Frequency and Genotype of Human Parvovirus B19 among Iranian Hemodialysis and Peritoneal Dialysis Patients

Sharif, Alireza; Aghakhani, Arezoo; Velayati, Ali Akbar; Banifazl, Mohammad; Sharif, Mohammad Reza; Razeghi, Effat; Kheirkhah, Davood; Kazemimanesh, Monireh; Bavand, Anahita; Ramezani, Amitis

doi:10.1159/000455124

Cited by 11 publications

(9 citation statements)

References 35 publications

(41 reference statements)

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“…Genotype 1 is the most common genotype throughout the world and other genotypes were detected rarely . B19‐1A was the only detectable genotype in our study which is compatible with those of previous reports in Iran on other settings such as HIV patients, dialysis cases, and normal population …”

Section: Discussionsupporting

confidence: 92%

Seroprevalence, molecular epidemiology and quantitation of parvovirus B19 DNA levels in Iranian blood donors

Zadsar

Aghakhani

Banifazl

et al. 2018

Journal of Medical Virology

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Human parvovirus B19 (B19) infection is common among blood donors, and healthy blood donors can transmit virus via transfusion. Due to resistance of B19 to viral inactivation methods, there is a potential concern regarding transfusion safety in blood products. We aimed to determine the seroprevalence, molecular epidemiology, and quantitation of B19 DNA levels in blood donors in Tehran, Iran. A total of 500 blood donors from Blood Transfusion Research Center were studied. ELISA was used for detection of B19 IgG and IgM and nested PCR was carried out for detection of B19 DNA. PCR products were subjected to direct sequencing. B19 viral load was determined by real time PCR. B19 IgG, IgM, and DNA were detected in 27.6, 2.6, and 1.2% of donors respectively. Ten samples (2%) were positive for both antibodies while in four cases (0.8%), B19 IgG and DNA detected simultaneously. One case had B19 IgM, IgG, and viremia concurrently. The titers of B19 DNA in four of six donors were more than 10 IU/mL (high level viremia) and all four cases had IgG simultaneously. All B19 isolates categorized in genotype 1A. Our findings indicated that prevalence of B19 DNA in Iranian blood donors was comparable with previous studies throughout the world. High level B19 viremia found in 0.8% of our donors and all viremic donors revealed neutralizing B19 antibody. Therefore implementation of a B19 screening test for each volunteer blood donor does not appear to be necessary but B19 testing for plasma-derived products seems important in Iranian donors.

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Section: Discussionsupporting

confidence: 92%

Seroprevalence, molecular epidemiology and quantitation of parvovirus B19 DNA levels in Iranian blood donors

Zadsar

Aghakhani

Banifazl

et al. 2018

Journal of Medical Virology

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“…Previous studies conducted in Iran found B19V DNA in 10% (5/ 50) of CKD patients (Sharif et al, 2016) and in 13.1% (13/99) of those infected with HIV (Azadmanesh et al, 2015). In a single Brazilian Figure 2.…”

Section: Discussionmentioning

confidence: 87%

“…Nevertheless, there are several reasons to think that parvovirus may be an important pathogen in these populations, since anemia is a common consequence of B19V infection and is also a predictable consequence of chronic renal insufficiency (Kazmi et al, 2001). Although the prevalence of severe anemia is high at the onset of dialysis, and the early presence of anemia in end-stage renal disease (ESRD) is associated with a greater rate of subsequent hospitalization and mortality (Ma et al, 1999), many studies have not found a significant association between anemia and B19V DNA detection in dialysis patients (Guiserix et al, 1996;Sharif et al, 2016;Alves et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

High prevalence of parvovirus B19 infection in patients with chronic kidney disease under hemodialysis: A multicenter study

