Prevention of Intraperitoneal Adhesions and Abscesses by Polysaccharides Isolated From Phellinus spp in a Rat Peritonitis Model

Bae, Ji‐Hoon; Ahn, So-Jeo; Yim, Hyunee; Jang, Kwang-Ho; Jin, Hee Kyung

doi:10.1097/01.sla.0000154281.79639.89

Cited by 34 publications

(32 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among these Phellinus mushrooms, PG used in this study has advantages over the other Phellinus mushrooms in that it has a very short growth period (3 months) compared to other Phellinus mushrooms (2-3 years) making it cheaper to produce. In addition, our recent studies have demonstrated significant anti-inflammatory properties of PG such as inhibition of pulmonary inflammation [11] and prevention of intraperitoneal adhesion under infection [1,3,6]. We therefore predict that medicinal application of PG has many advantages in medical cost-cutting as well as benefits in clinical application in future.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Promotion of Dermal Wound Healing by Polysaccharides Isolated from Phellinus gilvus in rats

Bae

Jang

Park

et al. 2005

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. The effects of topical administration of polysaccharides isolated from fungus, Phellinus gilvus (PG) on the healing of rat dermal wounds were assessed. In 10 Sprague-Dawley (SD) rats, six 6 mm diameter defects were made with a punch biopsy appliance. After 24 hr, test substances were applied to the defects twice a day: 0.025, 0.25, and 2.5% polysaccharides from PG (PG0.025, 0.25, and 2.5 groups), Madecassol ® ointment (MC group), aqueous gel (AG group) and no treatment (control group). Six days postoperatively, the contraction and reepithelialization of the wound surface were assessed. Wound diameter was significantly reduced in all PG groups (P<0.05). Complete epithelialization and macrophages were noted in the PG0.25 group, as compared to the control group. We conclude that polysaccharides isolated from PG have significant dermal wound healing effects, and this investigation suggests the potential clinical application of PG as a wound healing agent.

show abstract

mentioning

confidence: 99%

“…We have previously demonstrated that polysaccharides isolated from PG inhibit pulmonary inflammation [11] and intraperitoneal adhesion related to intraperitoneal inflammation [1,3,6]. These effects of PG related to anti-inflammatory activities might be beneficial in the treatment of dermal wound healing.…”

mentioning

confidence: 99%

Promotion of Dermal Wound Healing by Polysaccharides Isolated from Phellinus gilvus in rats

Bae

Jang

Park

et al. 2005

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Phellinus gilvus (PG) is a medicinal mushroom belonging to Hymenochaetaceae basidiomycetes , and has advantages over many Phellinus species due to its short growth period (3 mo), making it cheaper to produce (1)(2)(3). Previous studies in our laboratory and by other investigators have established various biological activities of PG.…”

mentioning

confidence: 99%

In Vitro Antioxidant and Anti-Inflammatory Activities of Protocatechualdehyde Isolated from Phellinus gilvus

