Prevalence of β-lactam and fluoroquinolone resistance, and virulence factors in Escherichia coli isolated from chickens in Slovakia

Drugdova, Zuzana; Kmet, V.

doi:10.2478/s11756-012-0142-6

Cited by 14 publications

(9 citation statements)

References 50 publications

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“…Sulfonamide resistance is conferred by sul1 and sul2 ( Shin et al., 2014 ). In this study, the sul2 gene was detected in a larger proportion of the isolates, and the more frequent presence of sul2 than sul1 has also been reported in previous studies about E. coli from poultry industry ( Guerra et al., 2003 , Drugdová and Kmeť, 2013 ). The sul1 gene, however, is often found together with other antimicrobial resistance genes in gene cassettes in the carriable components of class 1 integrons ( Poirel et al., 2008 ).…”

Section: Discussionsupporting

confidence: 87%

Molecular characterization of avian pathogenic Escherichia coli from broiler chickens with colibacillosis

Kim

Yoon²,

Ha³

et al. 2020

Poultry Science

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Avian pathogenic Escherichia coli ( APEC ) causes extensive mortality in poultry flocks, leading to extensive economic losses. The aim of this study was to investigate the phenotypic and genotypic characteristics and antimicrobial resistance of recent APEC isolates. Of the 79 APEC isolates, the most predominant serogroup was O78 (16 isolates, 20.3%), followed by O2 (7 isolates, 8.9%) and O53 (7 isolates, 8.9%). Thirty-seven (46.8%) and six (7.6%) of the isolates belonged to phylogenetic groups D and B2, respectively, and presented as virulent extraintestinal E. coli . Among 5 analyzed virulence genes, the highest frequency was observed in hlyF (74 isolates, 93.7%), followed by iutA (72 isolates, 91.9%) gene. The distribution of the iss gene was significantly different between groups A/B1 and B2/D ( P < 0.05). All group B2 isolates carried all 5 virulence genes. APEC isolates showed high resistance to ampicillin (83.5%), nalidixic acid (65.8%), tetracycline (64.6%), cephalothin (46.8%), and ciprofloxacin (46.8%). The β-lactamases–encoding genes bla TEM-1 (23 isolates, 29.1%), bla CTX-M-1 (4 isolates, 5.1%), and bla CTX-M-15 (3 isolates, 3.8%); the aminoglycoside-modifying enzyme gene aac(3)-II (4 isolates, 5.1%); and the plasmid-mediated quinolone genes qnrA (10 isolates, 12.7%) and qnrS (2 isolates, 2.5%) were identified in APEC isolates. The tetA (37 isolates, 46.8%) and sul2 (20 isolates, 25.3%) were the most prevalent among tetracycline and sulfonamide resistant isolates, respectively. This study indicates that APEC isolates harbor a variety of virulence and resistance genes; such genes are often associated with plasmids that facilitate their transmission between bacteria and should be continuously monitored to track APEC transmission in poultry farms.

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Section: Discussionsupporting

confidence: 87%

Molecular characterization of avian pathogenic Escherichia coli from broiler chickens with colibacillosis

Kim

Yoon²,

Ha³

et al. 2020

Poultry Science

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“…Aggregated European Community data for E. coli isolates from broilers showed that over 50% of isolates were resistant to ciprofloxacin 34 . Moreover, the fluoroquinolones-resistant E. coli typically exhibited clinically significant elevations in MIC values 35 . E. coli isolates resistant to fluoroquinolones are often resistant to other antibiotic groups and genes of virulence 36 .…”

Section: Discussionmentioning

confidence: 99%

Antibiotic resistance and virulence of Escherichia coli strains isolated from animal rendering plant

