Prevalence and characteristics of Escherichia coli O157 from major food animals in Korea

Jo, Mi Yeong; Kim, Ji Hyun; Lim, Jae Hyang; Kang, Mi Young; Koh, Hong Bum; Park, Taesung; Yoon, Do Young; Chae, Joon; Eo, Seong Kug; Lee, John Hwa

doi:10.1016/j.ijfoodmicro.2004.01.016

Cited by 60 publications

(46 citation statements)

References 44 publications

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“…Among the major infectious agents, Escherichia coli O157:H7 has frequently been associated with foodborne illness. Particularly, over the past decade, E. coli O157:H7 has been reported increasingly from all parts of the world and in the worst case, it is "one of the most serious" foodborne pathogens leading to severe illnesses and high mortality rates in humans (Blanco et al 2003;Jo et al 2004). This consideration is in fact due to the small infectious dose of the organism because fewer than 40 cells of E. coli O157:H7 can cause illness in some people (Strachan et al 2005).…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Occurrence and antimicrobial susceptibility profile of Escherichia coli O157:H7 from food of animal origin in Bishoftu town, Central Ethiopia

et al. 2018

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Background: Escherichia coli O157:H7 (E. coli O157:H7) have frequently been associated with food borne illness and are considered as most serious of known food borne pathogens leading to severe illnesses and high mortality rates in humans. Most of outbreaks were traced to raw meat and raw milk consumption, as well as to dairy products such as yogurt and cheese derived from raw milk. Results: Out of 200 samples examined, 40 (20%) and 7 (3.5%) of the samples were positive to E. coli and E. coli O157: H7 respectively. The highest isolation of E. coli was from cheese (40%), followed by raw milk (32%), yogurt (25.71%), beef (13.84%), and pasteurized milk (0%). Among E. coli O157:H7 isolates, the highest isolation was from raw milk (12%) followed by cheese (5.71%) and meat (3.07%). However, no E. coli O157:H7 was isolated from pasteurized milk and yogurt. Antibiotic susceptibility profile showed that E. coli was resistant for vancomycin (89.74%), ampicillin (76.92%) and streptomycin (69.23%). The analysis showed that, 92.5% of isolates showed multidrug resistance comprising 2-4 antimicrobials. Conclusion: The occurrence of E. coli O157:H7 and its multiple antibiotic resistant profiles shows a risk for public health and food safety as well as animal production. These findings stress the need for an integrated control of E. coli O157:H7 from farm production to consumption of food of animal origin.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Occurrence and antimicrobial susceptibility profile of Escherichia coli O157:H7 from food of animal origin in Bishoftu town, Central Ethiopia

et al. 2018

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“…A lower result was reported in a previous study by Akbar et al (8), who isolated E. coli O157 from 2% of poultry meat samples in Thailand. However, in studies conducted by Jo et al (14) in Korea and Abay et al (15) in Turkey, none of the E. coli O157 isolates were positive in chicken meat samples. Similar results (4% and 10.3%) were reported by Abdul-Raouf et al (16) from Egypt and Chinen et al (17) from Argentina, respectively.…”

Section: Discussionmentioning

confidence: 79%

“…In another study, conducted by Dipineto et al (25), eaeA and stx2 genes were detected in all 26 STEC isolated from 720 cloacal samples of layer hens, while Momtaz and Jamshidi (26) found stx1, eaeA, and ehlyA virulence genes in 31 (21.23%) EHEC isolates in Iran. However, according to some other researchers (14,27), the presence of neither E. coli O157 isolates nor their virulence genes was found.…”

Section: Discussionmentioning

confidence: 83%

Antimicrobial resistance profiles and virulence factors of Escherichia coliO157 collected from a poultry processing plant*

Hızlısoy¹,

Al²,

Onmaz³

et al. 2017

Turk J Vet Anim Sci

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IntroductionEscherichia coli O157 and other enterohemorrhagic E. coli (EHEC) are a worldwide threat to public health (1). Mostly in developed countries, E. coli O157 has been recognized as a cause of serious clinical symptoms such as hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS) characterized by thrombocytopenia, hemolytic anemia, and nephropathy (2). Cattle are the primary reservoir and asymptomatic carriers of E. coli O157. The bacteria were spread via cattle faces (3). Most outbreaks of EHEC infections have been associated with the consumption of uncooked meat and meat products, unpasteurized dairy products, and feces-contaminated vegetables and water (2-4).E. coli O157, a member of EHEC, has important virulence factors such as shiga toxins (stx1 and stx2), enterohemolysin (hlyA), and intimin (eaeA). Shiga toxinproducing E. coli (STEC) can cause HC and HUS (5,6) due to the inhibiting protein synthesis, eukaryotic cell injury, and death (7). Intimin, encoded by the eaeA gene, is able to adhere to intestinal mucosa and cause intestinal lesions (5,7). In addition, enterohemolysin, encoded by hlyA, leads to lysis of the erythrocytes, which may contribute to the iron intake of the bacterium to live in the intestine (4).The frequency of antimicrobial resistance in foodborne pathogens such as E. coli, Salmonella spp., and Campylobacter spp. has recently increased. The misuse of antimicrobial agents for farming and therapeutic purpose in animals and humans is the main cause of transmission of antibiotic-resistant strains, which are very difficult to treat with commonly used antibiotics, to humans via the food supply (8).The aim of this study was to investigate the potential risk of public health by determining the prevalence of E. coli O157:H7 and identifying the virulence genes from The aims of this study were to investigate the prevalence of E. coli O157 and to identify virulence genes and also to determine the antibiotic susceptibility of the isolates in different poultry-originated materials.

