Prevention of melamine-induced hepatorenal impairment by an ethanolic extract of Moringa oleifera: Changes in KIM-1, TIMP-1, oxidative stress, apoptosis, and inflammation-related genes

Abd‐Elhakim, Yasmina M.; Mohamed, Wafaa A.M.; Bohi, Khlood M. El; Ali, Haytham; Mahmoud, Fagr A.; Saber, Taghred M.

doi:10.1016/j.gene.2020.145083

Cited by 28 publications

(19 citation statements)

References 107 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, our results suggest that melamine elevates the susceptibility of tissue to oxidative damage via increasing oxidative stress and reduction of endogenous antioxidant capacity in both tissues. Our findings coincide with the previous results stated by Al-Seeni et al and Abd-Elhakim et al [21,64].…”

Section: Discussionsupporting

confidence: 94%

“…Our results reveal that melamine-intoxicated rats exhibited a significant elevation in the serum creatinine and urea levels, indicating melamine-induced renal glomerular impairment. These findings are consistent with those of Al-Seeni et al and Abd-Elhakim et al [21,64]. Moreover, the renal damage induced by melamine is indicated histopathologically by severe deterioration of renal architecture as shown in Fig.…”

Section: Discussionsupporting

confidence: 92%

“…9 (d-j) that indicated severe damage of the cellular membrane followed by leakage of hepatic enzymes into the bloodstream [57]. These results are in good agreement with those reported recently by Abd-Elhakim et al [21]. However, treatment with Se-NPs@starch in group IV markedly decreased the serum activities of both ALT and AST, in addition to improving liver functions, which are highly indicative of the hepatoprotective effect of Se-NPs@starch on melamine-induced hepatic impairment.…”

Section: Discussionsupporting

confidence: 91%

“…Moreover, the renal damage induced by melamine is indicated histopathologically by severe deterioration of renal architecture as shown in Fig. 10 (c-g & k) which was also observed by Lee et al [65], Abd-Elhakim et al [21], Lee et al. [65], Yasui et al [66] and Peerakietkhajorn et al [67].…”

Section: Discussionsupporting

confidence: 68%

See 3 more Smart Citations

Protective Effect of Starch-stabilized Selenium Nanoparticles against Melamine-induced Hepato-renal Toxicity in Male Albino Rats

Ahmed

Galal

Drweesh

et al. 2021

Preprint

View full text Add to dashboard Cite

Melamine and its analogues are illegally added to raise the apparent protein content in foods. The elevated concentrations of these compounds cause adverse effects in humans and animals. In this contribution, the protective effects of the synthesized starch-stabilized selenium nanoparticles (Se-NPs@starch) on melamine-induced hepato-renal toxicity have been systematically investigated. The Se-NPs@starch were characterized by X-ray photoelectron spectroscopy (XPS) analysis, energy dispersive spectroscopy (EDS) mapping analysis, TEM, and FT-IR. Starch plays a crucial role in the stabilization and dispersion of Se NPs, as noticed from the TEM and EDS investigations. Furthermore, the atomic ratio of Se distribution over the starch surface is approximately 1.67%. The current study was conducted on four groups of adult male rats, and the oral daily treatments for 28 days were as follows: group I served as control, group II received Se-NPs@starch, group III was exposed to melamine, while group IV was treated with melamine and Se-NPs@starch. The results reveal a significant alteration in the histoarchitecture of both hepatic and renal tissues induced by melamine. Furthermore, elevated liver and kidney function markers, high malondialdehyde, and increased expression levels of apoptosis-related genes besides a reduction in GSH and expression levels of antioxidant genes were observed in the melamine-exposed group. Interestingly, the administration of the Se-NPs@starch resulted in remarkable protection of rats against melamine-induced toxicity through increasing the antioxidant capacity and inhibiting oxidative damage. Collectively, this study provides affordable starch-stabilized Se-NPs with potent biological activity, making them auspicious candidates for prospective biomedical applications.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 68%

See 2 more Smart Citations

Protective Effect of Starch-stabilized Selenium Nanoparticles against Melamine-induced Hepato-renal Toxicity in Male Albino Rats

