Antimicrobial Resistance Characteristics and Phylogenetic Groups of Escherichia coli Isolated From Diarrheic Calves in Southeast of Iran

Background: Antibiotic resistance (AR) is an important challenge in prevention, treatment and control of infectious diseases and is a public health threat for human. Escherichia coli strains are the major causes of urinary tract infections (UTIs). Objective: This research aimed to determine prevalence of resistance to quinolone and fluoroquinolone antibiotics and screen qnr genes among E. coli isolates from UTIs. Materials and Methods: A total of 105 E. coli isolates were obtained from UTI cases in Bojnord city (northeast of Iran) and confirmed by biochemical tests. All strains were studied to determine their resistance to 3 antibiotics including ciprofloxacin, nalidixic acid, and levofloxacin via disk diffusion method. Moreover, the frequency of qnrA, qnrB and qnrS genes and phylogroups was studied by conventional Polymerase chain reaction (PCR). Results: In this study, prevalence of phenotypic AR to ciprofloxacin, nalidixic acid and levofloxacin was 47.6%, 44.8% and 38.1%, respectively. Three isolates were positive for qnrS and 1 isolate was positive for qnrA. Seven phylogenetic groups were also identified as follows: 18% A0, 6.7% A1, 7.6% B1, 1.9% B22, 23.8% B23, 15.3% D1 and 26.7% D2. Conclusion: Prevalence of qnr genes was very low; thus, other types of qnr and plasmid-mediated quinolone resistance genes were probably responsible for the resistance. Phenotypic AR to the 3 antibiotics was found in approximately half of E. coli strains. It is strongly recommended that antibiogram tests should be done before prescribing the ciprofloxacin, nalidixic acid and levofloxacin for UTIs.

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“…13 As positive control for qnr genes, we used qnr-positive clinical strains which Naderi et al previously found. 14…”

Section: Antibiogram For Identification Of Escherichia Coli Isolates mentioning

confidence: 99%

Prevalence of Resistance to Quinolone and Fluoroquinolone Antibiotics and Screening of qnr Genes Among Escherichia coli Isolates From Urinary Tract Infection

Amiri

Jajarmi

Ghanbarpour

2017

Int J Enteric Pathog

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Antibiotic resistance profiling and phylotyping of human-diarrheagenic Escherichia coli pathotypes detected from diarrheic and non-diarrheic calves in Iran

Naderi,

Ghanbarpour,

Jajarmi

et al. 2024

Mol Biol Rep

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Determination of Inherent Antibiotic Drug Resistance and Pathogenic Potential of Different E. coli Phylotypes from Diarrheic Calves in Arid and Semiarid Regions of India

Kumar

Choudhary

Kumar

et al. 2022

Preprint

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India has a large population of dairy animals, and calf diarrhea is a major concern in the dairy sector. Escherichia coli have varying degrees of virulence; however, this study aimed to understand the association of different phylotypes with calf diarrhea and elucidate their inherent virulence potential as well as their antimicrobial drug resistance potential. In the current study, the phylogenetic analysis showed that the majority of the isolates (19/35; 54.28%) belonged to phylotype B1, followed by A (9/35; 20%), E (4/35; 11.42%), C (2/35; 5.71%), and F (2/35; 5.71%).On the basis of virulence genes, isolates were classified into ETEC (25.71%), STEC (48.57%) and non-ETEC/STEC (25.71%). PhylotypesB1 and F predominated in virulence potential, followed by phylotypesC and E. Both isolates of phylotype F were found to be STEC pathotypes. PhylotypesF and B1 were found to carry antimicrobial resistance genes against all four classes of antimicrobials, while phylotype E carried antimicrobial drug resistance genes for three classes, and phylotypes C and A showed the least number of resistance genes. In conclusion, isolates belonging to phylotypes B1 and F were of high pathogenic potential due to the carriage of a higher number of virulence and antibiotic resistance genes.

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Antimicrobial Resistance Characteristics and Phylogenetic Groups of Escherichia coli Isolated From Diarrheic Calves in Southeast of Iran

Cited by 4 publications

References 16 publications

Prevalence of Resistance to Quinolone and Fluoroquinolone Antibiotics and Screening of qnr Genes Among Escherichia coli Isolates From Urinary Tract Infection

Prevalence of Resistance to Quinolone and Fluoroquinolone Antibiotics and Screening of qnr Genes Among Escherichia coli Isolates From Urinary Tract Infection

Antibiotic resistance profiling and phylotyping of human-diarrheagenic Escherichia coli pathotypes detected from diarrheic and non-diarrheic calves in Iran

Determination of Inherent Antibiotic Drug Resistance and Pathogenic Potential of Different E. coli Phylotypes from Diarrheic Calves in Arid and Semiarid Regions of India

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