“…This considerable increase in the concentration of these inflammatory mediators could be an indicator of uterine response against inflammation in Arabian mares. Our data are in agreement with previous findings in cows [ 50 ], ewes [ 51 ], and mares [ 28 , 29 , 32 ]. It is well documented that ER/Golgi route is the classical pathway for secretion of cytokines/chemokines [ 33 , 52 ].…”
Background
Extracellular vesicles (EVs) are a promising biomarker and play a vital role in cell–cell communication. This study aimed (I) to identify and characterize EVs from low volume uterine lavage (LVL) and serum in mares with endometritis, compared to healthy controls and (II) to measure serum levels of interleukin 6 (IL-6), and prostaglandins (PGF2α and PGE2). Mares were divided into 30 sub-fertile (endometritis) and 20 fertile (controls). Serum and LVL was collected for EV isolation, and determination of serum levels of inflammatory mediators. Characterization and visualization of EVs were done by electron microscopy, dynamic light scattering and flow cytometry.
Results
Serial ultracentrifugation of LVL and use of a commercial kit for serum were strategies for EVs isolation. Mares with endometritis released higher amounts of larger size EVs. The EVs from mares with endometritis differentially expressed CD9 and CD63, compared to controls. Mares suffering from endometritis evoked higher levels of inflammatory mediators.
Conclusions
Thus, EVs could be used for a better understanding the regulatory mechanisms associated with developing endometritis in mares.
“…This considerable increase in the concentration of these inflammatory mediators could be an indicator of uterine response against inflammation in Arabian mares. Our data are in agreement with previous findings in cows [ 50 ], ewes [ 51 ], and mares [ 28 , 29 , 32 ]. It is well documented that ER/Golgi route is the classical pathway for secretion of cytokines/chemokines [ 33 , 52 ].…”
Background
Extracellular vesicles (EVs) are a promising biomarker and play a vital role in cell–cell communication. This study aimed (I) to identify and characterize EVs from low volume uterine lavage (LVL) and serum in mares with endometritis, compared to healthy controls and (II) to measure serum levels of interleukin 6 (IL-6), and prostaglandins (PGF2α and PGE2). Mares were divided into 30 sub-fertile (endometritis) and 20 fertile (controls). Serum and LVL was collected for EV isolation, and determination of serum levels of inflammatory mediators. Characterization and visualization of EVs were done by electron microscopy, dynamic light scattering and flow cytometry.
Results
Serial ultracentrifugation of LVL and use of a commercial kit for serum were strategies for EVs isolation. Mares with endometritis released higher amounts of larger size EVs. The EVs from mares with endometritis differentially expressed CD9 and CD63, compared to controls. Mares suffering from endometritis evoked higher levels of inflammatory mediators.
Conclusions
Thus, EVs could be used for a better understanding the regulatory mechanisms associated with developing endometritis in mares.
“…Besides, the in ammatory cytokines were signi cantly higher in CE of buffaloes as compared to normal uteri (Bhadaniya et al 2019). Comparable to our ndings, Nasreldin et al (2020) concluded that serum pro-in ammatory cytokines IL-6 and TNF-α are diagnostic markers for CE in ewes and serve as a criterion for different in ammatory complications in ewes classi ed as having CE.…”
Uterine diseases are prevalent in she-camel and lead to economic losses because of decreased fertility.The aim of this study is to look into the expression pattern of acute-phase proteins (APPs) and in ammatory cytokines in dromedary camels with clinical endometritis (CE) to highlight their role in immune-pathogenesis of the disease. Moreover, to identify the use of these parameters as a complementary tool for CE screening as well as investigate the e cacy of ceftiofur antibiotic. APPs and in ammatory cytokines were estimated in camels with CE. Values of APPs (Hp, SAA, and Fg), proin ammatory (IFN-γ, TNF-α, IL-1α, IL-1β, and IL-6) and ant-in ammatory cytokines (IL-10) were higher in camels with CE than that of healthy control (P<0.05). The highest correlation was observed between HP and IFN-γ (r = 0.73), and IL-1β and IL-6 (r = 0.73), while the lower correlation was observed between Fg and IFN-γ (r = 0.25). Corynebacterium pyogenes and Arcanobacterium pyogenes were the most common pathogens involved in the etiology of CE. All investigated biomarkers demonstrated a high degree of recognition between CE camel and healthy controls (AUC was > 0.90). A higher proportion of camels with CE that were treated with ceftiofur (90%, P<0.0001) scored clinical cure after the rst dose, while 10% required a second dose. In conclusion, CE causes increased APPs and in ammatory cytokines biomarkers indicating a signi cant acute phase response in diseased camels with CE. These changes in biomarkers could be bene cial for understanding immune-pathogenesis of CE in dromedary camels, clinical practice, and basic clinical research.
“…Furthermore, we did not study different time-points of secreted cytokine and PGs, as well. Our results were in agreement with Nasreldin et al [38], who revealed the association between endometritis (clinical and subclinical) and inflammatory cyrokines in serum. In previous research works that were done in mares, they only investigated the expression of these mediators in uterine biopsy or their level in cell culture supernatant [7,8,12].…”
Section: Discussionsupporting
confidence: 94%
“…It is well-known that IL-6 is considered as the most important pro-inflammatory cytokine through the inflammation cascade. In addition, IL-6 has a supporting and modulatory role during the inflammation of the tissues, where IL-6 stimulates and potentiates the immune response as well as the action of the other cytokines as IL-10 [38,39]. The IL-6 is a soluble mediator with a pleiotropic effect on inflammation, immune response, and hematopoiesis, whereas it triggers synthesis of acute phase proteins (CRP, serum amyloid A, etc.)…”
So far the intimate link between serum microRNA (miRNA) and uterine inflammation in mares is unknown. We aimed (I) to investigate expression profile of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 (II) and to measure concentrations of interleukin 6 (IL-6), and prostaglandins (PGF2α and PGE2) in serum of mares with healthy and abnormal uterine status (endometritis). This study was conducted on 80 Arabian mares: young (4–7 years), and old (8–14 years). Mares were divided into 48 sub-fertile (endometritis) and 32 fertile (control) at stud farms. Serum was collected for measuring IL-6, PGF2α, and PGE2, as well as miRNA isolation and qRT-PCR. Concentrations of IL-6, PGE2, and PGF2α were higher in mares with endometritis compared to control. Age of mares had a remarkable effect on IL-6, PGE2, and PGF2α concentrations. Relative abundance of eca-miR-155, eca-miR-223, eca-miR-17, eca-miR-200a, and eca-miR-205 was higher in both young and old mares with endometritis. We noticed that eca-miR-155, eca-miR-223, eca-miR-200a, and eca-miR-205 revealed higher expression level in old than young mares with endometritis. This is the first study that has revealed the changes in cell free miRNA and serum inflammatory mediators during endometritis, and these findings could be used for a better understanding the pathophysiology mechanisms of endometritis in equine.
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