Prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli in Dutch dairy herds

Heuvelink, Annet; Gonggrijp, Maaike; Buter, Rianne; Bogt-Kappert, C C Ter; Schaik, G. van; Velthuis, A.G.J.; Lam, T.J.G.M.

doi:10.1016/j.vetmic.2019.04.005

Cited by 21 publications

(17 citation statements)

References 28 publications

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“…coli from food-producing animals, raising worries about animal and public health (Seiffert, Hilty, Perreten, & Endimiani, 2013). This study determines an overall 75.58% of ESBLproducing E. coli from bovine mastitis, which is higher than the previous reports of ESBL from bovine mastitis in China and other parts of the world (Ali et al, 2017;Heuvelink et al, 2019;Kar et al, 2015;Zheng et al, 2019). The overall incidence of ESBL-producing E. coli isolates was much greater in Farm B (89.09%), followed by Farm C (75.18%), and Farm A (58.59%).…”

Section: Discussionmentioning

confidence: 57%

Characterization and virulence factors distribution of blaCTX-M and mcr-1 carrying Escherichia coli isolates from bovine mastitis

Shafiq¹,

Huang

Rahman

et al. 2020

Preprint

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Escherichia coli is considered as one of the critical pathogens causing contagious mastitis. The aim of the current study was to investigate the occurrence of ESBL and colistin resistant E. coli and its genotypic characterization and identification of virulence determinants in cases of bovine mastitis in Jiangsu Province, China. Five hundred and thirty-five (535) milk samples presenting mastitis symptoms were collected from three dairy farms. Susceptibility testing was identified by micro-dilution method. E. coli isolates were used to detect ESBL genes (blaCTX-M, blaSHV and blaTEM) as well as COL-resistant genes mcr-(1-9). Multiplex PCR approach was used for the detection of major CTX-M groups, different phylo-groups, and virulence genes. The clonal relationship of the isolates was then evaluated with MLST, MLSA and PFGE. Multi-drug resistance (MDR) was found in more than 85% of ESBL and/or COL resistant E. coli isolates. Genotypic characterization indicated a dominance of CTX-M-1 group, and the most prevalent alleles observed in this study were blaCTX-M-28 (38.37%), blaCTX-M-14 (17.44%), blaCTX-M-66 (13.95%), blaCTX-M-55 (10.46%), and blaCTX-M-69 (6.97%), blaCTX-M-15 (4.65%), blaCTX-M-177 (4.65%), and blaCTX-M-148 (3.48%). The results confirmed that group A was the most predominant group with 54.73%, followed by group B1 and B2 (24.21%, 10.52%) respectively. The targeted virulence genes were detected in 97.89% (n=93) isolates. All isolates belong to group A, B1, B2, C, D, and E showed the presence of virulence factors. Among virulent genes ompC, was detected in 97.89% (n=93) isolates followed by fimH in 61.05% (n=58), ECs3703 57.89% (n=55), ompF 50.52% (n=48). MLST and PFGE of the 22 E. coli isolates were determined for clonal relatedness and yielded clear fingerprint patterns. Majority of the E. coli isolates carrying ESBL and mcr-1 isolates were clonally unrelated. Sequence types 58 and ST410 were the most predominant comprising 2 each (2/20=20%).

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Section: Discussionmentioning

confidence: 57%

Characterization and virulence factors distribution of blaCTX-M and mcr-1 carrying Escherichia coli isolates from bovine mastitis

Shafiq¹,

Huang

Rahman

et al. 2020

Preprint

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“…Escherichia coli Europe Germany Faecal (Wieler et al, 2011) Mastitis (Freitag et al, 2017;Michael et al, 2017) Diarrheic (Ewers et al, 2014) Sick (Michael et al, 2017) Infection (Brennan et al, 2016) Denmark Commensal (Kjeldsen et al, 2015) ND (Garcia-Fernandez et al, 2011;Jakobsen et al, 2015) Spain Mastitis (Briñas et al, 2005) Finland Faecal (P€ aiv€ arinta et al, 2016) France Faecal (Haenni et al, 2014;Hartmann et al, 2012;Madec et al, 2008;Meunier et al, 2006) Mastitis (Dahmen et al, 2013) Diarrheic (Hartmann et al, 2012) Sick (Madec et al, 2008;Valat et al, 2016) Infection (Meunier et al, 2006) Hungary Infection (Toth et al, 2013) Portugal Faecal (Ramos et al, 2013) Mayotte ND (Gay et al, 2018) Netherlands Faecal (Ceccarelli et al, 2019;Heuvelink et al, 2019;Hordijk et al, 2013a, b, c) United Kingdom…”

Section: Ctx-m-1mentioning

confidence: 99%

“…Faecal (Velasova et al, 2019) Infection (Hunter et al, 2010) ND (Stokes, 2014) Czech Republic Faecal (Dolejska et al, 2011b) Sick (Dolejska et al, 2013) R eunion ND (Gay et al, 2018) Slovakia Faecal (Kme t and Buj n akov a, 2018) Sweden Faecal (Duse et al, 2015) Switzerland Faecal (Endimiani et al, 2012;Geser et al, 2012a;Zurfluh et al, 2015) Turkey Faecal (AslantaŞ et al, 2017;Pehlivanoglu et al, 2016) North America Canada ND (Cormier et al, 2019) USA Faecal (Mir et al, 2016;Mollenkopf et al, 2012;Wittum et al, 2010) Asia China Mastitis (Ali et al, 2016(Ali et al, , 2017 South Korea Mastitis (Tark et al, 2017) Indonesia Faecal (Sudarwanto et al, 2016) Japan Mastitis (Ohnishi et al, 2013b) Klebsiella pneumoniaeEurope Italy Mastitis (Locatelli et al, 2010) Klebsiella ozaenae Italy Faecal (Stefani et al, 2014) Salmonella enterica Germany ND (Rodríguez et al, 2009) CTX-M-1/61 E. coli Europe Germany Faecal (Dahms et al, 2015) CTX-M-2 E. coli South America Brazil Faecal (Palmeira et al, 2018) Europe Germany Mastitis (Eisenberger et al, 2017;Freitag et al, 2017;Michael et al, 2017) Sick (Michael et al, 2017) Netherlands Faecal (Ceccarelli et al, 2019;Heuvelink et al, 2019) North America Canada Faecal (Cormier et al, 2016) Asia Japan Faecal (Shiraki et al, 2004) Diarrheic (Ohnishi et al, 2013a) Infection (Asai et al, 2011) K. pneumoniae Japan Mastitis (Ohnishi et...…”

