Antibiotic resistance and virulence of Escherichia coli strains isolated from animal rendering plant

Abstract

Processing of animal carcasses and other animal wastes in rendering plants is a significant source of antibiotic resistant microorganisms. The main goal of this study was to investigate the resistance to 18 antibacterial agents including β-lactams, fluoroquinolones, colistin and virulence factors (iss, tsh, cvaC, iutA, papC, kps and ibeA genes) in 88 Escherichia coli strains isolated from a rendering plant over 1 year period. ESBL (Extended-spectrum beta-lactamases) and plasmid-mediated Amp were screened by interpretative reading of MIC. ESBL phenotype was detected in 20.4% of samples and high level of resistance to fluoroquinolone was found in 27.2% of strains. Cephalosporinase CTX-M1, cephamycinase CMY-2, integrase 1 and transposon 3 genes were detected by PCR. Furthermore, there were found three CMY-2 producing E. coli with O25b-ST131, resistant to the high level of enrofloxacin and containing the gene encoding the ferric aerobactin receptor (iutA). One enrofloxacin resistant E. coli strain possessed iss, ibeA, kps and papC virulence genes also with CMY-2, integrase1 and Tn3. ST131 E. coli with CMY-2 has a zoonotic potential and presents a serious health risk to humans.

Evaluation of Antibiotic Resistance Pattern of Escherichia coli Isolated From Broiler Chickens With Colibacillosis in Ardabil Province, Iran

“…A study of 83 APEC strains of E. coli in Japan by Ozawa et al 14 also reported the highest resistance to ampicillin (77.1%) followed by oxytetracycline (75.9%) and trimethoprim (25.3%), Enrofloxacin (21.7%) and fluorophenicol (0.6%). According to Gregova and Kmet 19 in Slovakia, the highest percentage of resistance of E. coli isolates in slaughter poultry was related to ampicillin (89%), enrofloxacin (43%), tetracycline (33%) and fluorophenicol (18%). In a study by Khan et al, 20 30 commercial poultry E. coli isolates in Bangladesh reported erythromycin and tetracycline resistance percentages of 100 and 93.3, respectively.…”

“…The findings of the present study are somewhat consistent with those reported in other geophysical regions in terms of drug resistance 15,20 but with the findings of some researchers are different. 19 This discrepancy may be due to differences in the type, amount and persistence of antimicrobial compounds use, and temporal and spatial differences and differences in the isolates studied. Multiple drug resistance to antibiotic compounds is a major problem and a common phenomenon seen in the majority of E. coli strains of the poultry pathogen in which bacterial isolates are resistant to at least two types of antibiotics.…”

Efficient Addition of Waste Glass in MK-Based Geopolymers: Microstructure, Antibacterial and Cytotoxicity Investigation

“…In order to provide preliminary functional information of GP and GP/WG geopolymers, the latter were tested for their antibacterial efficacy at 7, 14 and 28 days curing time using two different strains of bacteria: a Gram-negative and a Gram-positive. The incubation temperatures, 44 and 36 °C for E. coli and E. faecalis , respectively, were chosen to allow the faster growth of the two different bacteria, differing from the experiments run by Mejía-de Gutiérrez et al 2020 [ 48 ] when evaluating surface properties at room temperature. Figure 7 shows representative data achieved for GP and GP/WG 60% after 28 days curing time for 37–53 and 75–105 µm grain sizes.…”

New Insight on Antibiotic Resistance and Virulence of Escherichia coli from Municipal and Animal Wastewater

“…In wastewater samples from the rendering plant, we formerly detected a high level of resistance to fluoroquinolones, betalactams, CTX-M1, CMY-2, Int 1, and Tn 3 genes. In one sample, the presence of a pandemic clone of E. coli ST131 with CMY-2 was present [ 31 ].…”