Growth of coagulase-negative staphylococci on colistin-nalidixic acid agar and susceptibility to polymyxins

Fung, J C; McKinley, G A; Tyburski, M B; Berman, Marjorie; Goldstein, Jerome C.

doi:10.1128/jcm.19.5.714-716.1984

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…In general, Staphylococcus spp. are regarded as poor targets for polymyxins, with high MIC values ranging from 8 to 64 μg/mL . Our results confirm these data, in that the MIC values for the S. pseudintermedius strains from all countries were in the range of 4.3–34.4 μg/mL and for the MRSP strains in the range of 8.6–17.19 μg/mL.…”

Section: Discussionsupporting

confidence: 86%

The joint in vitro action of polymyxin B and miconazole against pathogens associated with canine otitis externa from three European countries

Pietschmann

Meyer

Voget³

et al. 2013

Veterinary Dermatology

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BackgroundCanine otitis externa, an inflammation of the external ear canal, can be maintained and worsened by bacterial or fungal infections. For topical treatment, combinations of anti-inflammatory and antimicrobial ingredients are mainly used.Hypothesis/ObjectivesThis study was conducted to elucidate the in vitro activity of polymyxin B and miconazole against clinical bacterial isolates from three European countries, to investigate possible differences in sensitivity and to assess drug interactions.AnimalsSeventeen strains of Escherichia coli, 24 strains of Pseudomonas aeruginosa, 24 strains of Proteus mirabilis and 25 strains of Staphylococcus pseudintermedius from dogs with diagnosed otitis externa had been isolated in Germany, France and Italy.MethodsDrug activities were evaluated by minimal inhibitory concentration (MIC) and minimal bactericidal concentration. The potentiation of polymyxin B plus miconazole was calculated using the fractional inhibitory concentration index (FICI). An FICI ≤0.5 defined synergy. Furthermore, geographical variations in the FICI and MIC were assessed by statistical analysis.ResultsBacterial susceptibilities were comparable in different European countries, because there were no significant MIC and FICI variations (P > 0.05). As a single agent, polymyxin B had bactericidal activity against most E. coli and P. aeruginosa strains and, in higher concentrations, against S. pseudintermedius strains. Miconazole was bactericidal against all Staphylococcus strains. Synergy was demonstrated against strains of E. coli and P. aeruginosa (FICI = 0.25 and 0.50, respectively), whereas overall there was no interaction against S. pseudintermedius strains (FICI = 1.25). Proteus mirabilis strains were not inhibited by each of the drugs individually or by their combination.Conclusions and clinical importanceIn vitro synergy of polymyxin B and miconazole against E. coli and P. aeruginosa isolates indicates a rationale for applying both agents in combination to treat otitis externa when infected with these types of bacteria.RésuméContexteL'otite externe canine, une inflammation du conduit auriculaire externe, peut être entretenue et aggravée par les infections bactériennes ou fongiques. Pour le traitement topique, les associations d'anti-inflammatoires et d'antimicrobiens sont principalement utilisées.Hypothèses/ObjectifsCette étude a été menée pour déterminer l'activité in vitro de la polymyxine B et du miconazole contre les souches bactériennes cliniques isolées dans trois pays européens, d'étudier les différences possibles de sensibilité et de déterminer les interactions médicamenteuses.SujetsDix-sept souches d'Escherichia coli, 24 souches de Pseudomonas aeruginosa, 24 souches de Proteus mirabilis et 25 souches de Staphylococcus pseudintermedius ont été isolées de chiens atteints d'otite externe en Allemagne, France et Italie.RésultatsL'activité des molécules a été évaluée par la concentration minimale inhibitrice (CMI) et la concentration minimale bactéricide. La potentialisation de la polymyxine...

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Section: Discussionsupporting

confidence: 86%

The joint in vitro action of polymyxin B and miconazole against pathogens associated with canine otitis externa from three European countries

Pietschmann

Meyer

Voget³

et al. 2013

Veterinary Dermatology

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Verarbeitung von klinischen Materialien und Bedeutung der Erregerspektren von Krankheitsbildern für die Diagnostik

Werk¹

1990

Medizinische Bakteriologie Und Infektiologie

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S. aureus colonization in healthy Australian adults receiving an investigational S. aureus 3-antigen vaccine

Marshall

Richmond

Nissen

et al. 2019

Journal of Infection

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Objectives: Assess Staphylococcus aureus (S. aureus) colonization in healthy Australian adults receiving an investigational S. aureus 3-antigen vaccine (SA3Ag). Methods: In this phase 1, double-blind, sponsor-unblinded study, participants were randomized to receive a single dose (1 of 3 dose levels) of SA3Ag or placebo and a booster dose or placebo at 6 months. S. aureus isolates from nasal, perineal, and oropharyngeal swabs before and through 12 months post-vaccination were identified. Results: Baseline S. aureus colonization prevalence was 30.6% (any site), with nasal carriage (27.0%) more common than oropharyngeal/perineal (3.2% each). Following initial vaccination (low-dose: 102; mid-dose: 101; high-dose: 101; placebo: 102) and booster (low-dose: 45; mid-dose: 44; high-dose: 27; placebo: 181), placebo and SA3Ag groups showed similar S. aureus carriage through 12 months. Most colonized participants (74.0%) were colonized by single spa types. Placebo and SA3Ag groups had similar persistence of colonization, with 19.6-30.7% due to single spa types. Acquisition was observed in mid-and highdose recipients (∼20%) and low-dose and placebo recipients (∼12%). Vaccination resulted in substantial increases in antibodies to all 3 antigens, irrespective of carriage status. Conclusions: Based on descriptive analyses of this small study, SA3Ag vaccination did not impact S. aureus acquisition or carriage. Carriage status did not impact antibody responses to SA3Ag.

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Growth of coagulase-negative staphylococci on colistin-nalidixic acid agar and susceptibility to polymyxins

Cited by 3 publications

References 17 publications

The joint in vitro action of polymyxin B and miconazole against pathogens associated with canine otitis externa from three European countries

The joint in vitro action of polymyxin B and miconazole against pathogens associated with canine otitis externa from three European countries

Verarbeitung von klinischen Materialien und Bedeutung der Erregerspektren von Krankheitsbildern für die Diagnostik

S. aureus colonization in healthy Australian adults receiving an investigational S. aureus 3-antigen vaccine

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