Prevalence, Antibiotic Resistance, and Molecular Characterizatio of Salmonella Serovars in Retail Meat Products

Abstract

The prevalence of Salmonella was determined in chicken meat (n = 26), beef (n = 49), and pork (n = 56) collected from wholesale markets, retail stores, and traditional markets in Seoul, South Korea, in 2009. Antibiotic resistance was assessed, and the molecular subtypes of Salmonella isolates were ascertained using an automated repetitive sequence-based PCR (rep-PCR) system (DiversiLab). A total of 18 Salmonella strains were isolated from 17 of 131 samples: 16 strains from each of 16 samples and 2 strains from the same pork sample. The prevalence of Salmonella from the retail meats was 2.0% in beef, 8.9% in pork, and 42.3% in chicken meat. Among 10 different serotypes, Salmonella enterica Panama was recovered from a beef sample, and Salmonella London and Salmonella Montevideo were the predominant serotypes from pork and chicken meat, respectively. The highest antibiotic resistance observed was to erythromycin (100%) followed by streptomycin (22.2%) and tetracycline and chloramphenicol (16.7%). Of the 18 isolates, 5 (27.8%) were resistant to two or more antibiotics, and 1 isolate from chicken meat was resistant to eight antibiotics, including cephalosporins. Differentiation between all of the Salmonella isolates except between Salmonella Montevideo and Salmonella London was successfully performed with the automated rep-PCR system, indicating that it can be added to the toolbox for source tracking of foodborne pathogens associated with outbreaks.

Isolation, molecular identification and antibiogram profiles of Escherichia coli and Salmonella spp. from diarrhoeic cattle reared in selected areas of Bangladesh

“…In this study, 8 different antibiotics available in the market were used to study antibiogram profiles of the E. coli and Salmonella spp. isolated from diarrhoeic cattle by disc diffusion method and the results were supported by Akond et al, 2009;Wouafo et al, 2010;Hyeon et al, 2011;De et al, 2012;Jeyasanta et al, 2012;Jahan et al, 2013. Due to financial crisis and limited time following further studies like collection of more samples covering many areas and farms of Bangladesh to get more precise information, molecular detection of shiga toxin producing E. coli, molecular characterization of the isolated bacterial species by pulsed field gel electrophoresis (PFGE), 16S rRNA gene sequencing of the isolated E. coli and Salmonella spp., serotyping, multiplex PCR of the isolated Salmonella spp., pathogenicity study would not be possible.…”

Salmonella: A review on pathogenesis, epidemiology and antibiotic resistance

“…The emergence of Salmonella with antimicrobial resistance is mainly promoted by the use of antibiotics in animal feed to promote the growth of food animals, and in veterinary medicine to treat bacterial infections in those animals (Hyeon et al 2011). This poses a high risk of zoonotic disease with the transmission of MDR Salmonella strains from animals to humans via the ingestion of food or water contaminated with the animals' faeces, direct contact or the consumption of infected food animals (Holmberg et al 1984).…”

Isolation, identification, and antibiogram studies of Salmonella species and Escherichia coli from boiler meat in some selected areas of Bangladesh

“…[15][16][17] Isolates of E. coli observed in EMB agar revealed smooth, circular, greenish black color colonies with metallic sheen and pink color colonies on McConkey agar. [18][19][20][21] In Gram's staining, the morphology of the isolated bacteria exhibited Gram-negative, short rod arranged in single or paired. 22,23 The Salmonella revealed smooth, circular, black centered colonies on XLD agar.…”