Prevalence and Phylogenic Study of Human T-lymphotropic Virus 1 in Patients With Thalassemia in the Northeast of Iran

Abedi, Farshid; Mozhgani, Sayed‐Hamidreza; Rahimzadegan, Marzieh; Gudarzi, Hoda; Valizadeh, Narges; Rezaee, Seyed Abdolrahim

doi:10.2217/fvl-2016-0107

Cited by 7 publications

(2 citation statements)

References 25 publications

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“…To the best of the best of our knowledge, no previous phylogenetic studies were conducted in Tehran and Urmia. Previous studies in Iran, such as Sabzevar, Torbat‐e Heydarieh, and Mashhad revealed that HTLV‐1 genotype belongs to the subtype a and the related subgroup was only reported in a recent study in Mashhad . Studied populations in these surveys were varied; studies about people born in Mashhad show that the HTLV‐1 genotype belongs to the cosmopolitan clade .…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of HTLV‐1 in Iranian blood donors, HIV‐1 positive patients and patients with beta thalassemia

Pirayeshfard

Sharifi

Amini-Kafiabad

et al. 2018

Journal of Medical Virology

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Human T-cell lymphoma virus (HTLV) has been associated with various disease types. Since the discovery of the virus in 1980, seven subtypes of the virus have been identified. HTLV is widespread and endemic in some regions, such as Japan, Africa, South America, and northeast Iran. This study aimed to identify HTLV-1 genotype and also to analyze the nucleotide sequence of the LTR region in three groups, including blood donors, HIV-1+ patients, and β-thalassemia patients. In this cross-sectional study, 2200 samples were collected from blood donors in Tehran (2000 samples), HIV-1+ patients (100 samples) and β-thalassemia patients (100 samples). All samples were screened for anti-HTLV-I&II antibodies by ELISA. Then, genomic DNA was extracted from repeatedly positive samples, and nested PCR was performed for both the TAX and LTR regions. Purified PCR products were sequenced and analyzed, and finally, a phylogenetic tree was constructed using Mega7 software. The prevalence of the anti-HTLV-I&II antibody among blood donors and HIV-1+ patients was 1.7% (34/2000) and 12% (12/100), respectively. The PCR results confirmed that 0.05% (1/2000) of blood donors, 5% (5/100) of HIV-1+ patients, and 8% (8/100) of β-thalassemia patients were HTLV-I positive. All sequences were matched to HTLV-1 subtype a, subgroup A. Our phylogenetic analysis revealed that all sequenced samples belong to the endemic clusters of Iran. HTLV-1 genotypes in all samples were similar in three groups and were derived from the strains, which had been previously reported from Iran (AF00300/Mashhad and KT190712.1/Sabzevar).

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Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of HTLV‐1 in Iranian blood donors, HIV‐1 positive patients and patients with beta thalassemia

Pirayeshfard

Sharifi

Amini-Kafiabad

et al. 2018

Journal of Medical Virology

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“…It is difficult to detect the provirus due to the rare proviral DNA expression in the infected host for some weeks, while the oncogenicity may be at high risk [3,4]. Human Immunodeficiency Virus (HIV) and Human T-cell lymphotropic virus (HTLV) are two kinds of human retroviruses that cause diseases with a high mortality potential [3,5,6]. Despite the joint transmission routes between HIV-1 and HTLV-1, they lead to remarkable different diseases.…”

Section: Introductionmentioning

confidence: 99%

Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection

Mozhgani

Kermani

Norouzi

et al. 2020

Heliyon

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Early detection of retroviruses including human T-cell lymphotropic virus and human immunodeficiency virus in the human body is indispensable to prevent retroviral infection propagation and improve clinical treatment. Until now, diverse techniques have been employed for the early detection of viruses. Traditional methods are timeconsuming, resource-intensive, and laborious performing. Therefore, designing and constructing a selective and sensitive diagnosis system to detect serious diseases is highly demanded. Genetic detection with high sensitivity has striking significance for the early detection and remedy of disparate pathogenic diseases. The nucleic acid biosensors are based on the identification of specific DNA sequences in biological samples. Nanotechnology has an important impact on the development of sensitive biosensors. Different kinds of nanomaterials include nanoparticles, nanoclusters, quantum dots, carbon nanotubes, nanocomposites, etc., with different properties have been used to improve the performance of biosensors. Recently, DNA nanobiosensors are developed to provide simple, fast, selective, low-cost, and sensitive detection of infectious diseases. In this paper, the research progresses of nano genosensors for the detection of HIV-1 and HTLV-1 viruses, based on electrochemical, optical, and photoelectrochemical platforms are overviewed.

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Current approaches for detection of human T‐lymphotropic virus Type 1: A systematic review

Fani

Rezayi

Meshkat

et al. 2019

Journal Cellular Physiology

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Background Human T‐lymphotropic virus Type 1 (HTLV‐1) is a retrovirus that is endemic in some regions of the world. It is known to cause several diseases like adult T‐cell leukemia (ATL) and HTLV‐1‐associated myelopathy/tropical spastic paraparesis (HAM/TSP). Serology and molecular methods have been used to detect this virus. Of these, enzyme‐linked immunosorbent assay (ELISA) is used as a primary screening method and this is usually followed by western blotting (WB) and polymerase chain reaction (PCR) methods as confirmatory tests. We conducted a systematic review of the different techniques used in the diagnosis of HTLV‐1 infection. Materials and Methods Our search was limited to original papers in the English language from 2010 to 2018 using several databases including Pubmed, Scopus, Google Scholar, Iranmedex, and Scientific Information Database. A manual search of references provided in the included papers was also performed. Results Of 101 electronically searched citations, 43 met the inclusion criteria. ELISA is commonly used for qualitative and screening detection, and WB and PCR techniques are used to confirm infection. Conclusion Among all the reported methods for detection of HTLV‐1, only serological and molecular tests are used as the most common technical assays for HTLV‐1. The ELISA assay, without a confirmatory test, has several limitations and affect the accuracy of the results. Owing to the prevalence of HTLV‐1 and limitations of the current detection methods, further evaluation of the accuracy of these methods is needed. There are new opportunities for applying novel technological advances in microfluidics, biosensors, and lab‐on‐a‐chip systems to perform HTLV‐1 diagnostics.

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Prevalence and Phylogenic Study of Human T-lymphotropic Virus 1 in Patients With Thalassemia in the Northeast of Iran

Cited by 7 publications

References 25 publications

Phylogenetic analysis of HTLV‐1 in Iranian blood donors, HIV‐1 positive patients and patients with beta thalassemia

Phylogenetic analysis of HTLV‐1 in Iranian blood donors, HIV‐1 positive patients and patients with beta thalassemia

Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection

Current approaches for detection of human T‐lymphotropic virus Type 1: A systematic review

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