Prevalência de bacilos Gram-negativos não fermentadores de pacientes internados em Porto Alegre-RS

Deliberali, Bruno; Myiamoto, Kendi Nishino; Neto, Carlos Hugo Del Priore Winckler; Pulcinelli, Rafael Silvio Remus; Aquino, Alzira Resende do Carmo; Vizzotto, Bruno Stefanello; Santos, Roberto Christ Vianna

doi:10.1590/s1676-24442011000500006

Cited by 9 publications

(9 citation statements)

References 25 publications

(33 reference statements)

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“…These samples are usually collected from mechanically ventilated patients, and in such cases, the airways are often colonized by NFGNB, especially P. aeruginosa 13 . Similar observations were reported by Deliberali et al, where most NFGNB strains were isolated from tracheal aspirate (38.3%) 11 . The Figures compare the proportions of antimicrobialresistant MBL-producing Acinetobacter (Figure 1) and P. aeruginosa (Figure 2) strains isolated in 2008 and 2013.…”

supporting

confidence: 90%

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Prevalence and antimicrobial susceptibility of non-fermenting Gram-negative bacilli isolated from clinical samples at a tertiary care hospital

Argenta

Fuentefria

Sobottka

2017

Rev. Soc. Bras. Med. Trop.

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Introduction:We compared the prevalence and antimicrobial susceptibility of non-fermenting gram-negative bacilli (NFGNB) isolated from clinical samples at a Brazilian tertiary care hospital in 2008 and 2013. Methods: Collected data included patient's name, age, sex, inpatient unit, laboratory record number, type of biological material, culture test result, and antimicrobial susceptibility of isolated strains. Results: Out of 19,112 culture tests analyzed, 926 (4.8%) were positive for NFGNB. Among these, 45.2% were metallo-beta-lactamase (MBL) producing strains. Conclusion: Between 2008 and 2013, the number of MBL-producing NFGNB isolates increased by 21.5%, which was accompanied by a consequent reduction in susceptibility to antimicrobials.

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supporting

confidence: 90%

“…(22.4%; 50/223). Deliberali et al found that NFGNB comprised 2.2% of all strains isolated between 2006 and 2008 from a hospital in Porto Alegre, Rio Grande do Sul, Brazil 11 . P. aeruginosa was also the predominant species (65%), followed by A. baumannii (16.5%).…”

Section: Outmentioning

confidence: 99%

Prevalence and antimicrobial susceptibility of non-fermenting Gram-negative bacilli isolated from clinical samples at a tertiary care hospital

Argenta

Fuentefria

Sobottka

2017

Rev. Soc. Bras. Med. Trop.

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“…Also, 34.7% (25/72) of the analyzed samples were from tracheal secretion, 23.6% (17/72) from rectal swab, 15.2% (11/72) from urine, 13.9% (10/72) from blood, 8.3% (6/72) from catheter tips, and 4.16% (3/72) from other body fluids. Similarly, studies demonstrate that samples from tracheal secretion, rectal swab, and urine of hospitalized patients present high frequencies of NFGNB related to colonization or infection ( Figure 1 ) [ 2 , 6 , 25 ].…”

Section: Resultsmentioning

confidence: 98%

“…Over the last 20 years, the numbers of multidrug-resistant bacteria from these species have gradually increased around the world, especially in developing countries [ 25 ]. Deliberali et al [ 25 ] identified NFGNB in 326 samples from patients admitted in a hospital in Porto Alegre-MG, Brazil. Among these samples, P. aeruginosa was identified in 65% of the isolates and A. baumannii in 16.6%.…”

Section: Resultsmentioning

confidence: 99%

“…The increasing number of studies which report the alarming amounts of polymyxin B-resistant Gram-negative bacteria from clinical isolates stimulates the scientific community to develop new low-cost and rapid methods that can be used for clinical diagnosis [ 25 , 26 ]. In the present study, 26.4% of 34 P. aeruginosa isolates (9/34) were positive for the polymyxin B agar-spot test presenting more than one bacterial colony, whereas among 38 isolates of Acinetobacter spp., 13.2% (5/38) were positive.…”

Section: Resultsmentioning

confidence: 99%

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Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa

