Primer reporte de begomovirus infectando cultivos de ají (&lt;i&gt;Capsicum&lt;/i&gt; spp.) en Colombia

Vaca-Vaca, Juan Carlos; Morales-Euse, Jonathan; Rivera-Toro, Diana Marcela; López‐López, Karina

doi:10.15446/abc.v24n3.79367

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…Only the presence of a potyvirus, the pepper deforming mosaic virus (PepDMV) (Morales et al, 2005) and recently, a begomovirus (Vaca-Vaca et al, 2019) have been reported as presently affecting these crops. The cucumber mosaic virus (CMV) has only been detected using serological techniques like ELISA.…”

Section: Introductionmentioning

confidence: 99%

Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops

2020

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The chili pepper (Capsicum spp. L.) is a vegetable of economic importance that has been affected worldwide by the cucumber mosaic virus (CMV), a pathogen that causes a devastating disease in this crop. The aim of this research was the detection and characterization of CMV in chili pepper crops in Valle del Cauca, Colombia. Leaves of three chili pepper varieties (tabasco, cayenne and habanero) with viral symptoms were collected in four municipalities of Valle del Cauca. Total RNA was purified and a fragment of capsid protein (CP) from CMV was amplified by RT‑PCR. Then, it was sequenced and bioinformatically analyzed, and from these sequences, specific primers were designed. From 71 chili pepper samples collected in Palmira, Yumbo, Vijes and Yotoco, 37 were positive for CMV (52.1%). The CMV chili pepper sequence analysis showed that they had their highest identity (98.5%) with a CMV isolated from bananas in Ecuador. Specific primers designed for CMV chili pepper showed greater sensitivity for detecting this virus (64.7% vs. 52.1%). The CMV chili pepper CP analysis indicated that it could be transmitted by the species Aphis gossypii. This r the first time, the molecular characterization of CMV in three chili pepper varieties.

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Section: Introductionmentioning

confidence: 99%

Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops

2020

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“…Recently, a new begomovirus was detected in chili peppers (Capsicum spp.) in Valle del Cauca but its identity is being studied (Vaca-Vaca et al, 2019b).…”

Section: Introductionmentioning

confidence: 99%

Partial molecular characterization of begomoviruses isolated from weeds collected in tomato crops in the southeast of Valle del Cauca, Colombia

Vaca-Vaca¹,

Jara-Tejada²,

López‐López³

2020

Rev. Colomb. Cienc. Hortic.

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Weeds are usually considered as a source of new viruses but are often neglected during diversity studies. Previously eleven samples of weeds were collected along the edges of a tomato crop field located in four municipalities (Florida, Ginebra, Cerrito and Candelaria) in the Southeast of Valle del Cauca. These samples were positive for begomoviruses but their molecular characterization had not been done until now. For each sample, DNA fragments were cloned, sequenced and analyzed. Nucleotide sequence analysis of viral fragments showed the presence of six different begomoviruses: two virus isolated from Lantana camara, Desmodium sp. and Amaranthus dubius were previously described as potato yellow mosaic virus (PYMV) and passionfruit leaf distortion virus (PLDV), respectively; other four virus which were isolated from L. camara, A. dubius, Rivina humilis, Desmodium sp., Rhynchosia minima, Hybanthus attenuatus, Verbena sp., Croton hirtus and Caesalpinia sp., showed its highest nucleotide sequence identity (89%) with bean chlorotic mosaic virus (BChMV), datura leaf distortion virus (DaLDV) and rhynchosia golden mosaic Yucatan virus (RhGMYV). Cloned viruses fragments from these weeds could be new begomoviruses not previously reported before, this in accordance with the ICTV species demarcation criterion for the genus Begomovirus (≥91 % sequence identity). Mixed infections of begomoviruses in Desmodium sp. and A. dubius weeds also was found in this analysis. Finally, this paper reports by the first time to three alternative begomoviruses weeds hosts that infect tomato and passion fruit crops: A. dubius for PYMV, and L. camara and Desmodium sp. for PLDV, respectively.

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First molecular characterization of pepper severe mottle virus infecting chili pepper crops in Colombia

2021

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Primer reporte de begomovirus infectando cultivos de ají (<i>Capsicum</i> spp.) en Colombia

Cited by 4 publications

References 32 publications

Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops

Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops

Partial molecular characterization of begomoviruses isolated from weeds collected in tomato crops in the southeast of Valle del Cauca, Colombia

First molecular characterization of pepper severe mottle virus infecting chili pepper crops in Colombia

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