-phase proteins (APPs) and in ammatory cytokines in dromedary camels with clinical endometritis (CE) to highlight their role in immune-pathogenesis of the disease. Moreover, to identify the use of these parameters as a complementary tool for CE screening as well as investigate the e cacy of ceftiofur antibiotic. APPs and in ammatory cytokines were estimated in camels with CE. Values of APPs (Hp, SAA, and Fg), proin ammatory (IFN-γ, TNF-α, IL-1α, IL-1β, and IL-6) and ant-in ammatory cytokines (IL-10) were higher in camels with CE than that of healthy control (P<0.05). The highest correlation was observed between HP and IFN-γ (r = 0.73), and IL-1β and IL-6 (r = 0.73), while the lower correlation was observed between Fg and IFN-γ (r = 0.25). Corynebacterium pyogenes and Arcanobacterium pyogenes were the most common pathogens involved in the etiology of CE. All investigated biomarkers demonstrated a high degree of recognition between CE camel and healthy controls (AUC was > 0.90). A higher proportion of camels with CE that were treated with ceftiofur (90%, P<0.0001) scored clinical cure after the rst dose, while 10% required a second dose. In conclusion, CE causes increased APPs and in ammatory cytokines biomarkers indicating a signi cant acute phase response in diseased camels with CE. These changes in biomarkers could be bene cial for understanding immune-pathogenesis of CE in dromedary camels, clinical practice, and basic clinical research.

Exploring oxidative stress, immunological and metabolic biomarkers in dairy cows with postpartum pyometra

“…Furthermore, a considerable elevation in SOD levels was observed after treatment when compared to both control and diseased cows. Results in this study are consistent with those observed in previous studies noting a decrease in SOD in she‐camels with uterine infection (El‐Deeb et al., 2022b; El‐Deeb, Iacob, et al., 2022). SOD is an antioxidant enzyme that plays a crucial role in the defence against OS (He et al., 2017; Wang et al., 2018).…”

“…Additionally, the decrease in SOD levels may contribute to the development of tissue damage and sepsis in cows with pyometra. Decreased serum levels of SOD have been found to be a reliable indicator of disease progression and treatment response in several studies (El‐Deeb et al., 2022b; El‐Deeb, Iacob, et al., 2022). However, it is essential to consider the limitations and potential confounding factors of SOD levels when interpreting results in clinical practice.…”