Prevalence characteristics of high-risk human papillomaviruses in women living in Shanghai with cervical precancerous lesions and cancer

Gu, Ying; Ma, Chenyun; Zou, Jun; Zhu, Yi; Rong, Yang; Xu, Yixin; Zhang, Yu

doi:10.18632/oncotarget.8262

Cited by 22 publications

(27 citation statements)

References 30 publications

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“…In present study, HPV 58 is a dominant high-risk genotype and the prevalence of HPV58 in patients with cervicitis, CIN I, CIN II/III and invasive SCC was 9.09%, 21.28%, 25.40 and 20.49%, respectively. Although the overall prevalence rate of HPV58 in our research is very higher than the reports of Japanese and world, in which HPV58 prevalence rates are 7.0% in patients with CIN II/III and 3.3% ~ 13.3% in patients with SCC [10, 37], our result is consistent with some recent Chinese reports [33, 38], where 16.4%, 20.1%, 23.5% and 31.4% patients with cervicitis, CIN I, CIN II/III and invasive SCC and 29.1% and 24.3% patients with CIN II/III and invasive SCC showed HPV58 infection.…”

Section: Discussionsupporting

confidence: 89%

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Human papillomavirus genotypes associated with cervical precancerous lesions and cancer in the highest area of cervical cancer mortality, Longnan, China

Guo

Wang²,

et al. 2017

Infect Agents Cancer

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BackgroundThe mortality of cervical cancer in Longnan is as high as 39/10 million, ranking first in China.MethodsBetween 2012 to 2016, 329 samples with cervicitis, cervical intraepithelial neoplasia grade 1 to 3 (CINI to III), and invasive squamous cell carcinoma (SCC) were collected. HPV genotypes were examined with a validated kit for 23 different HPV subtypes.ResultsCompared to cervicitis, the HPV positivity is significantly higher in CINI, CIN II/III, and SCC (38.60%, 74.60%, 87.50% and 89.05%, P < 0.001) and the positivity is also higher in SCC compared to CINI (P < 0.01). The most frequently detected genotypes were HPV16 in cervicitis, HPV16, 58 and 52 in CINI and CIN II/III, and HPV16, 58 and 18 in SCC groups. HPV16 positivity in cervicitis, CINI, CIN II/III, and SCC patients were 45.46%, 46.81%, 60.32% and 78.69%, respectively. Compared to cervicitis and CINI, the odds ratios (OR) for SCC in HPV16 positive patients were 2.96 (95% confidence interval [CI]: 1.09–8.00, P < 0.05) and 4.20 (95% confidence interval [CI]: 2.05–8.61, P < 0.001), respectively. In addition, the multiple infections in cervicitis, CINI, CINII/III and SCC group are 9.09%, 27.66%, 26.98% and 25.41% and HPV16 + 58 was the most common combinations.ConclusionThese findings highlight the key role of HPV16, 58, 52 and 18 in the development of CIN and SCC in Longnan women and a fully aware of regional differences in HPV genotype distribution are tasks for cervical cancer control and prevention.

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Section: Discussionsupporting

confidence: 89%

“…The distribution of dominant HPV genotypes showed obvious regional differences [33]. HPV18 is reported to be one of the two most carcinogenic HPV genotypes (HPV16 and 18), accounting for 10 ~ 15% of cervical cancers [11, 24, 32].…”

Section: Discussionmentioning

confidence: 99%

Human papillomavirus genotypes associated with cervical precancerous lesions and cancer in the highest area of cervical cancer mortality, Longnan, China

Guo

Wang²,

et al. 2017

Infect Agents Cancer

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“…In our population, the HPV 18 prevalence rates in patients with cervicitis, CIN1, CIN2‐3 and invasive SCC were 11.6%, 10.9%, 8.3%, and 11.8%, respectively. Because the patients in the study were at high risk of cervical cancer and received colposcopies due to abnormal cervical screening results, the infection rates among patients with cervicitis and CIN1 were relatively higher than those in other reports . Another reason was that as many as 66.6% (513/770) of the patients with cervicitis and 62.0% of the patients (245/395) with CIN1 received a colposcopy and cervical biopsy due to slight abnormalities, which included simple HPV 16/18‐positive patients and HPV‐positive ASC‐US.…”

Section: Discussionmentioning

confidence: 54%

“…Because the patients in the study were at high risk of cervical cancer and received colposcopies due to abnormal cervical screening results, the infection rates among patients with cervicitis and CIN1 were relatively higher than those in other reports. 46 Another reason was that as many as 66.6% ( Asia. 47 In our study, the HPV 52 prevalence rates in patients with cervicitis, CIN1, CIN2-3, and invasive SCC were 15.2%, 14.4%, 16.8%, and 3.9%, respectively.…”

