Printing via Photolithography on Micropartitioned Fluid Lipid Membranes

Abstract

A four‐level grayscale image of Abraham Lincoln on a lipid bilayer membrane(see Figure) is used to demonstrate this novel single‐exposure photolithographic procedure for printing images on fluid surfaces containing partitioned corrals, the very nature of the fluid membrane allowing true grayscale to be achieved. The potential scope and applications—particularly in biotechnology—of the concept are also discussed.

Solid supported lipid bilayers: From biophysical studies to sensor design

“…The bilayers retained two-dimensional fluidity within a given corral, but the barriers did not allow mixing between neighboring patches as was demonstrated by fluorescence microscopy [13,130]. Limited differentiation between the bilayers could be achieved by selective photobleaching [131]. Fig.…”

Arrays of lipid bilayers and liposomes on patterned polyelectrolyte templates

“…Arrays of BLMs have also been fabricated on glass and gold surfaces [26,33,[41][42][43][44][45][46][47][48]. In one approach, a patterned poly(dimethyl siloxane) (PDMS) stamp was brought into contact with an sBLM formed on a glass slide and then removed [41].…”

Micropattern Formation in Supported Lipid Membranes

“…In the case of an array of isolated membrane corrals, for example, different lipid compositions or proteins can be deposited or flowed into individual corrals in the array, thus producing a mosaic pattern of fluid membrane elements, each with differing compositions. 34,36,37 Such membrane microarrays are proving useful in membrane discrimination studies 38 and multiplexed screening applications.…”