Ameliorate effects of resveratrol and l-carnitine on the testicular tissue and sex hormones level in busulfan induced azoospermia rats

Hafezi, Hananeh; Vahdati, Akbar; Forouzanfar, Mohsen; Shariatic, Mehrdad

doi:10.1016/j.theriogenology.2022.06.006

Cited by 4 publications

(1 citation statement)

References 23 publications

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“…As a result, both oxidative DNA damage and apoptosis‐related, nuclease‐induced DNA fragmentation are observed when the testes is under stress, whether this is chemically, 36–38 psychologically, 39 or surgically 40 induced or the result of exposures to excessive ionizing radiation 41 or heat 42 . Several studies indicate that the germ‐line DNA damage associated with such testicular stress may be alleviated with reagents designed to impede the oxidative damage process such as lactoferrin 36 mitochondrial permeability transition pore inhibitors, 40 curcumin, 43 dimethyl sulfoxide, 41 lutein, 44 vitamin C, 45 vitamin E and vitamin C in combination, 46 silymarin, 47 trigonelline, 42 melatonin, 42 p‐tyrosol, 48 beta‐caryophyllene, 49 saponin 38 and a variety of other antioxidant compounds, administered alone or in combination 50–52 . The ability of commercial antioxidant formulations to resolve the testicular pathology associated with oxidative stress, generated either through the local application of heat or genetic inactivation of glutathione peroxidase 5 (one of the major antioxidant enzymes in the epididymis) has also been clearly demonstrated in animal models 53 …”

Section: Introductionmentioning

confidence: 99%

DNA damage in testicular germ cells and spermatozoa. When and how is it induced? How should we measure it? What does it mean?

Aitken

Lewis

2023

Andrology

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This review surveys the causes and consequences of DNA damage in the male germ line from spermatogonial stem cells to fully differentiated spermatozoa. Within the stem cell population, DNA integrity is well maintained as a result of excellent DNA surveillance and repair; however, a progressive increase in background mutation rates does occur with paternal age possibly as a result of aberrant DNA repair as well as replication error. Once a germ cell has committed to spermatogenesis, it responds to genetic damage via a range of DNA repair pathways or, if this process fails, by the induction of apoptosis. When fully‐differentiated spermatozoa are stressed, they also activate a truncated intrinsic apoptotic pathway which results in the activation of nucleases in the mitochondria and cytoplasm; however, the physical architecture of these cells prevents these enzymes from translocating to the nucleus to induce DNA fragmentation. Conversely, hydrogen peroxide released from the sperm midpiece during apoptosis is able to penetrate the nucleus and induce DNA damage. The base excision repair pathway responds to such damage by cleaving oxidized bases from the DNA, leaving abasic sites that are alkali‐labile and readily detected with the comet assay. As levels of oxidative stress increase and these cells enter the perimortem, topoisomerase integrated into the sperm chromatin becomes activated by SUMOylation. Such activation may initially facilitate DNA repair by reannealing double strand breaks but ultimately prepares the DNA for destruction by nucleases released from the male reproductive tract. The abasic sites and oxidized base lesions found in live spermatozoa are mutagenic and may increase the mutational load carried by the offspring, particularly in the context of assisted conception. A variety of strategies are described for managing patients expressing high levels of DNA damage in their spermatozoa, to reduce the risks such lesions might pose to offspring health.

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Section: Introductionmentioning

confidence: 99%

DNA damage in testicular germ cells and spermatozoa. When and how is it induced? How should we measure it? What does it mean?

Aitken

Lewis

2023

Andrology

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Ameliorating effects of selenium nanoparticle coated by gallic acid on histological and biochemical parameters of testis in azoospermic rat model

Vatanpour,

Ebrahimzadeh-bideskan,

Rajabian

et al. 2024

Tissue and Cell

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Resveratrol ameliorates atrazine-induced caspase-dependent apoptosis and fibrosis in the testis of adult albino rats

Hassanin,

Kamal,

Ismail

2024

Sci Rep

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Pesticides like atrazine which are frequently present in everyday surroundings, have adverse impacts on human health and may contribute to male infertility. The work aimed to analyze the histological and biochemical effects of atrazine on the testis in adult albino rats and whether co-administration with resveratrol could reverse the effect of atrazine. Forty adult male albino rats in good health participated in this study. They were categorized at random into four groups: the Group Ӏ received water through a gastric tube for two months every day, the Group ӀӀ received resveratrol (20 mg/kg body weight (b.w.)) through a gastric tube for two months every day, the Group ӀӀӀ received atrazine (50 mg/kg bw) through a gastric tube for two months every day, the Group ӀV received concomitant doses of atrazine and resveratrol for two months every day. The testes of the animals were then carefully removed and prepared for biochemical, immunohistochemical, light, and electron microscopic studies. Atrazine exposure led to a significant decrease in serum testosterone hormone level, upregulation of caspase 3 and iNOS mRNA levels, destructed seminiferous tubules with few sperms in their lumens, many collagen fibres accumulation in the tunica albuginea and the interstitium, abnormal morphology of some sperms as well as many vacuolations, and damaged mitochondria in the cytoplasm of many germ cells. Concomitant administration of resveratrol can improve these adverse effects. It was concluded that atrazine exposure is toxic to the testis and impairs male fertility in adult rat and coadministration of resveratrol guards against this toxicity.

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Ameliorate effects of resveratrol and l-carnitine on the testicular tissue and sex hormones level in busulfan induced azoospermia rats

Cited by 4 publications

References 23 publications

DNA damage in testicular germ cells and spermatozoa. When and how is it induced? How should we measure it? What does it mean?

DNA damage in testicular germ cells and spermatozoa. When and how is it induced? How should we measure it? What does it mean?

Ameliorating effects of selenium nanoparticle coated by gallic acid on histological and biochemical parameters of testis in azoospermic rat model

Resveratrol ameliorates atrazine-induced caspase-dependent apoptosis and fibrosis in the testis of adult albino rats

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