Primary recovery of lipase derived from Burkholderia cenocepacia strain ST8 and recycling of phase components in an aqueous two-phase system

Show, Pau Loke; Tan, Chin Ping; Anuar, M. S.; Ariff, Arbakariya; Yusof, Yus Aniza; Chen, Soo Kien; Ling, Tau Chuan

doi:10.1016/j.bej.2011.10.005

Cited by 49 publications

(24 citation statements)

References 41 publications

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“…In general, their results showed successful partitioning of the desired product (laccase) towards the top phase (PEG), while the contaminants were partitioned to the bottom phase (phosphate buffer solution) with the highest recovery percentage (up to 60%) with PEG 1000 and PEG 1450. Although studies on the recovery of laccases using ATPS are scarce there are several reports of the recovery of other proteins …”

Section: Resultsmentioning

confidence: 99%

Pleurotus ostreatuslaccase recovery from residual compost using aqueous two-phase systems

Bertrand

Mayolo‐Deloisa

Tinoco‐Valencia

et al. 2016

J. Chem. Technol. Biotechnol.

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BACKGROUND: Multiple aqueous two-phase systems (ATPS) were evaluated for the partial purification of laccase from a crude aqueous extract. The laccase extract was obtained from residual compost, generated from the production of the edible mushroom Pleurotus ostreatus. A total of 59 ATPS were evaluated; 16 systems using poly (ethylene glycol) (PEG) and phosphates, 20 systems using UCON (ethylene oxide/propylene oxide copolymer) and different salts, and 23 polymer-polymer systems (Ficoll, Dextran and PEG).

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Section: Resultsmentioning

confidence: 99%

Pleurotus ostreatuslaccase recovery from residual compost using aqueous two-phase systems

Bertrand

Mayolo‐Deloisa

Tinoco‐Valencia

et al. 2016

J. Chem. Technol. Biotechnol.

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“…The concentrated EOPO solution from the secondary ATPS can be reused to further extract proteins from the salt solution in the primary ATPS (Fig. 2) [40].…”

Section: Novel Lipase Purification Methodsmentioning

confidence: 99%

“… An illustration of an extractive fermentation process using the recycling ATPS developed by Show et al [40] (*Reproduced with permission from Elsevier). …”

Section: Novel Lipase Purification Methodsmentioning

confidence: 99%

Novel lipase purification methods – a review of the latest developments

Tan

Show

Ooi

et al. 2014

Biotechnology Journal

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Microbial lipases are popular biocatalysts due to their ability to catalyse diverse reactions such as hydrolysis, esterification, and acidolysis. Lipases function efficiently on various substrates in aqueous and non-aqueous media. Lipases are chemo-, regio-, and enantio-specific, and are useful in various industries, including those manufacturing food, detergents, and pharmaceuticals. A large number of lipases from fungal and bacterial sources have been isolated and purified to homogeneity. This success is attributed to the development of both conventional and novel purification techniques. This review highlights the use of these techniques in lipase purification, including conventional techniques such as: (i) ammonium sulphate fractionation; (ii) ion-exchange; (iii) gel filtration and affinity chromatography; as well as novel techniques such as (iv) reverse micellar system; (v) membrane processes; (vi) immunopurification; (vi) aqueous two-phase system; and (vii) aqueous two-phase floatation. A summary of the purification schemes for various bacterial and fungal lipases are also provided.

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“…The impact of the loaded mass on the partition of enzymes is important, as the loaded feed stock can alter the phase volume ratio [44] and the partition behavior of target protein [45]. The increasing amounts of both polygalacturonase and contaminants in the systems could result in a decrease of the liquid/liquid extraction process performance.…”

Section: The Effect Of Crude Feedstock Concentration and Total Volumementioning

confidence: 99%

A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from Durio zibethinus

Amid

Manap

Asmadi

et al. 2015

Journal of Chromatography B

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a b s t r a c tPolygalacturonase is one of the important enzymes used in various industries such as food, detergent, pharmaceutical, textile, pulp and paper. A novel liquid/liquid extraction process composed of surfactant and acetonitrile was employed for the first time to purify polygalacturonase from Durio zibethinus. The influences of different parameters such as type and concentration of surfactants, concentrations of acetonitrile and composition of surfactant/acetonitrile on partitioning behavior and recovery of polygalacturonase was investigated. Moreover, the effect of pH of system and crude load on purification fold and yield of purified polygalacturonase were studied. The results of the experiment indicated the polygalacturonase was partitioned into surfactant top rich phase with impurities being partitioned into acetonitrile bottom rich phase in the novel method of liquid/liquid process composed of 23% (w/w) Triton X-100 and 19% (w/w) acetonitrile, at 55.6% of TLL (tie line length) crude load of 25% (w/w) at pH 6.0. Recovery and recycling of components also was measured in each successive step of liquid/liquid extraction process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 97.3% while phase components were also recovered and recycled above 95%. This study demonstrated that the novel method of liquid/liquid extraction process can be used as an efficient and economical extraction method rather than the traditional methods of extraction for the purification and recovery of the valuable enzyme.

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Primary recovery of lipase derived from Burkholderia cenocepacia strain ST8 and recycling of phase components in an aqueous two-phase system

Cited by 49 publications

References 41 publications

Pleurotus ostreatuslaccase recovery from residual compost using aqueous two-phase systems

Pleurotus ostreatuslaccase recovery from residual compost using aqueous two-phase systems

Novel lipase purification methods – a review of the latest developments

A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from Durio zibethinus

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