Primary culture of human esophageal epithelial cells.

Novel S100 Proteins in Human Esophageal Epithelial Cells: CAAF 1 Expression is Associated with Cell Growth Arrest.

“…The primary culture of human esophageal epithelial cells (EECs) was performed by a previously published method (KATAYAMA et al, 1984) modified as follows: Each piece of normal esophageal epithelia was treated with 2.5ag/ml of Fungizone(GIBCO BRL, Life Technologies, Rockville, MD) in Media I (IBL, Takasaki, Japan) containing 5% fetal bovine serum (FBS) (Mitsubishi Kasei Co., Tokyo, Japan) at 4°C overnight, and the subepithelial connective tissue was carefully peeled off the epithelium, which was then minced finely and trypsinized at 37t for 30 min. Following incubation, the action of the trypsin was stopped by the addition of soybean trypsin inhibitor (GIBCO BRL), and the suspension was spun to collect the cells.…”

Establishment and Characterization of an SV40 Large T Antigen-Transduced Porcine Colonic Epithelial Cell Line

“…Consequently, researchers are forced to frequently reestablish cultures, but this is often restricted for ethical and financial reasons [Vos et al, 2012]. Moreover, the standardization of primary cell culture protocols is difficult due to a high variability between individual isolations, rapid dedifferentiation [Hartung et al, 2002], and a high incidence of fibroblastic overgrowth [Barnes and Masui, 1981;Jensen and Therkelsen, 1982;Katayama et al, 1984;Desmarets et al, 2013]. These limitations, along with the need for sufficient and consistent sample materials, have increased the demand for well-characterized and continuously growing cell lines, particularly in studies of epithelial barrier function where the availability of adequate cell culture models is essential.…”

Differential Growth Properties of Normal and Malignant Esophageal Epithelial Cells: A Possible Cross Talk between Transforming Growth Factor-.BETA.1 and Epidermal Growth Factor Signaling

“…Methods for the primary culture of HEE cells were modifications of previously described procedures (Katayama et al 1984(Katayama et al , 1986Katayama and Kan 1991). The basal medium (HEE basal medium) consisted of hormone-free, Ca ++ -free medium RITC 80-7 (Kan and Yamane 1982) containing CaCl 2 (30 μ M), insulin (1 μ g / ml), transferrin (10 μ g / ml), FeSO 4 7H 2 O (1.5 μ M), L-proline (175 μ g / ml), ethanolamine (10 μ g / ml), DTT (50 μ M), L-ascorbic acid phosphate (100 μ M), and BSA (5 mg / ml).…”

“…Surgically resected esophagi were cut open longitudinally, and then the epithelium was stained with Lugol's solution to distinguish carcinoma from normal epithelium (Brodmerkel 1971). Normal epithelium that was sufficiently apart from the carcinoma was obtained from the esophagus (Katayama et al 1984). The epithelium was minced with knives and digested for 90 min.…”

“…Studies at the cellular and molecular levels are important for advances in the diagnosis and treatment of esophageal carcinomas. To pursue these studies, we previously established a serum-free culture system for normal human esophageal epithelial (HEE) cells (Katayama et al 1984(Katayama et al , 1986, and 15 cell lines (TE-cell lines) from human esophageal carcinomas (Nishihira et al 1993(Nishihira et al , 1994.…”