Alves

Langella

Barbosa

et al. 2020

International Journal of Infectious Diseases

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Objectives: Parvovirus B19 (B19V) infection is commonly acute and self-limited, but in chronic kidney disease (CKD) patients under dialysis treatment, this infection could increase susceptibility to acute and chronic anemia. The aim of this study was to evaluate the frequency and risk of B19V infection among Brazilian CKD patients under dialysis. Methods: A study was conducted among 221 CKD patients and a control group of 142 blood donors. B19V infection was evaluated in serum samples by real-time PCR, and ELISA (anti-B19V IgM and IgG). Results: B19V DNA was detected in 65% (145/221) of CKD patients, which was significantly higher (p < 0.001) than in the blood donors (6.3%). Simultaneous detection of B19V IgG and viremia was shown in 40.3% of CKD patients, which was indicative of persistent B19V infection. CKD patients showed an increased risk of developing B19V infection (OR = 28.1, CI = 13.5-58.5, p = 0.001). Conclusions: Despite an absence of clinical signs of B19V infection, these data highlight the importance of B19V infection in this high-risk population, since a persistent B19V infection could become clinically significant after renal transplant. Moreover, the persistent viremia should be considered as a potential risk, mainly because of the contamination of dialysis equipment.

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“…In Iran and Brazil, the prevalence of parvovirus B19 IgG antibodies among hemodialysis patients was 54% and 67.5%, respectively [23,24], while, in Iraq, a high seroprevalence of 90.2% was observed [25].…”

Section: Discussionmentioning

confidence: 99%

Parvovirus B19 in Croatia: A Large-Scale Seroprevalence Study

et al. 2021

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Background and Objectives: Seroepidemiological studies indicate that parvovirus B19 circulates in all areas of the world, although with some differences. The aim of this study is to analyze the seroprevalence of parvovirus B19 in the Croatian population. Materials and Methods: From 2010 to 2021, 1538 serum samples from different populations were tested for the presence of parvovirus B19 IgM/IgG antibodies. Serological tests were performed using a commercial enzyme-linked immunosorbent assay. Results: IgG antibodies were detected in 986/64.1% of participants with differences (p < 0.001) among the following population groups: 42.4% of children and adolescents, 67.1% of the adult general population, 66.7% of hemodialysis patients, and 65.6% of liver transplant recipients. Seroprevalence increased with age, from 30.0% in the 6 months–9 years age group to 69.0% in the 40–49 years age group, and remained stable thereafter (68.8–73.3%). There was no difference in the seropositivity among males (66.1%) and females (63.1%), as well as the place of residence (suburban/rural 63.9%, urban 64.1%). IgM antibodies (current/recent infection) were found in 61/4.0% of participants with the highest seropositivity in the youngest age group (11.1%). In pregnant women, seroprevalence was higher in women with an unfavorable obstetric history compared with a normal pregnancy (IgG 71.0% vs. 62.6%; IgM 6.5% vs. 2.4%), but these differences were not significant. Logistic regression showed that the adult population had almost three times higher risk of IgG seropositivity compared to children/adolescents (general population OR = 2.777, 95% CI = 2.023–3.812; hemodialysis patients OR = 2.586, 95% CI = 1.531–4.367; and transplant patients OR = 2.717, 95% CI = 1.604–4.603). A one-year increase in age increased the risk of IgG seroprevalence (OR = 1.017; 95% CI = 1.011–1.022). Conclusions: Older age was the main risk factor for IgG seropositivity. Hemodialysis and organ transplantation seem unrelated to the increased parvovirus B19 seroprevalence. The role of parvovirus B19 in the etiology of TORCH infections needs to be studied further.

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Frequency and Genotype of Human Parvovirus B19 among Iranian Hemodialysis and Peritoneal Dialysis Patients

Cited by 11 publications

References 35 publications

Seroprevalence, molecular epidemiology and quantitation of parvovirus B19 DNA levels in Iranian blood donors

Seroprevalence, molecular epidemiology and quantitation of parvovirus B19 DNA levels in Iranian blood donors

High prevalence of parvovirus B19 infection in patients with chronic kidney disease under hemodialysis: A multicenter study

Parvovirus B19 in Croatia: A Large-Scale Seroprevalence Study

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