Gregová

Kmet

2020

Sci Rep

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Processing of animal carcasses and other animal wastes in rendering plants is a significant source of antibiotic resistant microorganisms. The main goal of this study was to investigate the resistance to 18 antibacterial agents including β-lactams, fluoroquinolones, colistin and virulence factors (iss, tsh, cvaC, iutA, papC, kps and ibeA genes) in 88 Escherichia coli strains isolated from a rendering plant over 1 year period. ESBL (Extended-spectrum beta-lactamases) and plasmid-mediated Amp were screened by interpretative reading of MIC. ESBL phenotype was detected in 20.4% of samples and high level of resistance to fluoroquinolone was found in 27.2% of strains. Cephalosporinase CTX-M1, cephamycinase CMY-2, integrase 1 and transposon 3 genes were detected by PCR. Furthermore, there were found three CMY-2 producing E. coli with O25b-ST131, resistant to the high level of enrofloxacin and containing the gene encoding the ferric aerobactin receptor (iutA). One enrofloxacin resistant E. coli strain possessed iss, ibeA, kps and papC virulence genes also with CMY-2, integrase1 and Tn3. ST131 E. coli with CMY-2 has a zoonotic potential and presents a serious health risk to humans.

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“…Previous studies from several countries exhibited the prevalence of qnr genes in veterinary clinical E. coli isolates, which varies in different regions of the world, and revealed that qnr genes in conjugative plasmids confer resistance to quinolones [12,13,15,32,33]. In Algeria, there is no data about PMQR in NAL-resistant E. coli strains isolated from animals, with the exception of one study in humans, which reported a prevalence of 10% [3].…”

Section: Discussionmentioning

confidence: 99%

Occurrence of plasmid-mediated quinolone resistance and virulence genes in avian Escherichia coli isolates from Algeria

Meradi

Barguigua

Nayme

et al. 2017

J Infect Dev Ctries

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Introduction: The emergence and spread of quinolone-resistant Escherichia coli in poultry products puts consumers at risk of exposure to the strains of E. coli that resist antibiotic treatment. The objective of this study was to define the prevalence and virulence potential of poultryassociated nalidixic acid (NAL)-resistant E. coli in the Annaba city, Algeria. Methodology: In total, 33 samples of retail chicken meat were purchased from various butcher shops and examined for bacterial contamination with NAL-resistant E. coli. These isolates were subjected to antimicrobial susceptibility testing and were also investigated for the presence of plasmid-mediated quinolone resistance (PMQR) genes and virulence genes using conventional polymerase chain reaction (PCR) and DNA sequencing. Phylogenetic grouping of the NAL-resistant E. coli isolates was determined by the conventional multiplex PCR method. Results: Twenty-nine (87.8%) products yielded NAL-resistant E. coli. Antibiograms revealed that 96.55% of NAL-resistant E. coli isolates were multidrug resistant (MDR). Resistance was most frequently observed against sulfamethoxazole-trimethoprim (96.6%), tetracycline (96.6%), ciprofloxacin (72%), and amoxicillin (65.5%). Group A was the most prevalent phylogenetic group, followed by groups D, B1, and B2. The PMQR determinants were detected in three isolates with qnrB72 and qnrS1 type identified. Four (13.8%) isolates carried one of the Shiga toxin E. coli-associated genes stx1, stx2, and ehxA alleles. Conclusions: The high prevalence of NAL-resistant E. coli isolated from retail chicken meat with detection of MDR E. coli harboring Shiga toxin genes in this study gives a warning signal for possible occurrence of foodborne infections with failure in antibiotic treatment.

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Prevalence of β-lactam and fluoroquinolone resistance, and virulence factors in Escherichia coli isolated from chickens in Slovakia

Cited by 14 publications

References 50 publications

Molecular characterization of avian pathogenic Escherichia coli from broiler chickens with colibacillosis

Molecular characterization of avian pathogenic Escherichia coli from broiler chickens with colibacillosis

Antibiotic resistance and virulence of Escherichia coli strains isolated from animal rendering plant

Occurrence of plasmid-mediated quinolone resistance and virulence genes in avian Escherichia coli isolates from Algeria

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