Section: Ctx-m-1mentioning

confidence: 99%

“…Infection (Hunter et al, 2010) Turkey Faecal (AslantaŞ et al, 2017;Pehlivanoglu et al, 2016) Asia China Faecal (Zheng et al, 2019) Mastitis (Ali et al, 2016(Ali et al, , 2017 South Korea Mastitis (Tark et al, 2017) Hong (Eisenberger et al, 2017;Freitag et al, 2017;Michael et al, 2017) Sick (Michael et al, 2017) Belgium Faecal (Pardon et al, 2017) Infection (Pardon et al, 2017) France Faecal (Haenni et al, 2014;Madec et al, 2008) Mastitis (Dahmen et al, 2013) Sick (Madec et al, 2008) Wales Sick (Tyrrell et al, 2016) Netherlands Faecal (Ceccarelli et al, 2019;Heuvelink et al, 2019;Hordijk et al, 2013a, b, c) United Kingdom Faecal (Cottell et al, 2011;Horton et al, 2011;Randall et al, 2014;Snow et al, 2011) Infection (Hunter et al, 2010) ND (Stokes, 2014;Stokes et al, 2013) Switzerland Faecal (Geser et al, 2012a;Zurfluh et al, 2015) Mastitis (Geser et al, 2012a) Asia China Faecal (Zheng et al, 2012(Zheng et al, , 2019 Mastitis (Ali et al, 2016(Ali et al, , 2017 South Korea Faecal (Rayamajhi et al, 2011;Tamang et al, 2013a) Sick (Lim et al, 2009) Hong Kong Faecal Japan Mastitis (Ohnishi et al, 2013b) Diarrheic (Ohnishi et al,...…”

Section: Ctx-m-1mentioning

confidence: 99%

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Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Palmeira

Ferreira

2020

Heliyon

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Food producing animal is a global challenge in terms of antimicrobial resistance spread. Extended-spectrum betalactamase (ESBL)-producing Enterobacteriaceae are relevant opportunistic pathogens that may spread in many ecological niches of the One Health approach as human, animal and environment due to intestinal selection of antimicrobial resistant commensals in food production animals. Cattle production is a relevant ecological niche for selection of commensal bacteria with antimicrobial resistance from microbiota. Enterobacteriaceae show importance in terms of circulation of resistant-bacteria and antimicrobial resistance genes via food chain creating a resistance reservoir, setting up a threat for colonization of humans and consequent health risk. ESBL-producing Enterobacteriaceae are a threat in terms of human health responsible for life threatening outbreaks and silent enteric colonization of community populations namely the elder population. Food associated colonization is a risk difficult to handle and control. In a time of globalization of food trading, population intestinal colonization is a mirror of food production and in that sense this work aims to make a picture of ESBL-producing Enterobacteriaceae in animal production for food over the world in order to make some light in this reality of selection of resistant threats in food producing animal.

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“…For this reason, the World Health Organization has included ESBL-and/or AmpCproducing strains of these bacteria in the list of "alert pathogens" (3, 10). Due to the fact that genes responsible for the production of these enzymes are often located on mobile genetic elements, such as plasmids, their dissemination in the environment is easy and rapid (13,15,20,26,32). Many authors have suggested a possible transfer of pathogenic, resistant bacteria from animals to humans via food chain or by contact with infected individuals (2,3,5,8,12,25,26).…”

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confidence: 99%

Occurrence of extended-spectrum beta-lactamaseand AmpC-type cephalosporinase-producing Escherichia coli in mastitic cow’s milk

Różańska¹,

Kubajka²,

Weiner³

2021

Medycyna Weterynaryjna

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The aim of the study was to evaluate the occurrence of Escherichia coli producing extended-spectrum betalactamases (ESBL and/or AmpC) in the milk of cows with mastitis. A total 2,500 milk samples from mastitic cows were tested in 2014-2018. The investigations included the culture of bacteria on MacConkey agar with cefotaxime, identification with the API Rapid 32 E test, synergy disc test D68C, assessment of antimicrobial resistance by the microdilution method and confirmation of the occurrence of genes encoding ESBL and AmpC. Out of 133 isolates identified as E. coli, 87 were recognized as ESBL producers and 46 as chromosomally encoded cephalosporinase AmpC producers. The blaTEM was predominant in the ESBL producers. All 46 AmpC-positive strains had the blaCMY gene. The results confirmed the occurrence of extended-spectrum betalactamase- producing E. coli in inflammatory secretions from mastitic

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Prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli in Dutch dairy herds

Cited by 21 publications

References 28 publications

Characterization and virulence factors distribution of blaCTX-M and mcr-1 carrying Escherichia coli isolates from bovine mastitis

Characterization and virulence factors distribution of blaCTX-M and mcr-1 carrying Escherichia coli isolates from bovine mastitis

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Occurrence of extended-spectrum beta-lactamaseand AmpC-type cephalosporinase-producing Escherichia coli in mastitic cow’s milk

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