Primary olfactory mucosal cells promote axonal outgrowth in a three-dimensional assay

Ishihara, Masahiro; Mochizuki-Oda, Noriko; Iwatsuki, Koichi; Kishima, Haruhiko; Ohnishi, Yozo; Moriwaki, Takashi; Umegaki, Masao; Yoshimine, Toshiki

doi:10.1002/jnr.23367

Cited by 11 publications

(12 citation statements)

References 41 publications

(65 reference statements)

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“…Another example of contact mediated increase of axonal outgrowth induced by OECs includes DRG axons grown on myelin substrates [44]. In our study, the secretome of OECs did not reveal to be a growth promoting substrate for axonal outgrowth of DRG explants, even though there is a substantial body of literature supporting the relevance of OECs' secreted factors in axonal regeneration context [45][46][47]. The contact-mediated ability of OECs might be partially due to the action of metalloproteinases (MMPs) such as MMP-2, MMP-3, and MT1-MMP, which seem to play a role on cellular motility and on their neurotrophic properties [48].…”

Section: Discussioncontrasting

confidence: 55%

Co-Transplantation of Adipose Tissue-Derived Stromal Cells and Olfactory Ensheathing Cells for Spinal Cord Injury Repair

et al. 2018

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Patients suffering from spinal cord injury (SCI) still have a dismal prognosis. Despite all the efforts developed in this area, currently there are no effective treatments. Therefore, cell therapies have been proposed as a viable alternative to the current treatments used. Adipose tissue-derived stromal cells (ASCs) and olfactory ensheathing cells (OECs) have been used with promising results in different models of SCI, namely due to the regenerative properties of the secretome of the first, and the guidance capability of the second. Using an in vitro model of axonal growth, the dorsal root ganglia explants, we demonstrated that OECs induce neurite outgrowth mainly through cell-cell interactions, while ASCs' effects are strongly mediated by the release of paracrine factors. A proteomic analysis of ASCs' secretome revealed the presence of proteins involved in VEGF, PI3K, and Cadherin signaling pathways, which may be responsible for the effects observed. Then, the cotransplantation of ASCs and OECs showed to improve motor deficits of SCI-rats. Particular parameters of movement such as stepping, coordination, and toe clearance were improved in rats that received the transplant of cells, in comparison to nontreated rats. A histological analysis of the spinal cord tissues revealed that transplantation of ASCs and OECs had a major effect on the reduction of inflammatory cells close the lesion site. A slight reduction of astrogliosis was also evident. Overall, the results obtained with the present work indicate that the cotransplantation of ASCs and OECs brings important functional benefits to the injured spinal cord. Stem Cells 2018;36:696-708.

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Section: Discussioncontrasting

confidence: 55%

Co-Transplantation of Adipose Tissue-Derived Stromal Cells and Olfactory Ensheathing Cells for Spinal Cord Injury Repair

et al. 2018

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“…GBCs are positive for early neuronal differentiation markers such as Mash1, Neurogenin1, N-CAM, Sox2, and nestin (Gordon et al, 1995;Beites et al, 2005;Thakur et al, 2014), hence confirming that they are neural progenitor cells, while HBCs express keratin 5 and keratin 14 but are negative for neuronal markers (Carter et al, 2004) (see Table 1). However, only specific antibodies GBC-2 and GBC III were used to isolate GBCs from the OE by FACS, according to recent data (Chen et al, 2004;Krolewski et al, 2013;Thakur et al, 2013Thakur et al, , 2014, while ICAM-1 was Brought to you by | New York University Bobst Library Technical Services Authenticated Download Date | 5/25/15 10:27 PM Human Gorrie et al, 2010;Kachramanoglou et al, 2013;Tabakow et al, 2013;Xie et al, 2013 Rat Rizek andKawaja, 2006;Kalincik et al, 2010;Centenaro et al, 2011;Kueh et al, 2011;Paviot et al, 2011;Stamegna et al, 2011;Tharion et al, 2011;Mayeur et al, 2013;Ishihara et al, 2014Dog Granger et al, 2012Ziege et Rat Kalincik et al, 2010;Centenaro et al, 2011;Paviot et al, 2011;Stamegna et al, 2011;Ishihara et al, 2014 S100 OECs Human Gorrie et al, 2010;Kachramanoglou et al, 2013;Tabakow et al, 2013Rat Centenaro et al, 2011 ( used to purify HBCs (Iwai et al, 2008;Fletcher et al, 2011;…”

