Novel S100 Proteins in Human Esophageal Epithelial Cells: CAAF 1 Expression is Associated with Cell Growth Arrest.

Hitomi, Jiro; Kimura, Tetsunori; Kusumi, Eiji; Nakagawa, Satoru; Kuwabara, Shirou; Hatakeyama, Katsuyoshi; Yamaguchi, Ken

doi:10.1679/aohc.61.163

Cited by 23 publications

(22 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S100P and S100A12 have been found to be downregulated in ESCC at the protein level and produced by esophageal epithelial cells in the process of cell differentiation. They were considered to be associated with the differentiation of ESCC (Hitomi et al 1998;Sato et al 2002). However, our results only indicated that their expression at the RNA level were downregulated ( p<0.05), and their mean rank score of R C/N took on an increasing tendency from poorly to well via moderately differentiated ESCC.…”

Section: Discussioncontrasting

confidence: 61%

“…Up to now, 21 genes encoding S100 calcium-binding proteins have been identified, and a cluster of 16 S100 genes is mapped to the human chromosome 1q21 region (Gendler et al 1990;Schafer et al 1995;Weterman et al 1996;Pietas et al 2002). Early studies have shown that S100A2, S100A4, S100A12, and S100P proteins were deregulated in esophageal cancer tissues and cell lines and could influence cell differentiation and cell cycle (Kyriazanos et al 2002;Ninomiya et al 2001;Hitomi et al 1998;Sato et al 2002). Moreover, it has been reported that many S100 genes were expressed at high levels in stratified epithelia of the upper digestive tract.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differential expression of S100 gene family in human esophageal squamous cell carcinoma

Zhao²,

Wang

et al. 2004

J Cancer Res Clin Oncol

View full text Add to dashboard Cite

show abstract

Section: Discussioncontrasting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Differential expression of S100 gene family in human esophageal squamous cell carcinoma

Zhao²,

Wang

et al. 2004

J Cancer Res Clin Oncol

View full text Add to dashboard Cite

show abstract

“…This region has revealed a most remarkable density of genes that fulfill important functions in terminal differentiation of the human epidermis [37] . Among those genes, the expression of S100A2, S100A4, S100A12, SPRR3, TGM-3 and other genes has also been identified to be altered in ESCCs [38][39][40][41][42] . Within this chromosomal region, a number of abnormalities such as deletions, rearrangements or translocations were considered to be associated with neoplasia [43,44] .…”

Section: Discussionmentioning

confidence: 99%

Loss of myeloid-related proteins 8 and myeloid-related proteins 14 expression in human esophageal squamous cell carcinoma correlates with poor differentiation

Kong

Ding²,

Zhou³

et al. 2004

WJG

View full text Add to dashboard Cite

AIM:To study the expression of myeloid-related proteins (MRP)8 and myeloid-related proteins(MRP)14 in human esophageal squamous cell carcinoma and to investigate if there was any correlation between MRP8 and MRP14 expression level and histopathological grade in these tumors. METHODS:In this study, 65 cases of advanced esophageal squamous cell carcinoma were assessed for MRP8 and MRP14 expression using immunohistochemistry. Statistical analysis was performed for the comparison of MRP8 and MRP14 expression in normal and tumor tissues, and their relationship with clinicopathological features. RESULTS:Reduced or absent expression of MRP8 and MRP14 was observed in esophageal squamous cell carcinoma, with a significant difference between tumor tissues and normal tissues (P<0.01 and P<0.01 for MRP8 and MRP14, respectively). Poorly differentiated tumors presented a greater decrease than well and moderately differentiated tumors, with a correlation between their protein level and histopathological grading (P<0.001 and P<0.001, respectively). However, no significant association was found between MRP8 and MRP14 expression and age or gender (P>0.05). CONCLUSION:These findings suggest that the decreased expression of MRP8 and MRP14 might play an important role in the pathogenesis of human esophageal squamous cell carcinoma, being particularly associated with poor differentiation of tumor cells.

show abstract

“…Intra-and interassay coefficients of variation for each assay were within the ranges reported by manufacturers. Cal-C ELISAs were performed as previously described (16,17,20).…”

