Pro-angiogenic effect of PC-3 exosomes in endothelial cells in vitro

Prigol, Anne Natalie; Rode, Michele Patrícia; Silva, Adny Henrique; Cisilotto, Júlia; Creczynski-Pasa, Tânia Beatriz

doi:10.1016/j.cellsig.2021.110126

Cited by 13 publications

(4 citation statements)

References 89 publications

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“…Moreover, αvβ6 integrins in PCa-derived sEVs can be transferred to endothelial cells, which activates TGFβ1 and, results in the inhibition of STAT1 signaling, thereby promoting angiogenesis [50]. Similarly, sEVs secreted by PCa cells have been shown to enhance the angiogenesis and invasion capacity of human umbilical vein endothelial cells, and the same study also suggests that miR-27a-3p may be involved in this phenotypic change [51]. The active cathepsin B protein, which is carried by sEVs can be taken up by endothelial cells by mediating AKT axis phosphorylation, thereby increasing the expression of VEGF and promoting angiogenesis in BC [52].…”

Section: Sevs Promotes Angiogenesis In Urological Tumorsmentioning

confidence: 83%

Current Status of Research on Small Extracellular Vesicles for the Diagnosis and Treatment of Urological Tumors

Zhang

Wang

et al. 2022

Cancers

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Extracellular vesicles (EVs) are important mediators of communication between tumor cells and normal cells. These vesicles are rich in a variety of contents such as RNA, DNA, and proteins, and can be involved in angiogenesis, epithelial-mesenchymal transition, the formation of pre-metastatic ecological niches, and the regulation of the tumor microenvironment. Small extracellular vesicles (sEVs) are a type of EVs. Currently, the main treatments for urological tumors are surgery, radiotherapy, and targeted therapy. However, urological tumors are difficult to diagnose and treat due to their high metastatic rate, tendency to develop drug resistance, and the low sensitivity of liquid biopsies. Numerous studies have shown that sEVs offer novel therapeutic options for tumor treatment, such as tumor vaccines and tumor drug carriers. sEVs have attracted a great deal of attention owing to their contribution to in intercellular communication, and as novel biomarkers, and role in the treatment of urological tumors. This article reviews the research and applications of sEVs in the diagnosis and treatment of urological tumors.

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Section: Sevs Promotes Angiogenesis In Urological Tumorsmentioning

confidence: 83%

Current Status of Research on Small Extracellular Vesicles for the Diagnosis and Treatment of Urological Tumors

Zhang

Wang

et al. 2022

Cancers

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“…Their simultaneous knockdown significantly boosted the maximum toxic effect of DTX in human LnCaP cells, which are considered a good model for the early stages of androgen-dependent prostate cancer [52]. In contrast, no such effect was observed in human PC3 cells, a model for more advanced castration-resistant prostate cancer [53]. Moreover, this increased toxicity occurred without a significant change in the DTX EC50.…”

Section: Discussionmentioning

confidence: 98%

A β-Cyclodextrin-Based Nanoparticle with Very High Transfection Efficiency Unveils siRNA-Activated TLR3 Responses in Human Prostate Cancer Cells

et al. 2022

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Synthetic double-stranded small interfering RNAs (siRNAs) mimic interference RNAs (RNAi) and can bind target mRNAs with a high degree of specificity, leading to selective knockdown of the proteins they encode. However, siRNAs are very labile and must be both protected and transported by nanoparticles to be efficiently delivered into cells. In this work, we used a Janus-type polycationic amphiphilic β-cyclodextrin derivative to efficiently transfect siRNAs targeting mRNAs encoding mitogen-activated protein kinase (p42-MAPK) or Ras homolog enriched in brain (Rheb) into different cancer cell lines as well as astrocytes. We took advantage of this high transfection efficiency to simultaneously knock down p42-MAPK and Rheb to boost docetaxel (DTX)-mediated toxicity in two human prostate cancer cell lines (LNCaP and PC3). We found that double knockdown of p42-MAPK and Rheb increased DTX-toxicity in LNCaP but not in PC3 cells. However, we also observed the same effect when scramble siRNA was used, therefore pointing to an off-target effect. Indeed, we found that the siRNA we used in this work induced toll-like receptor 3 activation, leading to β-interferon production and caspase activation. We believe that this mechanism could be very useful as a general strategy to elicit an immune response against prostate cancer cells.

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“…Since angiogenesis is integral to tumor cell survival, sEVs-miRNAs have been identified as key contributors to angiogenesis in urological tumors. For instance, a study by Prigol et al (2021) indicated that sEVs from PC-3 cells prompted angiogenic behavior in HUVECs, a finding attributable to the overexpression of miR-27a-3p in PC-3 sEVs. This observation suggests the potential involvement of miR-27a-3p in pro-angiogenic effects.…”

Section: The Role Of Angiogenesis In Urological Tumorsmentioning

confidence: 99%

Role of microRNA carried by small extracellular vesicles in urological tumors

Mao

Zhang

Wang

et al. 2023

Front. Cell Dev. Biol.

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Small extracellular vesicles (sEVs) are minute vesicles secreted by various cells that are capable of transporting cargo, including microRNAs, between donor and recipient cells. MicroRNAs (miRNAs), small non-coding RNAs approximately 22 nucleotides in length, have been implicated in a wide array of biological processes, including those involved in tumorigenesis. Emerging evidence highlights the pivotal role of miRNAs encapsulated in sEVs in both the diagnosis and treatment of urological tumors, with potential implications in epithelial-mesenchymal transition, proliferation, metastasis, angiogenesis, tumor microenvironment and drug resistance. This review provides a brief overview of the biogenesis and functional mechanisms of sEVs and miRNAs, followed by a summarization of recent empirical findings on miRNAs encapsulated in sEVs from three archetypal urologic malignancies: prostate cancer, clear cell renal cell carcinoma, and bladder cancer. We conclude by underscoring the potential of sEV-enclosed miRNAs as both biomarkers and therapeutic targets, with a particular focus on their detection and analysis in biological fluids such as urine, plasma, and serum.

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Pro-angiogenic effect of PC-3 exosomes in endothelial cells in vitro

Cited by 13 publications

References 89 publications

Current Status of Research on Small Extracellular Vesicles for the Diagnosis and Treatment of Urological Tumors

Current Status of Research on Small Extracellular Vesicles for the Diagnosis and Treatment of Urological Tumors

A β-Cyclodextrin-Based Nanoparticle with Very High Transfection Efficiency Unveils siRNA-Activated TLR3 Responses in Human Prostate Cancer Cells

Role of microRNA carried by small extracellular vesicles in urological tumors

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