Arrays of lipid bilayers and liposomes on patterned polyelectrolyte templates

Kohli, Neeraj; Vaidya, Sachin S.; Ofoli, Robert Y.; Worden, R. Mark; Lee, Ilsoon

doi:10.1016/j.jcis.2006.05.048

Cited by 27 publications

(26 citation statements)

References 75 publications

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“…The diffusion coefficient was obtained as D = (1.1 ± 0.5) Â 10 À9 cm 2 /s by fitting the data with the commonly used equation [17]. The value is one order of magnitude lower than that of lipid bilayers on glass surfaces [17] but is comparable to the one for the lipid bilayers on a PEM film reported previously (0.21-0.72 Â 10 À9 cm 2 s À1 ), where the one or two orders of magnitudes smaller value of D compared to that on a glass slide has been linked either to the strong coupling between the lipid molecules and the oppositely charged PEM or to the result of insufficient connectivity of the lipid bilayer [18][19][20]. Note that the lipid structures tend to change their appearance when they are heated locally by the laser during the photo-bleaching (see the bright dots that appeared during the recovery in Fig.…”

Section: Resultssupporting

confidence: 80%

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Electrically induced lipid migration in non-lamellar phase

Sugihara

Stucki

Isa

et al. 2012

Journal of Colloid and Interface Science

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Section: Resultssupporting

confidence: 80%

“…It has garnered attention due to its inverted hexagonal phase (H II ) at full hydration and room temperature ( Fig. 1B) [17,18]. In aqueous solution, it forms H II blocks, characterized by electron microscopy [11,12], including our previous work [13].…”

Section: Introductionmentioning

confidence: 90%

Electrically induced lipid migration in non-lamellar phase

Sugihara

Stucki

Isa

et al. 2012

Journal of Colloid and Interface Science

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“…We performed FRAP experiments where lipid diffusion and fluorescence recovery on the surface of the colloid were followed over lateral separations of several micrometres. [36][37][38] Diffusive fluorescence recovery over these comparatively large distances thus not only requires lipid mobility, but also connectivity of the lipid film on the scale of 1 Â 10 1 mm. Fig.…”

Section: Lipid Diffusion and Layer Connectivitymentioning

confidence: 99%

Lipid layers on polyelectrolyte multilayer supports

et al. 2008

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The mechanism of formation of supported lipid layers from phosphatidylcholine and phosphatidylserine vesicles in solution on polyelectrolyte multilayers was studied by a variety of experimental techniques. The interaction of zwitterionic and acidic lipid vesicles, as well as their mixtures, with polyelectrolyte supports was followed in real time by micro-gravimetry. The fabricated lipid-polyelectrolyte composite structures on top of multilayer coated colloidal particles were characterized by flow cytometry and imaging techniques. Lipid diffusion over the macroscopic scale was quantified by fluorescence recovery after photobleaching, and the diffusion was related to layer connectivity. The phospholipid-polyelectrolyte binding mechanism was investigated by infrared spectroscopy. A strong interaction of polyelectrolyte primary amino groups with phosphate and carboxyl groups of the phospholipids, leading to dehydration, was observed. Long-range electrostatic attraction was proven to be essential for vesicle spreading and rupture. Fusion of lipid patches into a homogeneous bilayer required lateral mobility of the lipids on the polyelectrolyte support. The binding of amino groups to the phosphate group of the zwitterionic lipids was too weak to induce vesicle spreading, but sufficient for strong adsorption. Only the mixture of phosphatidylcholine and phosphatidylserine resulted in the spontaneous formation of bilayers on polyelectrolyte multilayers. The adsorption of phospholipids onto multilayers displaying quarternary ammonium polymers produced a novel 3D lipid polyelectrolyte structure on colloidal particles.

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“…Substrate-supported lipid bilayers (SLBs) have attracted considerable interest as models of cellular membranes (Castellana and Cremer, 2006;Chan and Boxer, 2007;Daniel et al, 2006;Groves and Dustin, 2003;Groves et al, 1997;Kohli et al, 2006;Lenhert et al, 2007;Ottova and Tien, 2002;Richter et al, 2006;Sackmann, 1996;Stroumpoulis et al, 2007;Tamm and McConnell, 1985;Tanaka and Sackmann, 2006). Proteins reconstituted in SLBs have been shown to retain their native activity while the lipid components remain mobile, due primarily to the thin $1-2 nm water layer that separates the lower leaflet of the bilayer from the solid support (Kiessling and Tamm, 2003;Johnson et al, 1991;Koenig et al, 1996).…”

Section: Introductionmentioning

confidence: 99%