Primary culture media for routine urine processing

Fung, J C; Clark, Edna; Berman, Marjorie; Goldstein, Jerome C.; D'Amato, R F

doi:10.1128/jcm.16.4.632-636.1982

Cited by 11 publications

(4 citation statements)

References 19 publications

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“…In the laboratory, each collected urine specimen was plated onto blood agar medium along with a selective and differential agar such as MacConkey agar for Gram-negative organisms [30]. Urine specimens were plated, using a 0·001 ml calibrated loop.…”

Section: Methodsmentioning

confidence: 99%

Clinical risk factors, bacterial aetiology, and outcome of urinary tract infection in children hospitalized with diarrhoea in Bangladesh

et al. 2016

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Urinary tract infection (UTI) is common in children aged <5 years with diarrhoea, but little is known about risk factors, aetiology and outcome of such children. We aimed to evaluate these knowledge gaps of UTI in children aged <5 years with diarrhoea. We enrolled all children aged <5 years with diarrhoea admitted to Dhaka Hospital of the International Centre for Diarrhoeal Disease Research, Bangladesh, between May 2011 and April 2013, who had history of fever (⩾38 °C) and obtained a urine sample for culture. Diarrhoea with UTI (confirmed by culture) constituted cases (n = 26) and those without UTI constituted controls (n = 78). Threefold controls were randomly selected. The case-fatality rate was comparable in cases and controls (4% vs. 1%, P = 0·439). Escherichia coli (69%) and Klebsiella (15%) were the most commonly isolated pathogens. Persistent diarrhoea, pneumonia and prior antibiotics use were identified as risk factors for UTI in logistic regression analysis (P < 0·05 for all). Thus, children with diarrhoea presenting with persistent diarrhoea, pneumonia, and prior antibiotic use should be investigated for UTI for their prompt management that may reduce morbidity.

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Section: Methodsmentioning

confidence: 99%

Clinical risk factors, bacterial aetiology, and outcome of urinary tract infection in children hospitalized with diarrhoea in Bangladesh

et al. 2016

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“…Samples were plated on Mac-Conkey and Columbia agar plates. 15 Plates were incubated at 35°C and examined for bacterial and fungal growth every 24 hours for up to 5 days. Colony counts were performed on all urine samples from which growth was obtained.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of open versus closed urine collection systems and development of nosocomial bacteriuria in dogs

Sullivan¹,

Campbell²,

Onuma³

2010

javma

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“…Laboratory personnel might consider that urine specimens obtained from female outpatients also be plated on BA. Nevertheless, the much higher isolation rate of etiological agent(s) from urine specimens on CNA than on BA warrants our continued recommendation of the CNA-MAC combination as the primary plating medium for routine urine processing (9).…”

Section: As With Colistin Physiological Concentrations Of Ca2+ Andmentioning

confidence: 98%

Growth of coagulase-negative staphylococci on colistin-nalidixic acid agar and susceptibility to polymyxins

Fung¹,

McKinley²,

Tyburski³

et al. 1984

J Clin Microbiol

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Colistin-nalidixic acid agar, although recently recommended as a replacement for blood agar for primary plating of urine specimens (Fung et al., J. Clin. Microbiol. 16:632-636, 1982), has also been reported to suppress the growth of some strains of staphylococci that are susceptible to colistin (polymyxin E). The susceptibility of 11 species of staphylococci to polymyxins was determined, and the ability of these species to grow on colistin-nalidixic acid agar was examined. Although the MICs for most of the strains tested were 8 ,ug/ml or less, only a few coagulase-negative staphylococci grew on or were inhibited by colistin-nalidixic acid agar. This descrepancy was explained by the antagonistic effects that medium components, such as physiological concentrations of magnesium and calcium and 5% sheep blood, had on the activity of polymyxin. Colistin-nalidixic acid agar is still recommended for routine urine processing; however, the poor growth of 13% of the Staphylococcus saprophyticus strains tested suggests that blood agar should be included in the primary plating battery of urine specimens obtained from female outpatients.

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Primary culture media for routine urine processing

Cited by 11 publications

References 19 publications

Clinical risk factors, bacterial aetiology, and outcome of urinary tract infection in children hospitalized with diarrhoea in Bangladesh

Clinical risk factors, bacterial aetiology, and outcome of urinary tract infection in children hospitalized with diarrhoea in Bangladesh

Evaluation of open versus closed urine collection systems and development of nosocomial bacteriuria in dogs

Growth of coagulase-negative staphylococci on colistin-nalidixic acid agar and susceptibility to polymyxins

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