Júnior

Silva

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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The present study aimed to characterize the susceptibility profile of Pseudomonas aeruginosa and Acinetobacter spp. clinical isolates to polymyxin B in a public hospital in Recife-PE, Brazil, between the years of 2018 and 2019, as well as to search for the presence of the mcr-1 gene and evaluate the interaction between polymyxin B and usnic acid against these isolates. The strains were identified using the BD Phoenix™ automated system and the agar-spot test was used to determine the susceptibility profile to polymyxin B. The minimum inhibitory concentrations (MICs) of usnic acid and polymyxin B were determined through the broth microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). Subsequently, Polymerase Chain Reaction (PCR) was performed to detect the mcr-1 gene in the isolates. The interaction between usnic acid and polymyxin B was evaluated by the Checkerboard assay. Among 34 isolates of P. aeruginosa, 26.5% (9/34) were positive for the polymyxin B agar-spot test, and 11.8% (4/34) presented an intermediate susceptibility (MIC = 4 μg/mL), while 14.7% (5/34) presented antimicrobial resistance with MIC values ranging from 8 to 32 μg/mL. Among 38 isolates of Acinetobacter spp., 13.2% (5/38) were positive for the polymyxin B agar-spot test and all of them were resistant to polymyxin B with a MIC value > 32 μg/mL. The mcr-1 gene was not detected in the clinical isolates. Regarding usnic acid, it presented a moderate antibacterial activity against two P. aeruginosa isolates (MIC = 250 μg/mL) and no activity was detected against the others. A synergistic effect between usnic acid and polymyxin B was observed against three clinical isolates of P. aeruginosa which were resistant to polymyxin B (FICI ≤ 0.5). Therefore, it was possible to observe that usnic acid is a promising candidate to be used in combination with polymyxin B against infections caused by resistant P. aeruginosa.

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Immediate and later impacts of antimicrobial consumption on carbapenem-resistant Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella spp. in a teaching hospital in Brazil: a 10-year trend study

Federico

Furtado

2018

Eur J Clin Microbiol Infect Dis

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To evaluate trends and the immediate and late impact of antimicrobial consumption on carbapenem-resistant Acinetobacter spp. (CRAs), carbapenem-resistant Pseudomonas aeruginosa (CRPA), and carbapenem-resistant Klebsiella spp. (CRKs) over a 10-year period. An ecological study was conducted at the teaching hospital in São Paulo, Brazil, from 2007 to 2016. Consumption and resistance data were collected from the supply sector and central laboratory of the institution, respectively. Associations between consumption and resistance were analyzed in the same year, 1 year later, and 2 years later by linear regression of mixed effects. A total of 22,041 isolates were analyzed. Among these, 9988 corresponded to the gram-negatives in this study [3682 (36.9%) were Klebsiella spp., 3169 (31.7%) were P. aeruginosa, and 3137 (31.4%) were Acinetobacter spp.]. An increasing trend of consumption was observed, except for fourth-generation cephalosporins. Carbapenems were the most used antimicrobial class; CRKs presented a substantial increase over this period (from 1.4 to 67.0%; p = 0.001). Increased consumption of third-generation cephalosporins reduced CRAs [- 2.43%, 95% confidence interval (CI), - 3.30 to - 1.57; p < 0.001] and increased CRPA [26.67%, 95% CI, 2.99 to 50.35; p = 0.034] in the same year. Increased consumption of β-lactam/β-lactamase inhibitors increased CRKs with a 1-year delay [5.13%, 95% CI, 2.40 to 7.86; p = 0.001]. Our study demonstrated high antimicrobial consumption and growing carbapenem-resistance rates among gram-negative bacteria, especially Klebsiella spp., and the immediate and later effects of consumption of multiple antimicrobials on carbapenem resistance.

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Prevalência de bacilos Gram-negativos não fermentadores de pacientes internados em Porto Alegre-RS

Cited by 9 publications

References 25 publications

Prevalence and antimicrobial susceptibility of non-fermenting Gram-negative bacilli isolated from clinical samples at a tertiary care hospital

Prevalence and antimicrobial susceptibility of non-fermenting Gram-negative bacilli isolated from clinical samples at a tertiary care hospital

Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa

Immediate and later impacts of antimicrobial consumption on carbapenem-resistant Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella spp. in a teaching hospital in Brazil: a 10-year trend study

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