Prevalence characteristics of single and multiple HPV infections in women with cervical cancer and precancerous lesions in Beijing, China

Li, Mingxia; Du, Xinxin; Lu, Menghan; Zhang, Weiyi; Sun, Zhihui; Lian, Li; Ye, Mingxia; Fan, Wensheng; Jiang, Sufei; Liu, Aijun; Wang, Min; Meng, Yuanguang; Li, Yali

doi:10.1002/jmv.25331

Cited by 59 publications

(74 citation statements)

References 51 publications

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“…Coinfection with multiple types of HPV was more commonly observed in women with precancerous lesions (between 15% and 41%) than in normal cytology (between 1% and 3%) and invasive cervical cancer (between 6% and 8%) . Regarding the ADC, the prevalence of 8.4% for HPV coinfections was found in our study, consistent with that of 7.0% in a worldwide meta‐analysis of 760 ADC cases, and 8.5% in a study of 1408 ADC cases worldwide .…”

Section: Discussionsupporting

confidence: 90%

Clustering patterns of type‐type combination in multiple genotypes infections of human papillomavirus in cervical adenocarcinoma

et al. 2019

Journal of Medical Virology

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Coinfections with multiple types of human papillomavirus (HPV) occur in cervical adenocarcinoma (ADC). However, it remains unclear the clustering patterns of multiple types in HPV coinfections and relevant factors in ADC. A total of 718 paraffin-embedded ADC specimens were collected in China and tested for HPV genotypes using SPF10-INNO-LIPA. The prevalence of HPV coinfections and clustering patterns by geographical regions, histological subtypes and ages were assessed. Type-specific attribution of HPV to ADC adjusted by HPV coinfections were calculated. The prevalence of HPV coinfections was found to be 8.4% in ADC cases with slight variation by geographic regions between 2.2% and 12.5%. The 88.3% of all HPV multiple infections in ADC were two types of HPV coinfections with predominant combination of HPV 16 and HPV 18. The attribution to ADC was 88.0% for HPV 16/18 targeted by bivalent and quadrivalent vaccine and 96.8% for HPV 16/ 18/31/33/45/52/58 targeted by nonavalent vaccine. Clustering patterns of multiple types were related with histological categories and age at diagnosis. In conclusion, HPV coinfections are uncommonly prevalent in ADC cases with slight variation by geographic regions and distinct clustering patterns of multiple types by histological subtypes and ages at diagnosis. The high attribution of carcinogenic HPV types to ADC predicts potential protection of HPV vaccine against ADC. K E Y W O R D S cervical adenocarcinoma, clustering patterns, human papillomavirus, multiple genotypes 1 | INTRODUCTION Cervical adenocarcinoma (ADC), a histological subtype of cervical cancer, composes approximately 10% to 20% of all cervical cancer cases. 1,2 In contrast with the declined incidence of squamous cell cancer in an era of ever-increasing coverage of cervical cancer screening and human papillomavirus (HPV) vaccine, ADC has become more common in the past decades, even in countries with organized screening. 3-6 The estimated global incidence of ADC was 1.6 cases per 100 000 women, and approximately 56 805 new cases developed worldwide in 2015. 2 Persistent infection with high-risk types of HPV has been established as an essential cause of cervical cancer. 7,8 Over 200 types of HPV out of more than 350 different papillomaviruses Li Dong and Tingyuan Li contributed equally to this work.

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Section: Discussionsupporting

confidence: 90%

Clustering patterns of type‐type combination in multiple genotypes infections of human papillomavirus in cervical adenocarcinoma

et al. 2019

Journal of Medical Virology

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“…It has been noted that genotypes 16 and 18 are present in >70% of SCC cases ( 3 ). A number of studies have reported that MI with HR-HPV can increase the risk of cervical intraepithelial neoplasia (CIN) progressing to SCC ( 33 – 35 ). However, other reports that HPV16, in itself, can increase the risk of developing a HSIL should also be considered ( 36 ).…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, liquid-based cytology smears were submitted to permeabilization for 30 min at 120˚C, followed by enzymatic digestion for 5 sec with K proteinase (1:1,000). The samples were then added to 1 µl test reagent (Dako GentPoint TM HPV DNA Probe Cocktail, Biotinylated) for 13 HR-HPV genotypes (16,18,31,33,35,39,45,51,52,56,58,59 and 68; Agilent Technologies, Inc.). The slides were subjected to DNA denaturation for 10 min at 95˚C and hybridization for 20 h at 37˚C on a Dako Hybridizer (Agilent Technologies, Inc.).…”

