2016
DOI: 10.1002/jctb.4995
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Pleurotus ostreatuslaccase recovery from residual compost using aqueous two-phase systems

Abstract: BACKGROUND: Multiple aqueous two-phase systems (ATPS) were evaluated for the partial purification of laccase from a crude aqueous extract. The laccase extract was obtained from residual compost, generated from the production of the edible mushroom Pleurotus ostreatus. A total of 59 ATPS were evaluated; 16 systems using poly (ethylene glycol) (PEG) and phosphates, 20 systems using UCON (ethylene oxide/propylene oxide copolymer) and different salts, and 23 polymer-polymer systems (Ficoll, Dextran and PEG).

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Cited by 18 publications
(15 citation statements)
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References 23 publications
(60 reference statements)
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“…A new approach which has recently gained popularity for downstream processing in other biomolecules is Aqueous Two-Phase Systems (ATPS). This strategy has been successful for the recovery and partial purification of enzymes and other micro and macro molecules, but has been hardly used for the treatment of crude Bs extracts [53]. Up to date, the application of ATPS for Bs downstream processing remains basically unexplored in the biotechnological field.…”
Section: Improving Downstream Processesmentioning
confidence: 99%
“…A new approach which has recently gained popularity for downstream processing in other biomolecules is Aqueous Two-Phase Systems (ATPS). This strategy has been successful for the recovery and partial purification of enzymes and other micro and macro molecules, but has been hardly used for the treatment of crude Bs extracts [53]. Up to date, the application of ATPS for Bs downstream processing remains basically unexplored in the biotechnological field.…”
Section: Improving Downstream Processesmentioning
confidence: 99%
“…Important to mention is that until now, protocols for laccase partial purification by the use of ATPS cannot be generalized, as laccase from different sources display contrasting results in terms of yield, and depending on the ATPS system used. Additionally, very little research has been done using this one‐step purification technology for laccase purification …”
Section: Chemical Modification Strategies For Laccase Improvementmentioning
confidence: 99%
“…Additionally, very little research has been done using this one-step purification technology for laccase purification. 156 Table 1C provides a few more examples of chemical modification of laccases, generally used for immobilizing purposes, resulting in enhanced activity and stability.…”
Section: Laccase Immobilization Technologiesmentioning
confidence: 99%
“…Traditionally, ATPS have been extensively used for separation and recovery of proteins . However, ATPS have shown also great potential in the fractionation, recovery and partial purification of other biological products such as low molecular weight compounds and macromolecules, organelles and even whole cells (Fig.…”
Section: Introductionmentioning
confidence: 99%