Prevalence and Molecular Epidemiological Characterization of Antimicrobial-Resistant Escherichia coli Isolates from Japanese Black Beef Cattle

Yamamoto, Shiori; Iwabuchi, Eriko; Hasegawa, Megumi; Esaki, Hidetake; Muramatsu, Masatake; Hirayama, Norio; Hirai, Katsuya

doi:10.4315/0362-028x.jfp-12-273

Cited by 23 publications

(23 citation statements)

References 29 publications

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“…However, the amount of veterinary antimicrobial agents (therapeutic and feed additive) sold per food-producing animal weight (pig, broiler, and cattle) in Japan increased from 132 mg kg −1 to 153 mg kg −1 from 2005 to 2010 (25). The use of antibiotics in food animals is increasing, as is the frequency of AMR strains on livestock farms (25, 43, http://www.maff.go.jp/nval/tyosa_kenkyu/taiseiki/index.html). …”

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“…In 2009, we obtained 3,147 E. coli isolates from the feces of beef cattle on 14 farms in three Japanese regions (Hokkaido, Chubu, and Kyushu) (43) and assessed these isolates for antibiotic resistance. We found that 44.4% (1,347 isolates) of the isolates were AMR, which represented a higher frequency than previously reported (13, 24, 40).…”

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confidence: 99%

“…These isolates were resistant to nine or more antimicrobial agents and were selected from the 3,147 isolates obtained in our previous study (43). To understand the genetic backgrounds and phylogenetic relationships of AMR in these isolates, we analyzed their chromosome phylotype, AMR phenotype, AMR genotype, and plasmid incompatibility type.…”

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Characterization of Multi-antibiotic-resistant <i>Escherichia coli</i> Isolated from Beef Cattle in Japan

et al. 2014

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The emergence of multiple-antibiotic-resistance bacteria is increasing, which is a particular concern on livestock farms. We previously isolated 1,347 antimicrobial-resistant (AMR) Escherichia coli strains from the feces of beef cattle on 14 Japanese farms. In the present study, the genetic backgrounds and phylogenetic relationships of 45 AMR isolates were characterized by the chromosome phylotype, AMR phenotype, AMR genotype, and plasmid type. These isolates were classified into five chromosome phylotypes, which were closely linked to the farms from which they were isolated, suggesting that each farm had its own E. coli phylotype. AMR phenotype and plasmid type analyses yielded 8 and 14 types, all of which were associated with the chromosomal phylotype and, thus, to the original farms. AMR genotype analysis revealed more variety, with 16 types, indicating both inter- and intra-farm diversity. Different phylotype isolates from the same farm shared highly similar plasmid types, which indicated that plasmids with AMR genes could be transferred between phylotypes, thereby generating multi-antibiotic-resistant microorganisms. This ecological study demonstrated that the chromosome phylotype was strongly correlated with the farm from which they were isolated, while the AMR phenotype, genotype, and plasmid type were generally correlated with the chromosome phylotype and farm source.

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Characterization of Multi-antibiotic-resistant <i>Escherichia coli</i> Isolated from Beef Cattle in Japan

et al. 2014

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“…The MLVA was not used to test movement within farms; however, we can assume, based on these results, that the bacterial flora surrounding the calves may play an essential role in the presence of bacteria in their gastrointestinal (GI) tract, which was also observed in a recent study by Liu et al (13). A high prevalence of QREC in feed and water troughs may ease the fecal-oral circulation of QREC, which was also suggested by Yamamoto et al (14) to be important for the on-farm dissemination of multidrug-resistant E. coli. The higher that the proportion of QREC isolates in the calf feed and water troughs is, the more likely it is that QREC establishes itself in the calf gut and the more likely it is that the calf recontaminates the pen environment.…”

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confidence: 76%

Occurrence and Spread of Quinolone-Resistant Escherichia coli on Dairy Farms

Duse

Waller

Emanuelson

et al. 2016

Appl Environ Microbiol

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Quinolone-resistant Escherichia coli (QREC) is common in feces from young calves, but the prevalence and genetic diversity of QREC in groups of cattle of other ages and the farm environment are unknown. The aims of the study were to obtain knowledge about the occurrence of QREC on dairy farms, the genetic diversity of QREC within and between farms, and how these relate to the number of purchased animals and geographic distances between farms. We analyzed the within-sample prevalence of QREC in individual fecal samples from preweaned dairy calves and postpartum cows and in environmental samples from 23 Swedish dairy farms. The genetic diversity of the QREC isolates on 10 of these farms was assessed. In general, QREC was more prevalent in the dairy farm environment and in postpartum cows if QREC was commonly found in calves than if QREC was rare in calves. In particular, we found more QREC organisms in feed and water troughs and in environments that may come into contact with young calves. Thus, the results suggest that QREC circulates between cattle and the farm environment and that calves are important for the maintenance of QREC. Some genotypes of QREC were widespread both within and between farms, indicating that QREC has spread within the farms and likely also between farms, possibly through purchased animals. Farms that had purchased many animals over the years had greater within-farm diversity than farms with more closed animal populations. Finally, animals on more closely located farms were more likely to share the same genotype than animals on farms located far apart. IMPORTANCEThis study investigates the occurrence of a specific type of antimicrobial-resistant bacterium on dairy farms. It contributes to increased knowledge about the occurrence and spread of these bacteria, and the results pave the way for actions or further studies that could help mitigate the spread of these bacteria on dairy farms and in the community as a whole.

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“…19 Horizontal transferring of resistant bacteria and genes to environment, foods, and other hosts is completely probable. 20 Therefore, tracing AR in commensal microorganisms can help us to adopt strategies for controlling resistance.…”

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Antimicrobial Resistance Characteristics and Phylogenetic Groups of Escherichia coli Isolated From Diarrheic Calves in Southeast of Iran

Naderi¹,

Ghanbarpour²,

Sami³

2016

Int J Enteric Pathog

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Prevalence and Molecular Epidemiological Characterization of Antimicrobial-Resistant Escherichia coli Isolates from Japanese Black Beef Cattle

Cited by 23 publications

References 29 publications

Characterization of Multi-antibiotic-resistant <i>Escherichia coli</i> Isolated from Beef Cattle in Japan

Characterization of Multi-antibiotic-resistant <i>Escherichia coli</i> Isolated from Beef Cattle in Japan

Occurrence and Spread of Quinolone-Resistant Escherichia coli on Dairy Farms

Antimicrobial Resistance Characteristics and Phylogenetic Groups of Escherichia coli Isolated From Diarrheic Calves in Southeast of Iran

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