Differential expression of S100 gene family in human esophageal squamous cell carcinoma

Ji, Junfang; Zhao, Liqun; Wang, Xiuqin; Zhou, Chen; Ding, Fang; Su, Lei; Zhang, Chunlin; Mao, Xuezheng; Wu, Min; Liu, Zhihua

doi:10.1007/s00432-004-0555-x

Cited by 87 publications

(79 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A better understanding of the mechanisms regulating differentiation would offer the basis for identification of tumor biomarkers. Our previous study showed that S100A14 belongs to a subset of genes that are downregulated in esophageal cancers, and as one of many differentiation-associated genes, reduced S100A14 expression might contribute to esophageal carcinogenesis (17,18). Furthermore, our study showed that S100A14 regulated cell proliferation and apoptosis in a dose-dependent manner via interaction with RAGE in ESCC (15).…”

Section: Discussionmentioning

confidence: 50%

“…Confirmation of the reduced expression of S100A14 in ESCC compared with the matched normal epithelia by qRT-PCR Our previous study showed that S100A14 expression is downregulated in ESCCs versus adjacent normal tissue by semiquantitative RT-PCR (18). To further confirm the differential expression of S100A14 in ESCCs, we performed qRT-PCR analysis in 30 paired ESCCs and adjacent normal epithelial tissues.…”

Section: Resultsmentioning

confidence: 79%

“…Tissue samples from 30 patients with ESCCs were used for S100A14 mRNA expression analysis, and these samples were different from those examined in our previous study (18). Tissue specimens from 110 patients with ESCCs were analyzed by immunohistochemistry (IHC).…”

Section: Tissue Specimensmentioning

confidence: 99%

See 2 more Smart Citations

S100A14: Novel Modulator of Terminal Differentiation in Esophageal Cancer

Chen

Sunkel

et al. 2013

Molecular Cancer Research

Self Cite

View full text Add to dashboard Cite

Aberrant keratinocyte differentiation is a key mechanism in the initiation of cancer. Because activities regulating differentiation exhibit altered or reduced capacity in esophageal cancer cells, it is vital to pinpoint those genes that control epidermal proliferation and terminal differentiation to better understand esophageal carcinogenesis. S100A14 is a member of the S100 calcium-binding protein family and has been suggested to be involved in cell proliferation, apoptosis, and invasion. The present study used immunohistochemistry analysis of S100A14 in clinical specimens of esophageal squamous cell carcinoma (ESCC) to show that decreased S100A14 is strongly correlated with poor differentiation. Furthermore, both mRNA and protein expression of S100A14 was drastically increased upon 12-O-tetra-decanoylphorbol-13-acetate (TPA) and calcium-induced esophageal cancer cell differentiation. Overexpression of S100A14 resulted in a G 1 -phase cell cycle arrest and promoted calcium-inhibited cell growth. Conversely, decreasing S100A14 expression significantly promoted G 1 -S transition and prevented the morphologic changes associated with calcium-induced cell differentiation. Molecular investigation demonstrated that S100A14 altered the calcium-induced expression of late markers of differentiation, with the most prominent effect on involucrin (IVL) and filaggrin (FLG). Finally, it was determined that S100A14 is transcriptionally regulated by JunB and that S100A14 and JunB status significantly correlated in ESCC tissue. In summary, these data demonstrate that S100A14 is transcriptionally regulated by JunB and involved in ESCC cell differentiation.

show abstract

Section: Discussionmentioning

confidence: 50%

Section: Resultsmentioning

confidence: 79%

See 1 more Smart Citation

S100A14: Novel Modulator of Terminal Differentiation in Esophageal Cancer

Chen

Sunkel

et al. 2013

Molecular Cancer Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…We have classified overexpression as expression in a tumor tissue being 1.33-fold higher than that in the normal tissue. (18) Overexpression of S100A10 and B-FABP was confirmed (P < 0.05 and P < 0.005, respectively, χ 2 -test), but annexin II was not defined as overexpressed under this criterion. Then the 47 RCC cases were classified according to the nuclear grade and stage, and overexpression of three genes in each class was summarized (Table 1).…”

Section: Relationship Between Gene Expression and Prognostic Factors mentioning

confidence: 94%

Evaluation of S100A10, annexin II and B‐FABP expression as markers for renal cell carcinoma

et al. 2006

View full text Add to dashboard Cite

This study aimed to analyze expression of S100A10, annexin II and B-FABP genes in renal cell carcinoma (RCC) and their potential value as tumor markers. Furthermore, any correlation between the gene expression and prognostic indicators of RCC was analyzed. Expression of each gene was estimated by RT-PCR in the nonneoplastic (normal) and tumorous parts of resected kidney samples. Also, each antigen was immunostained in RCC and normal kidney tissues. Expression of the S100A10 gene averaged 2.5-fold higher in the tumor than that in the normal tissues (n = 47), after standardization against that of β β β β-actin. However, expression of annexin II, a natural ligand of S100A10, was only 1.64-fold higher. In the tissue sections of RCC, S100A10 and annexin II were immunostained in membranes. In the normal renal epithelia, however, both antigens were stained in the Bowman's capsule and the tubules from Henle's loop through the collecting duct system, but not in the proximal tubules, from where most RCC are derived. In contrast, expression of the B-FABP gene was 20-fold higher in the tumor. No B-FABP was immunohistochemically detected in normal kidney sections, but it was stained in the cytoplasm of RCC tissue sections. S100A10 and B-FABP genes were overexpressed regardless of nuclear grade and stage of RCC. Immunopositivity in RCC tissues (n = 13) was 100% for S100A10 and annexin II, and 70% for B-FABP; however, no clear relationship was observed in either antigen with nuclear grade and stage. It was found that all three performed well as RCC markers. B-FABP was most specific to RCC, as it was expressed little in normal kidney tissues. (Cancer Sci 2007; 98: 77-82) W e have previously described the overexpression of S100A10 in renal cell carcinoma (RCC) cells compared with the normal human kidney cDNA library.(1) Such overexpression has been confirmed in RCC and non-neoplastic (normal) tissues in surgically resected kidneys (n = 7).(1,2) S100A10 is a member of the S100 family of proteins that are small acidic calcium-binding proteins with two helix-loop-helix EF-hand motifs. Twenty human S100 family members are expressed in a cell-and tissue-specific manner, and are responsible for a variety of cellular processes including cell proliferation and differentiation.(3,4) Sixteen of their genes (designated S100A1-S100A16) are clustered on the chromosome 1q21 region, where a number of chromosomal abnormalities occur with neoplasias.(4) S100A10 forms a heterotetramer with annexin II (S100A10) 2 (annexin II) 2 , and the complex localizes in the extracellular membrane of various cancer cells. In the present study, we estimated the gene expression of S100A10 and annexin II by reverse transcription-polymerase chain reaction (RT-PCR) in a larger number of surgically resected RCC samples (n = 47) after standardization for the expression of β-actin in each sample. Then we immunohistochemically investigated the expression of S100A10 and annexin II in RCC and normal kidney tissues (n = 13). The extracellular S100A10/ annexin II complex f...

show abstract

“…More recently, however, S100A2-overexpressing cancers showed a trend toward preferentially developing lymph node metastases and distant metastases, suggesting that S100A2 might be related to the progression of esophageal SCC (Kyriazanos et al, 2002;Ji et al, 2004;Imazawa et al, 2005;Cao et al, 2009). …”

Section: Esophageal Squamous Cell Carcinoma (Escc)mentioning

confidence: 99%