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“…Cattle are an important asymptomatic reservoir host of STEC O157, which may be transmitted to humans by ingestion of contaminated meat, produce, or water or by direct contact with cattle carrying the pathogen (4)(5)(6). The seasonal variation in STEC O157 fecal shedding by cattle, specifically the higher summertime prevalence, occurs in diverse regions, including Canada, England, Italy, South Korea, the Netherlands, Turkey, and the United States (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20). Similar summertime peak shedding is also reported in sheep (11,15,21).…”

mentioning

confidence: 99%

Standardized Escherichia coli O157:H7 Exposure Studies in Cattle Provide Evidence that Bovine Factors Do Not Drive Increased Summertime Colonization

Sheng

Shringi

Baker

et al. 2016

Appl Environ Microbiol

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The increased summertime prevalence of cattle carriage of enterohemorrhagic Shiga toxin-producing Escherichia coli O157:H7 (STEC O157) is associated with the increased summertime incidence of human infection. The mechanism driving the seasonality of STEC O157 carriage among cattle is unknown. We conducted experimental challenge trials to distinguish whether factors extrinsic or intrinsic to cattle underlie the seasonality of STEC O157 colonization. Holstein steers (n ‫؍‬ 20) exposed to ambient environmental conditions were challenged with a standardized pool of STEC O157 strains four times at 6-month intervals. The densities and durations of rectoanal junction mucosa (RAJ) colonization with STEC O157 were compared by season (winter versus summer), dose (10 9 CFU versus 10 7 CFU), and route of challenge (oral versus rectal). Following summer challenges, the RAJ STEC O157 colonization density was significantly lower (P ‫؍‬ 0.016) and the duration was shorter (P ‫؍‬ 0.052) than for winter challenges, a seasonal pattern opposite to that observed naturally. Colonization was unaffected by the challenge route, indicating that passage through the gastrointestinal microbiome did not significantly affect the infectious dose to the RAJ. A 2-log reduction of the challenge doses in the second-year trials was accompanied by similarly reduced RAJ colonization in both seasons (P < 0.001). These results refute the hypothesis that cattle are predisposed to STEC O157 colonization during the summer months, either due to intrinsic factors or indirectly due to gastrointestinal tract microbiome effects. Instead, the data support the hypothesis that the increased summertime STEC O157 colonization results from increased seasonal oral exposure to this pathogen. Shiga toxin-producing Escherichia coli serotype O157:H7 (STEC O157) is an important zoonotic pathogen estimated to cause Ͼ70,000 cases of human infection annually in the United States (1, 2). Human disease is characterized by mild to severe, frequently hemorrhagic diarrhea, and the development of a severe sequela, the hemolytic-uremic syndrome, in approximately 5 to 10% of patients (3). Cattle are an important asymptomatic reservoir host of STEC O157, which may be transmitted to humans by ingestion of contaminated meat, produce, or water or by direct contact with cattle carrying the pathogen (4-6). The seasonal variation in STEC O157 fecal shedding by cattle, specifically the higher summertime prevalence, occurs in diverse regions, including Canada, England, Italy, South Korea, the Netherlands, Turkey, and the United States (7-20). Similar summertime peak shedding is also reported in sheep (11,15,21). Contrary patterns have been occasionally reported, including a higher wintertime prevalence (22, 23) or no seasonal variation (24-26); however, these are unusual and sometimes are confounded by other factors such as a change to indoor housing (22).The seasonal summertime shedding of STEC O157 in cattle parallels both seasonal increases in carcass contamination at abattoirs and the in...

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Prevalence and characteristics of Escherichia coli O157 from major food animals in Korea

Cited by 60 publications

References 44 publications

RETRACTED ARTICLE: Occurrence and antimicrobial susceptibility profile of Escherichia coli O157:H7 from food of animal origin in Bishoftu town, Central Ethiopia

RETRACTED ARTICLE: Occurrence and antimicrobial susceptibility profile of Escherichia coli O157:H7 from food of animal origin in Bishoftu town, Central Ethiopia

Antimicrobial resistance profiles and virulence factors of Escherichia coliO157 collected from a poultry processing plant*

Standardized Escherichia coli O157:H7 Exposure Studies in Cattle Provide Evidence that Bovine Factors Do Not Drive Increased Summertime Colonization

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