Section: Methodsmentioning

confidence: 99%

An increase of microRNA‑16‑1 is associated with the high proliferation of squamous intraepithelial lesions in the presence of the integrated state of HR‑HPV in liquid cytology samples

et al. 2020

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Studies of cervical cancer (CC) have reported that microRNA-16-1 (miR-16-1), which is an oncomiR, is increased in the tissues and cell lines of CC. The aim of the present study was to investigate the association of miRNA-16-1 expression level with squamous cell carcinoma (SCC), the presence of squamous intraepithelial lesions (SIL) and the integration of high-risk human papillomavirus (HR-HPV) DNA. The current study analyzed 80 samples obtained from women by liquid-based cytology, which revealed that 20 were negative for SIL (NSIL) and without HPV, 20 were low-grade SIL (LSIL), 20 were high-grade SIL (HSIL), and 20 were diagnosed as SCC with HR-HPV. The genotyping of the viral DNA was conducted via an INNO-LiPA-HPV array, the expression of miR-16-1 was determined by reverse transcription-quantitative PCR, and the physical state of the HR-HPV was ascertained by in situ hybridization with amplification with tyramide. A total of eight HR-HPV genotypes were distinguished; the most frequent of these being HPV16, followed by multiple infection with HR-HPV (including HPV16). The mixed state of the HR-HPV was observed in 60 and 65% of LSIL and HSIL cases, respectively, while an integrated HR-HPV state was identified in 90% of cases with SCC. The expression level of miR-16-1 increased according to the grade of SIL, and cases with HSIL exhibited a significantly higher miR-16-1 expression level compared with women with NSIL (P<0.001; Table II). It can therefore be determined that the expression of miR-16-1 effects cellular proliferation, due to the viral integration of various HR-HPV genotypes in unique infection or in multiple infection. Thus, the overexpression of miR-16-1 could be monitored in women with LSIL, in order to discard a major lesion.

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“…An essential point in the study of the significance of HR-HPV coinfection is whether multiple infections are clinically relevant, i.e., if coinfection is associated with an increased or reduced risk of CIN or cancer or with an increased risk of progression or recurrence of CIN. Numerous studies have associated overall multiple HR-HPV infections with an increased prevalence and severity of CIN, especially among young women with multiple sexual partners [ 22 ] or HIV infected subjects [ 23 ]. Multiple HR-HPV infections have also been associated with larger colposcopic lesions [ 10 ] and increased risk of progression among subjects with ASCUS and LSIL on cytology [ 24 ], and high risk of treatment failure among women with invasive cervical cancer [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Clinical Significance of the Interaction between Human Papillomavirus (HPV) Type 16 and Other High-Risk Human Papillomaviruses in Women with Cervical Intraepithelial Neoplasia (CIN) and Invasive Cervical Cancer

Spinillo

Dominoni

Boschi

et al. 2020

Journal of Oncology

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The aim is to evaluate the clinical consequences of coinfection between HPV 16 and other high-risk HPVs among women with a histological diagnosis of CIN or invasive cervical cancer. A total of 2985 women, with a diagnosis of either CIN or cancer (<IB) on cervical or cone biopsy, were included. HPV genotypes were identified using the INNO-LiPA HPV genotyping assay, version EXTRA, on cervical scraping, before the colposcopic evaluation and the colposcopic biopsies or conization. In the overall population, HPV16 interacted positively with HPV18 (RR = 2, 95% CI 1.5–2.6) and negatively with HPV33, 51, 52, and 66, in log-linear analysis. There was an excess of CIN3 diagnoses among subjects coinfected with HPV16 and HPV18 or HPV52, although the absolute number of cases was relatively small. In a logistic model, the odds ratio of CIN3+ associated with coinfection of HPV16 and HPV18 (OR = 3.8, 95% CI 2.5–5.7, p = 0.004 compared to single HPV16) or HPV52 (OR = 3.6, 95% CI 2.6–5.1, p = 0.009 compared to single HPV) was higher than that associated with single HPV 16 infections. Finally, multiple infections had no effect on residual disease and did not influence the recurrence of high-grade CIN during a median follow-up of 25 months (IR 17–41). HPV16 interacted positively with HPV18 and negatively with HPV33, 51, 52, and 66 supporting the notion that HPV16 interacts mostly negatively with other HR-HPVs in CIN lesions. Among specimens coinfected with HPV16 and 18 or 52, there was an excess of CIN3+ although the impact on the prevalence of severe cervical lesions was limited.

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Prevalence characteristics of single and multiple HPV infections in women with cervical cancer and precancerous lesions in Beijing, China

Cited by 59 publications

References 51 publications

Clustering patterns of type‐type combination in multiple genotypes infections of human papillomavirus in cervical adenocarcinoma

Clustering patterns of type‐type combination in multiple genotypes infections of human papillomavirus in cervical adenocarcinoma

An increase of microRNA‑16‑1 is associated with the high proliferation of squamous intraepithelial lesions in the presence of the integrated state of HR‑HPV in liquid cytology samples

Clinical Significance of the Interaction between Human Papillomavirus (HPV) Type 16 and Other High-Risk Human Papillomaviruses in Women with Cervical Intraepithelial Neoplasia (CIN) and Invasive Cervical Cancer

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