Section: Gbcs and Hbcs: Characteristics And Potential Therapeutic Valmentioning

confidence: 99%

Olfactory mucosa: a rich source of cell therapy for central nervous system repair

Duan

2015

Reviews in the Neurosciences

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AbstractDamage to the brain and spinal cord leads to permanent functional disability because of the very limited capacity of the central nervous system (CNS) for repair. Cell therapy is thought to be a promising strategy for CNS repair. The proper cell type of transplantation for CNS repair has not been identified until now, but autologous transplantation would be advantageous. The olfactory mucosa (OM), from the olfactory system, in which the neurosensory cells are replaced throughout adult life, is thought to be a rich source of cell therapy for CNS repair. The OM is a heterogeneous tissue composed of a variety of cells supporting both normal function and regenerative capacity, in which many studies focused on four major types of cells, including horizontal basal cells (HBCs), globose basal cells (GBC), mesenchymal stem cells (MSCs), and olfactory ensheathing cells (OECs). Here, we review the four major types of cells in the OM and shed light on the potential of the OM for CNS repair.

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“…Six patients received autologous OEC transplantation in a Phase I clinical trial, of whom three patients showed signs of improvement of SCI, and two improved from ASIA-A to -C and -B (127). To overcome the difficulty to obtain OECs, olfactory mucosa cells (OMCs) were tested and have promoted axonal outgrowth (128, 129). However, OMC transplantation performed in Portugal resulted in formation of tumor-like structure, which could have been due to improper purification of cells (130).…”

Section: Cell-based Therapiesmentioning

confidence: 99%

Drug delivery, cell-based therapies, and tissue engineering approaches for spinal cord injury

Kabu

Gao

Kwon

et al. 2015

Journal of Controlled Release

162

114

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Spinal cord injury (SCI) results in devastating neurological and pathological consequences, causing major dysfunction to the motor, sensory and autonomic systems. The primary traumatic injury to the spinal cord triggers a cascade of acute and chronic degenerative events, leading to further secondary injury. Many therapeutic strategies have been developed to potentially intervene in these progressive neurodegenerative events and minimize secondary damage to the spinal cord. Additionally, significant efforts have been directed towards regenerative therapies that may facilitate neuronal repair and establish connectivity across the injury site. Despite the promise that these approaches have shown in preclinical animal models of SCI, challenges with respect to successful clinical translation still remain. The factors that could have contributed to failure include important biologic and physiologic differences between the preclinical models and the human condition, study designs that do not mirror clinical reality, discrepancies in dosing and the timing of therapeutic interventions, and dose-limiting toxicity. With a better understanding of the pathobiology of events following acute SCI, developing integrated approaches aimed at preventing secondary damage and also facilitating neurodegenerative recovery is possible, and hopefully will lead to effective treatments for this devastating injury. The focus of this review is to highlight the progress that has been made in drug therapies and delivery systems, and also cell-based and tissue engineering approaches for SCI.

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Primary olfactory mucosal cells promote axonal outgrowth in a three-dimensional assay

Cited by 11 publications

References 41 publications

Co-Transplantation of Adipose Tissue-Derived Stromal Cells and Olfactory Ensheathing Cells for Spinal Cord Injury Repair

Co-Transplantation of Adipose Tissue-Derived Stromal Cells and Olfactory Ensheathing Cells for Spinal Cord Injury Repair

Olfactory mucosa: a rich source of cell therapy for central nervous system repair

Drug delivery, cell-based therapies, and tissue engineering approaches for spinal cord injury

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