Prevalence of Seed Borne Fungi Associated with Chickpea Seeds and Biological and Chemical Control of Fusarium oxysporum Causing Wilt Disease

Abstract

A total of 20 chickpea seed samples were collected from BARI, Gazipurand different locations of Savar, Dhaka district. Blotter method was used for detection of the associated fungi of chickpea seeds. Altogether 14 fungi comprising 12 genera namely Aspergillus flavus, Aspergillus niger, Aspergillus sp, Botryodiplodia theobromae, Botryti cinerea, Curvularia lunata, Chaetomium globosum, Cladosporium sp., Colletotrichum dematium, Fusarium oxysporum, Macrophomina phasaelina, Rhizoctonia solani, Rhizopus stolonifer and Stemphylium sarciniforme were isolated from chickpea seed samples. Among the fungi isolated fungi, F. oxysporumwas most prevalent with an average incidence of 18.95% and found in all the seed samples. The germination of seed samples varied from 55-90 % on blotter. The pathogenic fungi and other storage fungi like Aspergillus caused lower the germination of the seeds.A pathogenicity test was conducted with 20 isolates of F. oxysporum against their origin of chickpea seed samples in pot culture. The pathogenecity ranged from weak to highly pathogenic. The isolates FO 19, FO 17, FO 11 and FO 18 were highly virulent. The isolates FO 9, FO10 and FO 15 were virulent and rest of the isolates were moderately virulent. The isolates FO 2 and FO 3 were weak pathogen.J. Environ. Sci. & Natural Resources, 10(2): 45-54 2017

Application of clove and dill oils as an alternative of salphos for chickpea food seed storage

“…and Rhizopus nigricans 28 ; seven fungal species such as Alternaria alternata, Aspergillus flavus, A. niger, A. fumigatus, Curvularialunata, Fusarium monoliforme and Rhizoctonia solani 29 ; Thirteen different fungi like Actinomucor repens, Alternaria alternata, Aspergillus flavus, A. fumigatus, A. niger, A. ochraceus, Cladosporium sp., Fusarium oxysporum, Fusarium sp., Mucor varians, Penicillium notatum, Phomaherbarum, Rhizopus stolonifer 30 ; Aspergillus niger (83%), Fusarium solani (79%), Aspergillus flavus (75%), Alternaria alternata (60%) and Aspergillus nidulans (57%). Minimum seed borne fungi were reported to be Rhizopus stolonifer , and Fusarium oxysporum 31 ; 14 fungi comprising 12 genera namely Aspergillus flavus, Aspergillus niger , Aspergillus sp, Botryodiplodiatheobromae , Botryti cinerea , Curvularialunata , Chaetomium globosum, Cladosporium sp., Colletotrichum dematium, Fusarium oxysporum , Macrophomina phasaelina , Rhizoctonia solani , Rhizopus stolonifer and Stemphylium sarciniforme 32 ; Nine fungal species namely Alternaria alternata (Fr.) Keissl., Aspergillus flavus Link, Aspergillus niger van Tieghem, Aspergillus oryzae (Ahlburg) E. Cohn, Fusarium oxysporum Schlecht.…”