Section: Ors For Hpv 16 Infectionsmentioning

confidence: 99%

Prevalence characteristics of single and multiple HPV infections in women with cervical cancer and precancerous lesions in Beijing, China

et al. 2018

Journal of Medical Virology

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We assessed the prevalence characteristics of single and multiple high‐risk human papillomavirus (HR‐HPV) infections. A total of 1783 women who underwent colposcopy and cervical biopsy for abnormal ThinPrep Cytology Test and/or HR‐HPV subtype genotyping results were enrolled in the study. Among the participants, 770 were diagnosed with cervicitis, 395 with cervical intraepithelial neoplasia grade 1 (CIN1), 542 with CIN2‐3, and 76 with squamous cell carcinoma (SCC), with HR‐HPV infection rates of 75.8%, 85.8%, 95.9%, and 88.4%, respectively. The prevalence of total and multiple HR‐HPV infections exhibited a bimodal age distribution with a peak at ≤25 years, a decline with age and a second peak at ≥55 years, whereas single HR‐HPV infections exhibited one peak from 35 to 44 years. The four most dominant HPV genotypes were HPV 16 (29.5%), 52 (15.0%), 58 (14.2%), and 18 (10.4%). In total, 67.0%, 70.4%, and 82.1% of patients with CIN1, CIN2‐3, and SCC, respectively, had a single HR‐HPV infection, which increased significantly with the aggravation of the cervical lesion grade (P = 0.045). Patients with a single HPV 16 infection had higher incidences of CIN2+ (62.2%) than those with multiple HPV 16 infections (52.4%) (P = 0.021). Patients coinfected with HPV 16 had higher CIN2+ incidence than those with single HPV 52, 31, 33, 35, 39, 45, 51, 56, or 59 infections (P < 0.001). This study provided baseline data on the prevalence characteristics of single and multiple HR‐HPV infections in women attending a gynecological outpatient clinic in Beijing.

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“…It has been found in 11.5 to 28% of cervical cancer patients in China, 16% in Korea and 8% in Japan [15]. In a recent study that evaluated the prevalence of high-risk HPVs in cervical intraepithelial lesions in Shanghai showed that the three most dominant HPV genotypes were HPV16, 58, and 52 [16]. HPV58 is the third most common HPV type in Eastern Asia overall [17].…”

Section: Discussionmentioning

confidence: 99%

Production of Polyclonal Antibody to the HPV58 E7 Protein and Its Detection in Cervical Cancer

et al. 2016

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The persistent infection of high-risk human papillomavirus (HPV) is one of the most common causes of cervical cancer worldwide, and HPV type 58 is the third most common HPV type in eastern Asia. The E7 oncoprotein is constitutively expressed in HPV58-associated cervical cancer cells and plays a key role during tumorigenesis. To study the biological function of HPV58 E7 and to characterize E7 protein-host cell interactions, we cloned the human HPV58 E7 gene and produced specific E7 antibodies. The HPV58 E7 gene was cloned into a prokaryotic expression vector, pGEX-4T2. The recombinant plasmid pGEX-4T2-(HPV58-E7) was transformed into Escherichia coli DH5α and expressed as a fusion protein containing a GST tag. After purification and removal of the GST affinity tag, the E7 protein was used as an antigen for the production of antiserum in rabbits. The specificity of the purified HPV58 E7 antibody was detected by western blotting, immunofluorescence and immunohistochemistry analysis. These methods demonstrated that the polyclonal antibody could specifically recognize the endogenous and the recombinant HPV58 E7 proteins. Immunohistochemistry analysis indicated that the E7 protein was localized in the nucleus of cervical cancer cells.

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Prevalence characteristics of high-risk human papillomaviruses in women living in Shanghai with cervical precancerous lesions and cancer

Cited by 22 publications

References 30 publications

Human papillomavirus genotypes associated with cervical precancerous lesions and cancer in the highest area of cervical cancer mortality, Longnan, China

Human papillomavirus genotypes associated with cervical precancerous lesions and cancer in the highest area of cervical cancer mortality, Longnan, China

Prevalence characteristics of single and multiple HPV infections in women with cervical cancer and precancerous lesions in Beijing, China

Production of Polyclonal Antibody to the HPV58 E7 Protein and Its Detection in Cervical Cancer

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