Preventive effects of Resveratrol against azoxymethane-induced testis injury in rats

Abstract

To evaluate the protective effects of Resveratrol (RES) on azoxymethane (AOM)-induced testicular damage using histopathology and biochemical analyses, 28 male rats were randomly divided into four groups. Groups were control, RES, AOM and ARES. At the end of the 7 weeks, following routine tissue processing procedure, testis sections were stained with haematoxylin-eosin and Masson's trichrome. The blood samples were taken for biochemical analysis of testosterone, total oxidative stress, total antioxidant status and oxidative stress index. Degenerative changes in the seminiferous tubules such as atrophy, loss in the number of germ cells and arrested spermatogenic cell, and increase in the connective tissue of the tunica albuginea in the groups with AOM treatment were found. RES treatment (ARES) reduced the number of affected seminiferous tubules significantly (p < .05) compared to AOM alone. The testosterone levels in AOM group were significantly lower than in the control group (p < .05). The total oxidative stress levels were significantly higher in AOM group compared to control group (p < .05). The total antioxidant status levels in ARES group were significantly higher than in the AOM group (p < .05). This study results suggest that an antioxidant like Resveratrol may be useful for decreasing the harmful effects of azoxymethane.

Effect of Experimentally Induced Hypothyroidism on Structure of Adult Albino Rats’ Testes and Possible Protective Role of L-carnitine

“…These results are similar to those of Abd Elazeem et al (22) who induced hypothyroidism in 10 rats with carbimazole at a dose of 0.05 mg/kg / day for successive 3 weeks by gastric tube and examined their parotid glands and stated that hypothyroid status caused extensive collagen fibers in between the lobules. This increase in the collagen fibers was explained by Kurus et al (23) who stated that oxidative stress in the testicular tissue promotes excess collagen fibers production. Mallory's trichrome staining of the PTU&LCtreated group showed that LC administration diminished the previous effect in the PTU treated group where the collagen fibers distribution was much less in the capsule.…”

Changes in Adult Rats’ Testis structure Induced by Hypothyroidism and Alleviating Role of L-Carnitine

“…These results are similar to those of Abd Elazeem et al [23] who induced hypothyroidism in 10 rats with carbimazole at a dose of 0.05 mg/kg / day for successive 3 weeks by gastric tube and examined their parotid glands and stated that hypothyroid status caused extensive collagen fibers in between the lobules. This increase in the collagen fibers was explained by Kurus et al [24] who stated that oxidative stress in the testicular tissue promotes excess collagen fibers production.…”