Preventive effect of S-allyl cysteine sulfoxide (alliin) on cardiac marker enzymes and lipids in isoproterenol-induced myocardial injury

Abstract

The present study was designed to evaluate the preventive effect of S-allyl cysteine sulfoxide (SACS) in isoproterenol (ISO)-induced myocardial ischaemia in male Wistar rats. Rats were pretreated with SACS (40 and 80 mg kg(-1) body-weight) for 5 weeks. After the treatment period, ISO (150 mg kg(-1) body-weight) was administered subcutaneously to rats at intervals of 24 h for 2 days. The activities of creatine kinase, creatine kinase-MB, lactate dehydrogenase, aspartate transaminase and alanine transferase were significantly increased in serum and significantly decreased in the hearts of ISO-treated rats. Pretreatment with SACS decreased the activities of these enzymes significantly in serum and significantly increased the activities in heart in ISO-treated rats. The levels of cholesterol, triglycerides and free fatty acids increased in serum and heart, while the levels of phospholipids increased in serum and decreased in heart in ISO-treated rats. SACS pretreatment showed a significant effect on the lipids studied. The activity of 3-hydroxy 3-methyl glutaryl coenzyme A (HMG CoA) reductase was significantly increased and the activity of lecithin cholesterol acyl transferase (LCAT) was significantly reduced in ISO-induced rats. Oral pretreatment with SACS significantly decreased the activity of HMG CoA reductase and significantly increased the activity of LCAT in ISO-induced rats. The levels of plasma thiobarbituric acid reactive substances and hydroperoxides were increased in ISO-treated rats. Pretreatment with SACS significantly decreased the levels of lipidperoxides in ISO-treated rats. The effect at a dose of 80 mg kg(-1) body-weight was more effective than at a dose of 40 mg kg(-1) body-weight and brought back all the biochemical parameters to near normal levels. Thus our study shows that SACS has a lipid-lowering effect in ISO-induced rats. Our study may have clinical relevance.

Antilipoperoxidative and antioxidant effects of S-allyl cysteine sulfoxide on isoproterenol-induced myocardial infarction in wistar rats

“…Rats were administered ISO at a dose of 150 mg kg −1 body weight dissolved in 2 mL of normal saline subcutaneously twice at an interval of 24 h [10].…”

“…Group I: normal untreated rats given orally 2 mL of saline by gastric intubation daily for a period of 35 days; groups II and III: normal rats treated with SACS (40 mg kg −1 and 80 mg kg −1 , respectively) in 2 mL of saline by gastric intubation daily for a period of 35 days [10]; group IV: rats were subcutaneously injected with isoproterenol in 2 mL of saline (150 mg kg −1 ) once a day for 2 days; groups V and VI: rats pretreated with SACS (40 mg kg −1 and 80 mg kg −1 , respectively) in 2 mL of saline by gastric intubation daily for a period of 35 days and then subcutaneously injected with ISO (150 mg kg −1 ) once a day for two days [10].…”

“…In our lab, we have already reported that SACS exhibits protective effect on cardiac marker enzymes, lipids, lipoproteins, and enzymes associated with lipid metabolism (3-hydroxy-3-methyl glutaryl coenzyme A reductase and lecithin cholesterol acyl transferase) in ISO-induced myocardial infarcted male Wistar rats [10]. Extensive literature surveys have shown that there are no available scientific reports on the effect of SACS on ISOinduced MI.…”

Protective effect of S‐allyl cysteine sulphoxide (alliin) on glycoproteins and hematology in isoproterenol induced myocardial infarction in male Wistar rats

“…Jennings et al (1986) have shown that inhibition of the sodium pump may precipitate an excess of internal sodium. In this context, it was reported earlier that the levels of FFAs increased in ISO-treated rats (Sangeetha and Darlin Quine, 2006a).…”

Effect of Alliin on Vascular Functions

“…또한 마늘 추출물은 isoproterenol 유발 심근이상 억제, 고지혈증 감소효과를 일으키는 등, 죽상동맥경화와 관련한 여 러 심혈관 질환을 예방하고 치료하는데 효과가 있다고도 알려 져 있다 [2,6,19]. 마늘추출물을 이용한 세포실험결과에서도 콜 레스테롤합성의 중요 조절 효소인 hydroxymethyl-glutarylCoA reductase 억제효과가 관찰되는 등 [8], 마늘의 주요 성분 이 심혈관 기능과 밀접하게 연계되어 있음을 알 수 있다.…”