Section: Methodsmentioning

confidence: 99%

Proteomic but Not Enzyme-Linked Immunosorbent Assay Technology Detects Amniotic Fluid Monomeric Calgranulins from Their Complexed Calprotectin Form

Buhimschi

Weiner

et al. 2005

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

Four proteomic biomarkers (human neutrophil peptide 1 [HNP1], HNP2 [defensins], calgranulin C [Cal-C], and Cal-A) characterize the fingerprint of intra-amniotic inflammation (IAI). We compared proteomic technology using surfaced-enhanced laser desorption-ionization-time of flight (SELDI-TOF) mass spectrometry to enzyme-linked immunosorbent assay (ELISA) for detection of these biomarkers. Amniocentesis was performed on 48 women enrolled in two groups: those with intact membranes (n ‫؍‬ 27; gestational age [GA], 26.0 ؎ 0.8 weeks) and those with preterm premature rupture of the membranes (PPROM; n ‫؍‬ 21; GA, 28.4 ؎ 0.9 weeks). Paired abdominal amniotic fluids (aAFs)-vaginal AFs (vAFs) were analyzed in PPROM women. Quantitative aspects of HNP1-3, Cal-C, Cal-A, and calprotectin (a complex of Cal-A with Cal-B) were assessed by ELISA. SELDI-TOF mass spectrometry tracings from 16/48 (33.3%) aAFs and 13/17 (88.2%) vAFs were consistent with IAI (three or four biomarkers present). IAI (by SELDI-TOF mass spectrometry) was associated with increased HNP1-3 and Cal-C measured by ELISA. However, immunoassays detected Cal-A in only 4 of the AFs even though its specific SELDI-TOF mass spectrometry peak was identified in 19/48 AFs. Calprotectin immunoreactivity was decreased in AFs retrieved from women with IAI (P ‫؍‬ 0.01). In conclusion, IAI is associated with increased HNP1-3 levels. In the absence of isoform-specific ELISAs, mass spectrometry remains the only way to discriminate the HNP biomarker isoforms. Monomeric Cal-A is not reliably estimated by specific ELISA as it binds to Cal-B to form the calprotectin complex. Cal-C was reliably measured by SELDI-TOF mass spectrometry or specific ELISA.Premature delivery (PTD; birth before 37 weeks in humans) is a significant public health problem. While complicating 7 to 12% of deliveries, prematurity is associated with 75% of infant mortality and 50% of long-term neurological handicaps, including blindness, deafness, developmental delay, cerebral palsy, and chronic lung disease (4,10,18,22). The etiologies of most preterm births remain unknown (2, 5).Clinically, PTD presents as either preterm labor with intact membranes or with preterm premature rupture of the membranes (PPROM). Several distinct pathophysiological pathways are implicated as triggers for PTD (21): excessive myometrial stretching, decidual hemorrhage, maternal and fetal stress, and inflammation (9). Inflammation may be initiated along various pathways of which infection is but one. Microbial invasion of the amniotic cavity, as evidenced by a positive amniotic fluid (AF) culture, occurs in 10% of the patients with preterm labor and intact membranes and in 38% of the patients with PPROM (25). We previously demonstrated that it is not necessarily the intrauterine infection that causes poor outcomes but rather the resulting intrauterine inflammation which can damage the fetus long before triggering labor (7). Thus, specific and sensitive tests for intrauterine inflammation rather than for bacterial infection may be more ...

show abstract

Novel S100 Proteins in Human Esophageal Epithelial Cells: CAAF 1 Expression is Associated with Cell Growth Arrest.

Cited by 23 publications

References 23 publications

Differential expression of S100 gene family in human esophageal squamous cell carcinoma

Differential expression of S100 gene family in human esophageal squamous cell carcinoma

Loss of myeloid-related proteins 8 and myeloid-related proteins 14 expression in human esophageal squamous cell carcinoma correlates with poor differentiation

Proteomic but Not Enzyme-Linked Immunosorbent Assay Technology Detects Amniotic Fluid Monomeric Calgranulins from Their Complexed Calprotectin Form

Contact Info

Product

Resources

About