Ahmed

Galal

Drweesh

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…In recent years, several natural products showed strong nephroprotective activity [ 10 , 11 , 12 , 13 ]. Curcumin (CUR), the Curcuma longa Linn yellow pigment, has attracted much attention for medicinal purposes in a wide range of illnesses such as autoimmune diseases [ 14 ], cancer [ 15 ], diabetes mellitus [ 16 ], and fatty liver disease [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Curcumin against Sodium Salicylate-Induced Oxidative Kidney Damage, Nuclear Factor-Kappa Dysregulation, and Apoptotic Consequences in Rats

et al. 2021

Self Cite

View full text Add to dashboard Cite

This study examined the effect of sodium salicylates (SS), alone and in combination with curcumin (CUR), on kidney function and architecture in rats. Five rat groups were given 1 mL physiological saline/rat orally, 1 mL olive oil/rat orally, 50 mg CUR/kg bwt orally, 300 mg SS/kg bwt intraperitoneally, or CUR+SS for 15 days. The hematological indices, serum protein profile, serum electrolytes balance, oxidative stress, and lipid peroxidation of kidney tissues were assessed. The histopathological examination and immune expression of Caspase-3 and nuclear factor kappa (NF-κB) were conducted. The findings showed that SS injection induced nephrotoxic activity, including increased serum urea, creatinine, and uric acid levels. It also caused apparent pathological alterations with increased Caspase-3 and NF-κB immuno-expression. In addition, thrombocytopenia, leukocytosis, neutrophilia, hyponatremia, hypochloremia, hypocalcemia, and hypomagnesemia but not hyperkalemia and hyperphosphatemia were evident in SS-injected rats. Moreover, SS exposure increased serum α1 globulin, renal tissue malondialdehyde, and Caspase-3 levels but superoxide dismutase, glutathione peroxidase, and Bcl-2 levels declined. Meanwhile, CUR significantly counteracted the SS harmful impacts on kidneys but SS+CUR co-administration induced an anemic condition. Overall, CUR has an evident protective role against SS-induced renal damage, but the disturbed hematological alterations should be carefully taken into consideration in their combined use.

show abstract

Assessment of hepatoprotective, nephroprotective efficacy, and antioxidative potential of Moringa oleifera leaf powder and ethanolic extract against PCOS‐induced female albino mice (Mus Musculus)

Khalid,

Arshad,

Raza

et al. 2023

Food Science & Nutrition

View full text Add to dashboard Cite

Moringa oleifera is a medicinal plant that has anti‐inflammatory, antihypertensive, antidiabetic, tissue‐protective, and antioxidant activities. Here, we evaluated the protective effect of M. oleifera leaf powder (MoLP) and 70% ethanol M. oleifera leaf extract (MoLE) on mitigating polycystic ovary syndrome (PCOS)‐induced liver and kidney dysfunction via regulating oxidative stress in female albino mice (Mus musculus). The efficacy of M. oleifera was compared with metformin (standard medicine used to treat infertility in women). PCOS was induced by intramuscular injection of testosterone enanthate at 1.0 mg/100 g BW for 35 days. PCOS‐induced mice were treated with MoLP (250 and 500 mg/Kg), MoLE (250 and 500 mg/kg), and metformin (250 mg/kg) orally for 14 days. Renal function test (RFT), liver function test (LFT), and oxidative stress biomarker malondialdehyde (MDA) were quantified in serum at 0, 7, and 14 days of intervention. Mice treated with M. oleifera and metformin showed a significant decrease (p < .001) in alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphate (ALP), total bilirubin, urea, creatinine, and a significant increase (p < .001) in total protein, albumin, globulin, and albumin/globulin (A/G) ratio. Oxidative stress decreased significantly (p = .00) with respect to treatments, exposure days, and their interaction in metformin and all M. oleifera‐treated groups. M. oleifera leaf powder and extract reduce oxidative stress and enhance nephron‐hepatic activity in PCOS‐induced female albino mice.

show abstract

Prevention of melamine-induced hepatorenal impairment by an ethanolic extract of Moringa oleifera: Changes in KIM-1, TIMP-1, oxidative stress, apoptosis, and inflammation-related genes

Cited by 28 publications

References 107 publications

Protective Effect of Starch-stabilized Selenium Nanoparticles against Melamine-induced Hepato-renal Toxicity in Male Albino Rats

Protective Effect of Starch-stabilized Selenium Nanoparticles against Melamine-induced Hepato-renal Toxicity in Male Albino Rats

Protective Effect of Curcumin against Sodium Salicylate-Induced Oxidative Kidney Damage, Nuclear Factor-Kappa Dysregulation, and Apoptotic Consequences in Rats

Assessment of hepatoprotective, nephroprotective efficacy, and antioxidative potential of Moringa oleifera leaf powder and ethanolic extract against PCOS‐induced female albino mice (Mus Musculus)

Contact Info

Product

Resources

About