Chang

Gebru

Lee

et al. 2011

J Nutr Sci Vitaminol

View full text Add to dashboard Cite

Summary Although various biological activities of Phellinus gilvus (PG) have been reported, the active compounds responsible for these effects are not known. Here, we evaluated the activity of various solvent extracts of PG, and found the ethyl acetate extract (Fd) to be the most active fraction, showing a strong DPPH free radical scavenging activity, and inhibitory effects on LPS-induced nitric oxide (NO) production and COX-2 mRNA expression in RAW264.7 macrophages. Six major compounds were identified from the ethyl acetate extract of PG, and protocatechualdehyde (PCA) was supposed to be the major phenolic compound of PG responsible for its DPPH free radical scavenging activity and its inhibitory effects on LPS-induced NO production in RAW264.7 cells. Further in vitro and in vivo experiments are currently underway to confirm this observation and to investigate the detailed molecular mechanisms involved in the process as well as the biological activities of other fractions of Fd. Key Words Phellinus gilvus , protocatechualdehyde, DPPH, NO, RAW264.7 cellsPhellinus gilvus (PG) is a medicinal mushroom belonging to Hymenochaetaceae basidiomycetes , and has advantages over many Phellinus species due to its short growth period (3 mo), making it cheaper to produce ( 1-3 ). Previous studies in our laboratory and by other investigators have established various biological activities of PG. Among the reported benefits of PG are inhibition of pulmonary inflammation, prevention of intraperitoneal adhesion under infectious circumstances, promotion of dermal wound healing, anti-platelet aggregation and antitumor activities ( 1-4 ). In a previous study with various solvent extracts of PG, we have demonstrated marked DPPH free radical scavenging and xanthine oxidase (XO)-inhibitory activities of the ethyl acetate extract of PG, along with the enhancement of cellular immunity by hot water extracts of PG and inhibition of thrombin-induced platelet aggregation by chloroform-, methanol-and butanol extracts of PG ( 3 ). In the current investigation, we determined the major components of the ethyl acetate extract of PG responsible for its biological activities and further compared the magnitude of the antioxidant/anti-inflammatory activities of components with the various fractional extracts of PG.The fruiting body of PG was provided by Gyeongbuk Agriculture Technology Administration (Daegu, Korea). The preparation of PG fractional extracts was reported earlier ( 3 ). Briefly, fruiting bodies of the mushroom were cut into small pieces and dried at 40-50˚C for 48 h. The pieces were homogenized, extracted with water (1 : 25) at 100˚C for 10 h, and the aqueous phase (Fa) concentrated at 80˚C in a rotary evaporator (Büchi Rotavapor R-114, Switzerland) and the final volume reduced to 1/10 of the initial volume. The Fa was then mixed with 95% ethanol (1 : 3, v/v) and stored at 4˚C overnight. The solution was then centrifuged at 15,000 ϫ g for 30 min, the precipitate dialyzed (1 : 100,000) in water, filtered, centrifuged, re-dissolved and ly...

show abstract

“…23 Although several manouvers have been alleged to lessen the adhesion formation including the use of anti-inflammatory agents, antioxidants, anticoagulants, fibrinolytics, and mechanical barriers, the golden standard has not been confirmed yet. [24][25][26] MB has been utilized in numerous clinical fields including the management of both cyanide and nitrite poisoning as well as urinary tract infections. 27 It antagonizes the effects of nitric oxide and it also competitively inhibits the reduction of molecular oxygen to superoxide by acting as an electron acceptor for xantine oxidase.…”

Section: Discussionmentioning

confidence: 99%

Effect of methylene blue on intra-abdominal adhesion formation in rats

Mahdy

Mohamed

Elhawary

2008

International Journal of Surgery

View full text Add to dashboard Cite

a b s t r a c tBackground: Adhesion formation represents an important clinical challenge in gastro-intestinal surgery. In the late era, methylene blue (MB) has been claimed to play a helping role in combating such a problem. In the present study, we investigated the effects of MB administered intraperitoneally on the surgically-induced adhesions in rats and tried to clarify whether its impact is concentration-dependent.Material & Methods: Seventy adult male Wistar rats were used. They were allocated into seven groups, ten rats each: one control, two sham-operated and four experimental ones. Peritoneal adhesions were evoked by scraping the cecal serosa in the four experimental groups which were given different concentrations of MB, intraperitoneally. The animals of the control group were similarly dealt but were given saline. The rats of the sham-operated groups were given either MB or saline but without any serosal damage.Results: After the elapse of two weeks, the resulting adhesions, inflammation and fibrosis were evaluated. In the rats treated with MB 1%, adhesions, inflammatory process and fibrosis scores were similar to those in the sham-operated rats and were significantly lower than those in all other groups.Conclusion: It could be concluded that MB, 1%, had the best anti-adhesion potential.

show abstract

Prevention of Intraperitoneal Adhesions and Abscesses by Polysaccharides Isolated From Phellinus spp in a Rat Peritonitis Model

Abstract: We concluded that PG and PL had significant adhesion- and abscess-reducing effects and may act by modulating fibrinolytic capacity of uPA and/or tPA produced from macrophages in a rat peritonitis model.

Cited by 34 publications

References 23 publications

Promotion of Dermal Wound Healing by Polysaccharides Isolated from Phellinus gilvus in rats

Promotion of Dermal Wound Healing by Polysaccharides Isolated from Phellinus gilvus in rats

In Vitro Antioxidant and Anti-Inflammatory Activities of Protocatechualdehyde Isolated from Phellinus gilvus

Effect of methylene blue on intra-abdominal adhesion formation in rats

Contact Info

Product

Resources

About