Prevalence of Parvovirus B19 and Hepatitis A virus in Portuguese blood donors

Henriques, Isabel; Monteiro, Fátima; Meireles, Elsa; Cruz, Aldina; Tavares, Gilberto; Ferreira, Mariana; Araújo, Fernando

doi:10.1016/j.transci.2005.06.002

Cited by 21 publications

(13 citation statements)

References 10 publications

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“…Generally, solid organs or stem cell recipients are at increased risk for viral reactivation that may originate from a persisting previous viral infection or from transmitted pathogens through the transplantation [7]. Due to notable tropism of B19 to human bone marrow and erythroid precursor cells for replication, anemia is considered the main complication of B19 infection [8]. B19 infection can also cause aplastic crisis in patients with chronic hemolytic anemia.…”

Section: Introductionmentioning

confidence: 99%

Frequency and Genotype of Human Parvovirus B19 among Iranian Hemodialysis and Peritoneal Dialysis Patients

Sharif

Aghakhani²,

Velayati³

et al. 2016

Intervirology

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Objectives: The aim of this study was to evaluate the frequency and genotype of human parvovirus B19 and its relation with anemia among Iranian patients under dialysis. Methods: Fifty hemodialysis (HD) and 33 peritoneal dialysis (PD) patients were enrolled. B19 IgG and IgM antibodies were assessed by ELISA, and the presence of B19 DNA was evaluated by nested PCR. PCR products were sequenced directly and phylogenetic analysis was performed. Results: In the HD group, the prevalence of B19 antibodies was 54% for IgG and 4% for IgM. B19 DNA was detected in 10% of the cases, and 10% showed B19 IgG and viremia simultaneously. In the PD group, the prevalence of B19 IgG and IgM was 57.6 and 0% respectively, whereas B19 DNA was found in 12.1% of the group. A total of 9.1% showed B19 IgG and viremia concurrently. There was no significant difference regarding anemia and B19 infection in either group. All B19 isolates were clustered in genotype 1A. Conclusion: Our findings indicate that B19 infection plays no role in leading chronic anemia in dialysis patients. However, persistent B19 viremia and the circulation of the same strains in dialysis patients may indicate a potential risk for the contamination of dialysis equipment and nosocomial spread of B19 infection within dialysis units.

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Section: Introductionmentioning

confidence: 99%

Frequency and Genotype of Human Parvovirus B19 among Iranian Hemodialysis and Peritoneal Dialysis Patients

Sharif

Aghakhani²,

Velayati³

et al. 2016

Intervirology

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“…The virus is transmitted effectively by close contact or blood transfusion. The prevalence of specific IgG antibodies against B19V increases with advancing age: while it is about 15% in children, it increases to approximately 30–60% in adults and it exceeds 85% in the elderly population [Henriques et al, 2005].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and association of human parvovirus B19V with hepatitis B and C viruses in Nigeria

Opaleye¹,

Fagbami²,

Lalremruata³

et al. 2011

J. Med. Virol.

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Background In the course of setting up the National Mesothelioma Surveillance Program (PNSM), established in France in 1998, the question arose as to the most suitable method of assessing occupational exposure. The aim of this study was to define the most suitable rating method for assessing occupational asbestos exposure in order to assess medico‐social care. Method The study included 100 subjects—50 cases of mesothelioma and 50 controls—randomly selected and representing 457 jobs held. Job asbestos exposure was assessed by a six‐expert panel using two methods: “by job” rating, where all the jobs in were assessed regardless of the subjects; and “by subject” rating, where all the jobs of a subject were assessed at the same time. Consensus was obtained and subjects' exposure was calculated for each rating. Then, two internal experts assessed job asbestos exposure with the “by subject” rating. Kappa coefficients were used to measure agreement between the ratings. Results Agreement between “by job” and “by subject” ratings was very good for subject probability of exposure (kappa = 0.84) and cumulative exposure index (kappa = 0.80). Agreement between the six‐expert panel and the two internal experts was good for subject exposure (kappa for probability = 0.71; kappa for cumulative exposure index= 0.68). Conclusion This study shows that the two rating systems have good or very good agreement. These results validate the routine use in the PNSM of the “by subject” rating, with the advantage of being convenient and quick to provide feedback on occupational asbestos exposure to mesothelioma cases for compensation. Am. J. Ind. Med. 55:440–449, 2012. © 2012 Wiley Periodicals, Inc.

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“…The 13 B19V DNA-negative samples (including the 2 negative borderline samples) showed normal (low) neopterin levels as expected, but the 11 (85%) IgG-positive samples correlate with the levels found in the general population, where approximately 80% of the adult population is IgG anti-B19V-positive [44]. IgG is present in the fractionation pools where it may be helpful in neutralizing the small B19V concentrations that could be present [5,45].…”

Section: Discussionmentioning

confidence: 77%

Characterization of Markers of the Progression of Human Parvovirus B19 Infection in Virus DNA-Positive Plasma Samples

Bonjoch¹,

Obispo²,

Alemany³

et al. 2015

Transfus Med Hemother

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Background: Accurate characterization of the infection stage in parvovirus B19(B19V)-positive plasma donations would help establish the donation deferral period to contribute to a safe fractionation pool of plasma. Methods: Viral DNA load of 74 B19V DNA-positive plasma samples from whole blood donations was determined by titration using nucleic acid testing. Markers of cellular (neopterin) and humoral (B19V-specific IgM and IgG) immune response were determined by ELISA in 32 B19V DNA-positive samples and in 13 B19V DNA-negative samples. The infection progression profile was estimated according to B19V DNA load and the presence of immune response markers. Results: B19V DNA load in the 74 samples was 10⁶-10¹³ IU/ml. The distribution of 14 out of 32 selected B19V DNA-positive samples plus 2 B19V DNA-negative samples with no immune response marker followed along an upward curve according to B19V DNA load. After the peak, the distribution of 18 immune marker-positive samples followed along a downward curve according to their B19V DNA load and was grouped as follows: neopterin (n = 4), neopterin+ IgM (n = 8), neopterin + IgM + IgG (n = 3), IgM + IgG (n = 2), IgM (n = 1). There were 11 B19V DNA-negative IgG-positive samples. Conclusion: This study of B19V-DNA load and levels of neopterin, IgM, and IgG allows for reliable characterization and distribution into the different stages of B19V infection.

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Prevalence of Parvovirus B19 and Hepatitis A virus in Portuguese blood donors

Cited by 21 publications

References 10 publications

Frequency and Genotype of Human Parvovirus B19 among Iranian Hemodialysis and Peritoneal Dialysis Patients

Frequency and Genotype of Human Parvovirus B19 among Iranian Hemodialysis and Peritoneal Dialysis Patients

Prevalence and association of human parvovirus B19V with hepatitis B and C viruses in Nigeria

Characterization of Markers of the Progression of Human Parvovirus B19 Infection in Virus DNA-Positive Plasma Samples

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